Restriction map for
pF1KB8352
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AspI AviII
BalI BbrPI BciVI BclI BfrI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AcsI 2068 GAATTT [GA]^AATT[CT]
AflIII 1583 ACGTGT A^C[GA][CT]GT
Alw44I 328 GTGCAC G^TGCAC
Aor51HI 257 AGCGCT AGC^GCT
ApaLI 328 GTGCAC G^TGCAC
ApoI 2068 GAATTT [GA]^AATT[CT]
Asp700I 918 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1716 CCTAGG C^CTAGG
BamHI 1849 GGATCC G^GATCC
BbsI 1350 GAAGAC GAAGAC(2/6)
BglII 1493 AGATCT A^GATCT
BlnI 1716 CCTAGG C^CTAGG
BpuAI 1350 GAAGAC GAAGAC(2/6)
BsaAI 1582 TACGTG [CT]AC^GT[GA]
BsaBI 1920 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1324 GAGACC GGTCTC(1/5)
BsmBI 973 CGTCTC CGTCTC(1/5)
BsrFI 1795 GCCGGC [GA]^CCGG[CT]
BssHII 169 GCGCGC G^CGCGC
BssSI 325 CTCGTG CACGAG(-5/-1)
Cfr10I 1795 GCCGGC [GA]^CCGG[CT]
Eco47III 257 AGCGCT AGC^GCT
MamI 1920 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1795 GCCGGC GCC^GGC
NgoMIV 1795 GCCGGC G^CCGGC
PleI 1755 GACTC GAGTC(4/5)
SbfI 645 CCTGCAGG CCTGCA^GG
SexAI 1385 ACCTGGT A^CC[AT]GGT
SmlI 724 CTCAAG C^T[CT][GA]AG
Sse8387I 645 CCTGCAGG CCTGCA^GG
TaqI 1828 TCGA T^CGA
TfiI 1825 GAATC G^A[AT]TC
TthHB8I 1828 TCGA T^CGA
XmnI 918 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AocI 1785 CCTCAGG CC^T[ACGT]AGG
1804 CCTCAGG
BbeI 40 GGCGCC GGCGC^C
183 GGCGCC
BcgI 1819 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1894 GCATCATCGTCG
BfaI 1321 CTAG C^TAG
1717 CTAG
BglI 310 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1715 GCCTAGGCGGC
BsgI 443 CTGCAC GTGCAG(16/14)
1534 CTGCAC
BstXI 129 CCAGTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1859 CCACAGCCGTGG
BstYI 1493 AGATCT [GA]^GATC[CT]
1849 GGATCC
Bsu36I 1785 CCTCAGG CC^T[ACGT]AGG
1804 CCTCAGG
EarI 792 GAAGAG CTCTTC(1/4)
1393 GAAGAG
Eco81I 1785 CCTCAGG CC^T[ACGT]AGG
1804 CCTCAGG
HinfI 1755 GACTC G^A[ACGT]TC
1825 GAATC
HphI 765 GGTGA GGTGA(8/7)
879 TCACC
KasI 40 GGCGCC G^GCGCC
183 GGCGCC
Ksp632I 792 GAAGAG CTCTTC(1/4)
1393 GAAGAG
MaeI 1321 CTAG C^TAG
1717 CTAG
MaeII 1112 ACGT A^CGT
1583 ACGT
MaeIII 878 GTCAC ^GT[ACGT]AC
1706 GTGAC
MflI 1493 AGATCT [GA]^GATC[CT]
1849 GGATCC
MslI 1482 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1860 CACAGCCGTG
NarI 40 GGCGCC GG^CGCC
183 GGCGCC
NcoI 1089 CCATGG C^CATGG
1621 CCATGG
PflMI 1692 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1953 CCAGAAGCTGG
SfoI 40 GGCGCC GGC^GCC
183 GGCGCC
Tsp45I 878 GTCAC ^GT[GC]AC
1706 GTGAC
Tsp509I 1053 AATT ^AATT
2069 AATT
Van91I 1692 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1953 CCAGAAGCTGG
XhoII 1493 AGATCT [GA]^GATC[CT]
1849 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TseI TspRI XcmI
XmaCI XmaI