Restriction map for
pF1KB8339
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaII AviII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BpmI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HpaI KspI MamI MluI
MroI MvnI MwoI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 388 GGTACC G^GTACC
AcyI 235 GGCGCC G[GA]^CG[CT]C
Aor51HI 524 AGCGCT AGC^GCT
Asp718I 388 GGTACC G^GTACC
AvaI 584 CTCGGG C^[CT]CG[GA]G
AvrII 538 CCTAGG C^CTAGG
BbeI 235 GGCGCC GGCGC^C
BcnI 575 CCCGG CC^[GC]GG
BfaI 539 CTAG C^TAG
BglII 849 AGATCT A^GATCT
BlnI 538 CCTAGG C^CTAGG
BlpI 604 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 604 GCTCAGC GC^T[ACGT]AGC
BsaHI 235 GGCGCC G[GA]^CG[CT]C
BsmBI 337 GAGACG CGTCTC(1/5)
BsoBI 584 CTCGGG C^[CT]CG[GA]G
Bsp1407I 179 TGTACA T^GTACA
BsrGI 179 TGTACA T^GTACA
BstYI 849 AGATCT [GA]^GATC[CT]
CelII 604 GCTCAGC GC^T[ACGT]AGC
Cfr13I 168 GGGCC G^G[ACGT]CC
DrdI 130 GACCCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 894 CCACGG C^C[GA][CT]GG
EarI 31 GAAGAG CTCTTC(1/4)
Eco47III 524 AGCGCT AGC^GCT
EcoT14I 538 CCTAGG C^C[AT][AT]GG
HapII 576 CCGG C^CGG
Hin1I 235 GGCGCC G[GA]^CG[CT]C
HincII 313 GTTGAC GT[CT]^[GA]AC
HindII 313 GTTGAC GT[CT]^[GA]AC
HindIII 737 AAGCTT A^AGCTT
HpaII 576 CCGG C^CGG
KasI 235 GGCGCC G^GCGCC
KpnI 388 GGTACC GGTAC^C
Ksp632I 31 GAAGAG CTCTTC(1/4)
MaeI 539 CTAG C^TAG
MfeI 530 CAATTG C^AATTG
MflI 849 AGATCT [GA]^GATC[CT]
MseI 854 TTAA T^TAA
MslI 363 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 576 CCGG C^CGG
MunI 530 CAATTG C^AATTG
NarI 235 GGCGCC GG^CGCC
NciI 575 CCCGG CC^[GC]GG
NlaIII 267 CATG CATG^
SacI 878 GAGCTC GAGCT^C
Sau96I 168 GGGCC G^G[ACGT]CC
ScaI 504 AGTACT AGT^ACT
SfoI 235 GGCGCC GGC^GCC
SspBI 179 TGTACA T^GTACA
StyI 538 CCTAGG C^C[AT][AT]GG
Tru9I 854 TTAA T^TAA
Tsp45I 492 GTCAC ^GT[GC]AC
XhoII 849 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 329 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
407 CAGCTGCTG
AspHI 696 GAGCAC G[AT]GC[AT]^C
878 GAGCTC
BalI 216 TGGCCA TGG^CCA
708 TGGCCA
BbsI 76 GAAGAC GAAGAC(2/6)
148 GTCTTC
BpuAI 76 GAAGAC GAAGAC(2/6)
148 GTCTTC
BsiHKAI 696 GAGCAC G[AT]GC[AT]^C
878 GAGCTC
BsmAI 337 GAGAC GTCTC(1/5)
635 GAGAC
CfoI 236 GCGC GCG^C
525 GCGC
EaeI 216 TGGCCA [CT]^GGCC[GA]
708 TGGCCA
HaeII 235 GGCGCC [GA]GCGC^[CT]
524 AGCGCT
HgaI 339 GACGC GACGC(5/10)
624 GCGTC
HhaI 236 GCGC GCG^C
525 GCGC
HinP1I 236 GCGC G^CGC
525 GCGC
MluNI 216 TGGCCA TGG^CCA
708 TGGCCA
MscI 216 TGGCCA TGG^CCA
708 TGGCCA
PleI 543 GACTC GAGTC(4/5)
582 GACTC
SfaNI 307 GATGC GCATC(5/9)
104 GCATC
TaqI 834 TCGA T^CGA
876 TCGA
Tsp509I 476 AATT ^AATT
531 AATT
TthHB8I 834 TCGA T^CGA
876 TCGA
XcmI 359 CCAGCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
711 CCAGAGTAAAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BanII BbvI BmyI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MvaI
NdeII NlaIV PstI PvuII RsaI
Sau3AI ScrFI SfcI TfiI TseI
TspRI