Restriction map for
pF1KB8342
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI BamHI
BbeI BbrPI BbvCI BclI BfrI
BglII BpmI BsaHI BsaI BseRI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MflI MluI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 938 TCCGGA T^CCGGA
ApaI 316 GGGCCC GGGCC^C
AspHI 552 GTGCTC G[AT]GC[AT]^C
AvaI 538 CCCGAG C^[CT]CG[GA]G
AviII 203 TGCGCA TGC^GCA
AvrII 261 CCTAGG C^CTAGG
BalI 392 TGGCCA TGG^CCA
BanI 795 GGTGCC G^G[CT][GA]CC
BcgI 62 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 301 GGATAC GTATCC(6/5)
BcnI 1038 CCCGG CC^[GC]GG
BglI 805 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 261 CCTAGG C^CTAGG
BlpI 94 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 94 GCTGAGC GC^T[ACGT]AGC
BsaBI 648 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 938 TCCGGA [AT]^CCGG[AT]
BseAI 938 TCCGGA T^CCGGA
BsiHKAI 552 GTGCTC G[AT]GC[AT]^C
BsmAI 1021 GAGAC GTCTC(1/5)
BsmBI 1021 GAGACG CGTCTC(1/5)
BsmFI 851 GTCCC GGGAC(10/14)
BsmI 814 GCATTC GAATGC(1/-1)
BsoBI 538 CCCGAG C^[CT]CG[GA]G
BspEI 938 TCCGGA T^CCGGA
BsrBI 400 GAGCGG CCGCTC(-3/-3)
BsrFI 517 GCCGGC [GA]^CCGG[CT]
BsrI 981 ACTGG ACTGG(1/-1)
BssSI 555 CTCGTG CACGAG(-5/-1)
BstEII 747 GGTCACC G^GT[ACGT]ACC
BstPI 747 GGTCACC G^GT[ACGT]ACC
CelII 94 GCTGAGC GC^T[ACGT]AGC
Cfr10I 517 GCCGGC [GA]^CCGG[CT]
DdeI 95 CTGAG C^T[ACGT]AG
DraIII 980 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 250 CTCTTC CTCTTC(1/4)
EcoO65I 747 GGTCACC G^GT[ACGT]ACC
FauI 752 CCCGC CCCGC(4/6)
FspI 203 TGCGCA TGC^GCA
HincII 592 GTCAAC GT[CT]^[GA]AC
HindII 592 GTCAAC GT[CT]^[GA]AC
Ksp632I 250 CTCTTC CTCTTC(1/4)
MamI 648 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 392 TGGCCA TGG^CCA
MroI 938 TCCGGA T^CCGGA
MscI 392 TGGCCA TGG^CCA
MspA1I 698 CCGCTG C[AC]G^C[GT]G
NaeI 517 GCCGGC GCC^GGC
NciI 1038 CCCGG CC^[GC]GG
NcoI 100 CCATGG C^CATGG
NgoMIV 517 GCCGGC G^CCGGC
PstI 547 CTGCAG CTGCA^G
ScaI 83 AGTACT AGT^ACT
SfaNI 326 GATGC GCATC(5/9)
SfcI 547 CTGCAG C^T[GA][CT]AG
SmlI 580 CTCAAG C^T[CT][GA]AG
SphI 947 GCATGC GCATG^C
StuI 995 AGGCCT AGG^CCT
TfiI 563 GAATC G^A[AT]TC
XcmI 511 CCACTCGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 128 ACATGT A^C[GA][CT]GT
617 ACATGT
AluI 200 AGCT AG^CT
674 AGCT
BanII 316 GGGCCC G[GA]GC[CT]^C
837 GGGCTC
BbsI 310 GTCTTC GAAGAC(2/6)
679 GTCTTC
BfaI 235 CTAG C^TAG
262 CTAG
BpuAI 310 GTCTTC GAAGAC(2/6)
679 GTCTTC
BsaAI 28 TACGTG [CT]AC^GT[GA]
406 TACGTG
BspHI 860 TCATGA T^CATGA
920 TCATGA
BspLU11I 128 ACATGT A^CATGT
617 ACATGT
DpnII 141 GATC ^GATC
786 GATC
DraII 15 AGGCCCT [GA]G^G[ACGT]CC[CT]
542 AGGGCCT
DsaI 100 CCATGG C^C[GA][CT]GG
239 CCGTGG
EaeI 119 TGGCCG [CT]^GGCC[GA]
392 TGGCCA
EcoO109I 15 AGGCCCT [GA]G^G[ACGT]CC[CT]
542 AGGGCCT
HaeII 489 GGCGCT [GA]GCGC^[CT]
889 AGCGCC
MaeI 235 CTAG C^TAG
262 CTAG
MboI 141 GATC ^GATC
786 GATC
NdeII 141 GATC ^GATC
786 GATC
PleI 601 GAGTC GAGTC(4/5)
715 GAGTC
RcaI 860 TCATGA T^CATGA
920 TCATGA
Sau3AI 141 GATC ^GATC
786 GATC
Tsp509I 331 AATT ^AATT
366 AATT
TspRI 550 CAGTG CA[GC]TG[ACGT][ACGT]^
980 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MslI MspI MvaI MwoI
NlaIII NlaIV NspI RsaI Sau96I
ScrFI StyI TaqI TseI Tsp45I
TthHB8I