Restriction map for
pF1KB8347
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SacI SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 889 GTCTAC GT^[AC][GT]AC
AccII 163 CGCG CG^CG
AcyI 955 GACGCC G[GA]^CG[CT]C
Alw44I 608 GTGCAC G^TGCAC
AocI 904 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1001 AGCGCT AGC^GCT
ApaLI 608 GTGCAC G^TGCAC
AspHI 608 GTGCAC G[AT]GC[AT]^C
BalI 665 TGGCCA TGG^CCA
BfaI 589 CTAG C^TAG
BlpI 354 GCTCAGC GC^T[ACGT]AGC
BmyI 608 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 354 GCTCAGC GC^T[ACGT]AGC
BsaHI 955 GACGCC G[GA]^CG[CT]C
BsgI 940 GTGCAG GTGCAG(16/14)
BsiEI 732 CGATCG CG[GA][CT]^CG
BsiHKAI 608 GTGCAC G[AT]GC[AT]^C
BsmBI 52 GAGACG CGTCTC(1/5)
Bsp1286I 608 GTGCAC G[AGT]GC[ACT]^C
BsrBI 283 GAGCGG CCGCTC(-3/-3)
BstAPI 942 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 163 CGCG CG^CG
BstXI 560 CCAATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 904 CCTGAGG CC^T[ACGT]AGG
CelII 354 GCTCAGC GC^T[ACGT]AGC
DraIII 538 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1001 AGCGCT AGC^GCT
Eco81I 904 CCTGAGG CC^T[ACGT]AGG
EcoNI 900 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1052 CCAAGG C^C[AT][AT]GG
FauI 789 GCGGG CCCGC(4/6)
Hin1I 955 GACGCC G[GA]^CG[CT]C
HphI 426 GGTGA GGTGA(8/7)
MaeI 589 CTAG C^TAG
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
MseI 722 TTAA T^TAA
MslI 27 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 163 CGCG CG^CG
PflMI 738 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 636 GACAATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 949 CTGCAG CTGCA^G
PvuI 732 CGATCG CGAT^CG
PvuII 268 CAGCTG CAG^CTG
SapI 327 GAAGAGC GCTCTTC(1/4)
StyI 1052 CCAAGG C^C[AT][AT]GG
Tru9I 722 TTAA T^TAA
TspRI 1128 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 738 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 959 CCATCAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 918 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1093 GAAGCTGTTC
AspI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
877 GACCCCGTC
BbsI 46 GAAGAC GAAGAC(2/6)
402 GAAGAC
BglI 863 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1042 GCCAGCGAGGC
BpuAI 46 GAAGAC GAAGAC(2/6)
402 GAAGAC
BspMI 621 GCAGGT ACCTGC(4/8)
1069 GCAGGT
BstF5I 504 GGATG GGATG(2/0)
1122 GGATG
DpnII 474 GATC ^GATC
733 GATC
EarI 82 CTCTTC CTCTTC(1/4)
327 GAAGAG
FokI 504 GGATG GGATG(9/13)
1122 GGATG
HaeII 245 AGCGCC [GA]GCGC^[CT]
1001 AGCGCT
HinfI 415 GACTC G^A[ACGT]TC
895 GAGTC
Ksp632I 82 CTCTTC CTCTTC(1/4)
327 GAAGAG
MboI 474 GATC ^GATC
733 GATC
NdeII 474 GATC ^GATC
733 GATC
PflFI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
877 GACCCCGTC
PleI 415 GACTC GAGTC(4/5)
895 GAGTC
Sau3AI 474 GATC ^GATC
733 GATC
SexAI 539 ACCTGGT A^CC[AT]GGT
566 ACCTGGT
SfcI 9 CTACAG C^T[GA][CT]AG
949 CTGCAG
SfiI 862 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1041 GGCCAGCGAGGCC
SmaI 104 CCCGGG CCC^GGG
689 CCCGGG
SphI 332 GCATGC GCATG^C
488 GCATGC
Tsp509I 562 AATT ^AATT
650 AATT
Tth111I 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
877 GACCCCGTC
XmaCI 104 CCCGGG C^CCGGG
689 CCCGGG
XmaI 104 CCCGGG C^CCGGG
689 CCCGGG
XmnI 918 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1093 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII AviII BbvI BcnI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII Fnu4HI FspI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MaeII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI PpuMI RsaI
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I