Restriction map for
pF1KB8344
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BpuAI BsaBI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 808 GAATTT [GA]^AATT[CT]
AflIII 868 ACGTGT A^C[GA][CT]GT
AlwNI 131 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 463 GGGCCC GGGCC^C
ApoI 808 GAATTT [GA]^AATT[CT]
AspHI 654 GAGCTC G[AT]GC[AT]^C
BbrPI 867 CACGTG CAC^GTG
BcnI 243 CCGGG CC^[GC]GG
BglII 488 AGATCT A^GATCT
BlpI 136 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 136 GCTGAGC GC^T[ACGT]AGC
BsaAI 867 CACGTG [CT]AC^GT[GA]
BsaWI 419 ACCGGA [AT]^CCGG[AT]
BsiHKAI 654 GAGCTC G[AT]GC[AT]^C
BsmAI 217 GAGAC GTCTC(1/5)
BssSI 79 CACGAG CACGAG(-5/-1)
BstAPI 602 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 804 CCAGGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 488 AGATCT [GA]^GATC[CT]
CelII 136 GCTGAGC GC^T[ACGT]AGC
DrdI 279 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 145 GCGGG CCCGC(4/6)
HincII 163 GTTAAC GT[CT]^[GA]AC
HindII 163 GTTAAC GT[CT]^[GA]AC
HindIII 794 AAGCTT A^AGCTT
HinfI 357 GACTC G^A[ACGT]TC
HpaI 163 GTTAAC GTT^AAC
MaeII 868 ACGT A^CGT
MflI 488 AGATCT [GA]^GATC[CT]
MseI 164 TTAA T^TAA
NciI 243 CCGGG CC^[GC]GG
PleI 357 GACTC GAGTC(4/5)
PmaCI 867 CACGTG CAC^GTG
PmlI 867 CACGTG CAC^GTG
PpuMI 286 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 654 GAGCTC GAGCT^C
ScaI 41 AGTACT AGT^ACT
Tru9I 164 TTAA T^TAA
XhoII 488 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 197 GGCGCC G[GA]^CG[CT]C
667 GGCGCC
Asp700I 400 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
793 GAAGCTTTTC
BanI 197 GGCGCC G^G[CT][GA]CC
667 GGCGCC
BanII 463 GGGCCC G[GA]GC[CT]^C
654 GAGCTC
BbeI 197 GGCGCC GGCGC^C
667 GGCGCC
BclI 185 TGATCA T^GATCA
260 TGATCA
BsaHI 197 GGCGCC G[GA]^CG[CT]C
667 GGCGCC
BsaJI 93 CCGAGG C^C[ACGT][ACGT]GG
577 CCAGGG
BsmFI 580 GGGAC GGGAC(10/14)
288 GTCCC
BsmI 690 GAATGC GAATGC(1/-1)
572 GCATTC
BspMI 284 GCAGGT ACCTGC(4/8)
477 GCAGGT
BsrI 194 ACTGG ACTGG(1/-1)
494 CCAGT
CfoI 198 GCGC GCG^C
668 GCGC
FbaI 185 TGATCA T^GATCA
260 TGATCA
HaeII 197 GGCGCC [GA]GCGC^[CT]
667 GGCGCC
HaeIII 172 GGCC GG^CC
464 GGCC
HapII 243 CCGG C^CGG
420 CCGG
HhaI 198 GCGC GCG^C
668 GCGC
Hin1I 197 GGCGCC G[GA]^CG[CT]C
667 GGCGCC
HinP1I 198 GCGC G^CGC
668 GCGC
HpaII 243 CCGG C^CGG
420 CCGG
HphI 724 GGTGA GGTGA(8/7)
683 TCACC
KasI 197 GGCGCC G^GCGCC
667 GGCGCC
MslI 292 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
556 CATATTTATG
MspA1I 629 CAGCTG C[AC]G^C[GT]G
760 CAGCTG
MspI 243 CCGG C^CGG
420 CCGG
NarI 197 GGCGCC GG^CGCC
667 GGCGCC
PvuII 629 CAGCTG CAG^CTG
760 CAGCTG
SfaNI 441 GATGC GCATC(5/9)
155 GCATC
SfoI 197 GGCGCC GGC^GCC
667 GGCGCC
Tsp45I 74 GTGAC ^GT[GC]AC
872 GTCAC
XmnI 400 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
793 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BpmI BseRI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI ItaI
Ksp632I MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TseI Tsp509I TspRI