Restriction map for
pF1KB8354
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AfaI AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BclI BcnI BfaI BglI
BlnI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstPI
BstUI BstXI CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MluI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 829 GTATAC GT^[AC][GT]AC
AccIII 446 TCCGGA T^CCGGA
AflII 739 CTTAAG C^TTAAG
AflIII 1232 ACATGT A^C[GA][CT]GT
AlwNI 44 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1357 CCTGAGG CC^T[ACGT]AGG
Asp700I 937 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 968 TGGCCA TGG^CCA
BanI 16 GGTGCC G^G[CT][GA]CC
BanII 397 GAGCTC G[GA]GC[CT]^C
BcgI 334 GCATATGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 777 GGATAC GTATCC(6/5)
BfrI 739 CTTAAG C^TTAAG
BglII 1169 AGATCT A^GATCT
BpmI 616 CTGGAG CTGGAG(16/14)
BsaAI 1366 CACGTA [CT]AC^GT[GA]
BsaWI 446 TCCGGA [AT]^CCGG[AT]
BseAI 446 TCCGGA T^CCGGA
BspEI 446 TCCGGA T^CCGGA
BspLU11I 1232 ACATGT A^CATGT
BsrBI 1207 GAGCGG CCGCTC(-3/-3)
Bst1107I 829 GTATAC GTA^TAC
BstBI 748 TTCGAA TT^CGAA
BstYI 1169 AGATCT [GA]^GATC[CT]
BstZ17I 829 GTATAC GTA^TAC
Bsu36I 1357 CCTGAGG CC^T[ACGT]AGG
DpnII 1170 GATC ^GATC
DraII 1025 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 968 TGGCCA [CT]^GGCC[GA]
Eco81I 1357 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1025 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 332 ATGCAT ATGCA^T
HapII 447 CCGG C^CGG
HpaII 447 CCGG C^CGG
MaeII 1367 ACGT A^CGT
MboI 1170 GATC ^GATC
MfeI 596 CAATTG C^AATTG
MflI 1169 AGATCT [GA]^GATC[CT]
MluNI 968 TGGCCA TGG^CCA
MroI 446 TCCGGA T^CCGGA
MscI 968 TGGCCA TGG^CCA
MslI 273 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 447 CCGG C^CGG
MunI 596 CAATTG C^AATTG
NdeI 335 CATATG CA^TATG
NdeII 1170 GATC ^GATC
NsiI 332 ATGCAT ATGCA^T
NspV 748 TTCGAA TT^CGAA
PflMI 661 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1102 GAGTC GAGTC(4/5)
PvuII 1081 CAGCTG CAG^CTG
SacI 397 GAGCTC GAGCT^C
Sau3AI 1170 GATC ^GATC
SfaNI 1258 GATGC GCATC(5/9)
SfuI 748 TTCGAA TT^CGAA
SmlI 739 CTTAAG C^T[CT][GA]AG
Van91I 661 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1169 AGATCT [GA]^GATC[CT]
XmnI 937 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 362 AAATTC [GA]^AATT[CT]
694 AAATTT
ApoI 362 AAATTC [GA]^AATT[CT]
694 AAATTT
AspHI 397 GAGCTC G[AT]GC[AT]^C
1198 GAGCAC
AvaII 658 GGACC G^G[AT]CC
1212 GGACC
BbsI 580 GAAGAC GAAGAC(2/6)
1293 GAAGAC
BlpI 55 GCTGAGC GC^T[ACGT]AGC
715 GCTGAGC
BmyI 397 GAGCTC G[AGT]GC[ACT]^C
1198 GAGCAC
Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC
715 GCTGAGC
BpuAI 580 GAAGAC GAAGAC(2/6)
1293 GAAGAC
BsiHKAI 397 GAGCTC G[AT]GC[AT]^C
1198 GAGCAC
BsmAI 149 GAGAC GTCTC(1/5)
353 GTCTC
BsmFI 269 GGGAC GGGAC(10/14)
1211 GGGAC
Bsp1286I 397 GAGCTC G[AGT]GC[ACT]^C
1198 GAGCAC
BspHI 210 TCATGA T^CATGA
1088 TCATGA
BsrDI 1282 GCAATG GCAATG(2/0)
1339 GCAATG
BsrI 1035 ACTGG ACTGG(1/-1)
206 CCAGT
BstF5I 536 GGATG GGATG(2/0)
1257 GGATG
CelII 55 GCTGAGC GC^T[ACGT]AGC
715 GCTGAGC
EarI 1267 GAAGAG CTCTTC(1/4)
1348 GAAGAG
FauI 630 GCGGG CCCGC(4/6)
1209 GCGGG
FokI 536 GGATG GGATG(9/13)
1257 GGATG
HindIII 929 AAGCTT A^AGCTT
985 AAGCTT
Ksp632I 1267 GAAGAG CTCTTC(1/4)
1348 GAAGAG
MseI 100 TTAA T^TAA
740 TTAA
NspI 851 ACATGC [GA]CATG^[CT]
1232 ACATGT
PstI 546 CTGCAG CTGCA^G
1165 CTGCAG
RcaI 210 TCATGA T^CATGA
1088 TCATGA
SfcI 546 CTGCAG C^T[GA][CT]AG
1165 CTGCAG
Tru9I 100 TTAA T^TAA
740 TTAA
XcmI 782 CCAAAGAAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1274 CCAATAAAGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI MaeIII
MboII MnlI MspA1I MvaI MwoI
NlaIII NlaIV Sau96I ScrFI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I