Restriction map for
pF1KB8356
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 473 CGCG CG^CG
AccIII 50 TCCGGA T^CCGGA
AfaI 390 GTAC GT^AC
AflIII 935 ACATGT A^C[GA][CT]GT
AocI 295 CCTGAGG CC^T[ACGT]AGG
ApaI 30 GGGCCC GGGCC^C
AspHI 910 GAGCAC G[AT]GC[AT]^C
AvaII 164 GGACC G^G[AT]CC
BalI 818 TGGCCA TGG^CCA
BcgI 393 CGAGAGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 719 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1002 AGATCT A^GATCT
BpmI 519 CTGGAG CTGGAG(16/14)
BsaWI 50 TCCGGA [AT]^CCGG[AT]
BseAI 50 TCCGGA T^CCGGA
BsiHKAI 910 GAGCAC G[AT]GC[AT]^C
BsmI 1046 GCATTC GAATGC(1/-1)
BspEI 50 TCCGGA T^CCGGA
BspLU11I 935 ACATGT A^CATGT
BspMI 627 GCAGGT ACCTGC(4/8)
BstUI 473 CGCG CG^CG
BstXI 329 CCAAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 295 CCTGAGG CC^T[ACGT]AGG
DsaI 459 CCACGG C^C[GA][CT]GG
EarI 799 GAAGAG CTCTTC(1/4)
Eco81I 295 CCTGAGG CC^T[ACGT]AGG
EcoT14I 512 CCAAGG C^C[AT][AT]GG
HphI 175 TCACC GGTGA(8/7)
Ksp632I 799 GAAGAG CTCTTC(1/4)
MluNI 818 TGGCCA TGG^CCA
MroI 50 TCCGGA T^CCGGA
MscI 818 TGGCCA TGG^CCA
MspA1I 579 CAGCGG C[AC]G^C[GT]G
MvnI 473 CGCG CG^CG
NspI 935 ACATGT [GA]CATG^[CT]
PflMI 512 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 172 GACTC GAGTC(4/5)
PpuMI 163 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 390 GTAC GT^AC
SexAI 494 ACCTGGT A^CC[AT]GGT
StyI 512 CCAAGG C^C[AT][AT]GG
TfiI 446 GAATC G^A[AT]TC
Tsp45I 557 GTGAC ^GT[GC]AC
Van91I 512 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 565 GTAGAC GT^[AC][GT]AC
1078 GTAGAC
AlwI 984 GATCC GGATC(4/5)
644 GGATC
AvaI 89 CCCGAG C^[CT]CG[GA]G
610 CCCGAG
BanII 30 GGGCCC G[GA]GC[CT]^C
541 GAGCCC
BcnI 7 CCGGG CC^[GC]GG
157 CCGGG
BsgI 951 CTGCAC GTGCAG(16/14)
625 GTGCAG
BsoBI 89 CCCGAG C^[CT]CG[GA]G
610 CCCGAG
BsrI 1074 ACTGG ACTGG(1/-1)
721 CCAGT
BstF5I 399 GGATG GGATG(2/0)
618 GGATG
BstYI 983 AGATCC [GA]^GATC[CT]
1002 AGATCT
CfoI 44 GCGC GCG^C
651 GCGC
DraII 29 AGGGCCC [GA]G^G[ACGT]CC[CT]
163 GGGACCC
EaeI 818 TGGCCA [CT]^GGCC[GA]
858 TGGCCG
EcoO109I 29 AGGGCCC [GA]G^G[ACGT]CC[CT]
163 GGGACCC
FokI 399 GGATG GGATG(9/13)
618 GGATG
HaeII 43 AGCGCC [GA]GCGC^[CT]
650 AGCGCC
HhaI 44 GCGC GCG^C
651 GCGC
HinP1I 44 GCGC G^CGC
651 GCGC
HinfI 172 GACTC G^A[ACGT]TC
446 GAATC
MaeII 368 ACGT A^CGT
1019 ACGT
MaeIII 557 GTGAC ^GT[ACGT]AC
991 GTTAC
MboII 54 GAAGA GAAGA(8/7)
799 GAAGA
MflI 983 AGATCC [GA]^GATC[CT]
1002 AGATCT
MslI 265 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1047 CATTCTCATG
NciI 7 CCGGG CC^[GC]GG
157 CCGGG
SfcI 143 CTACAG C^T[GA][CT]AG
918 CTACAG
SmlI 179 CTCAAG C^T[CT][GA]AG
1138 CTCAAG
XcmI 966 CCACAGACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
987 CCAGGTTACTTTTGG
XhoII 983 AGATCC [GA]^GATC[CT]
1002 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvCI BbvI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HapII HpaII ItaI MboI
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI TspRI