Restriction map for
pF1KB8345
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI AlwI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvI BcgI BciVI BcnI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MamI MluI MroI MspA1I
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 807 AACGTT AA^CGTT
AflII 1734 CTTAAG C^TTAAG
Alw44I 1249 GTGCAC G^TGCAC
AlwNI 112 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1249 GTGCAC G^TGCAC
AspHI 1249 GTGCAC G[AT]GC[AT]^C
AvaII 78 GGACC G^G[AT]CC
BalI 1639 TGGCCA TGG^CCA
BbvCI 369 CCTCAGC CCTCAGC(-5/-2)
BfrI 1734 CTTAAG C^TTAAG
BglII 1848 AGATCT A^GATCT
BmyI 1249 GTGCAC G[AGT]GC[ACT]^C
BsaAI 438 CACGTA [CT]AC^GT[GA]
BsaWI 751 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1249 GTGCAC G[AT]GC[AT]^C
BsiWI 440 CGTACG C^GTACG
BsiYI 641 CCCTCAATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 641 CCCTCAATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1249 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 395 TGTACA T^GTACA
BsrGI 395 TGTACA T^GTACA
BstAPI 1193 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 522 CCAGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1848 AGATCT [GA]^GATC[CT]
DraII 77 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 4 GACTCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1639 TGGCCA [CT]^GGCC[GA]
EcoO109I 77 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1807 GAATTC G^AATTC
EcoT14I 197 CCAAGG C^C[AT][AT]GG
FauI 1202 GCGGG CCCGC(4/6)
Fnu4HI 464 GCCGC GC^[ACGT]GC
HapII 752 CCGG C^CGG
HpaII 752 CCGG C^CGG
ItaI 464 GCCGC GC^[ACGT]GC
MflI 1848 AGATCT [GA]^GATC[CT]
MluNI 1639 TGGCCA TGG^CCA
MscI 1639 TGGCCA TGG^CCA
MspI 752 CCGG C^CGG
MwoI 1193 GCAACCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 77 GGGACC GG[ACGT]^[ACGT]CC
PpuMI 77 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 807 AACGTT AA^CGTT
SpeI 730 ACTAGT A^CTAGT
SspBI 395 TGTACA T^GTACA
StyI 197 CCAAGG C^C[AT][AT]GG
XhoII 1848 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BclI 351 TGATCA T^GATCA
1853 TGATCA
BfaI 731 CTAG C^TAG
1340 CTAG
BsaI 103 GAGACC GGTCTC(1/5)
591 GAGACC
BseRI 1224 CTCCTC GAGGAG(10/8)
46 GAGGAG
BsgI 1669 CTGCAC GTGCAG(16/14)
1815 GTGCAG
BsrDI 693 GCAATG GCAATG(2/0)
1281 GCAATG
EcoT22I 457 ATGCAT ATGCA^T
565 ATGCAT
FbaI 351 TGATCA T^GATCA
1853 TGATCA
MaeI 731 CTAG C^TAG
1340 CTAG
MfeI 1299 CAATTG C^AATTG
1719 CAATTG
MunI 1299 CAATTG C^AATTG
1719 CAATTG
NsiI 457 ATGCAT ATGCA^T
565 ATGCAT
StuI 536 AGGCCT AGG^CCT
956 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I BbsI BpmI BpuAI
BsaJI BsmAI BsmFI BsmI BspLU11I
BsrI BssKI BstBI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FokI HaeIII HindIII HinfI HphI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI NdeI NdeII
NlaIII NspI NspV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XmnI