Restriction map for
pF1KB8338
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MroI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 683 ACATGT A^C[GA][CT]GT
AlwI 574 GATCC GGATC(4/5)
AlwNI 649 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 14 GGGCCC GGGCC^C
BalI 2 TGGCCA TGG^CCA
BglI 111 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 752 AGATCT A^GATCT
BlpI 72 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 72 GCTGAGC GC^T[ACGT]AGC
BseRI 482 CTCCTC GAGGAG(10/8)
BsgI 159 GTGCAG GTGCAG(16/14)
BsmAI 557 GAGAC GTCTC(1/5)
BspDI 571 ATCGAT AT^CGAT
BspLU11I 683 ACATGT A^CATGT
BsrBI 549 GAGCGG CCGCTC(-3/-3)
BsrFI 380 GCCGGC [GA]^CCGG[CT]
BssHII 607 GCGCGC G^CGCGC
BssSI 195 CTCGTG CACGAG(-5/-1)
BstYI 752 AGATCT [GA]^GATC[CT]
CelII 72 GCTGAGC GC^T[ACGT]AGC
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
ClaI 571 ATCGAT AT^CGAT
EcoT14I 173 CCAAGG C^C[AT][AT]GG
HinfI 37 GACTC G^A[ACGT]TC
KspI 62 CCGCGG CCGC^GG
MaeIII 425 GTGAC ^GT[ACGT]AC
MboII 783 GAAGA GAAGA(8/7)
MflI 752 AGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 395 TTAA T^TAA
NaeI 380 GCCGGC GCC^GGC
NgoMIV 380 GCCGGC G^CCGGC
PflMI 648 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 37 GACTC GAGTC(4/5)
PpuMI 97 GGGACCC [GA]G^G[AT]CC[CT]
SacII 62 CCGCGG CCGC^GG
StyI 173 CCAAGG C^C[AT][AT]GG
Tru9I 395 TTAA T^TAA
Tsp45I 425 GTGAC ^GT[GC]AC
Van91I 648 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 752 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 71 AGCT AG^CT
163 AGCT
Alw44I 208 GTGCAC G^TGCAC
274 GTGCAC
ApaLI 208 GTGCAC G^TGCAC
274 GTGCAC
AvaII 98 GGACC G^G[AT]CC
699 GGACC
BanII 14 GGGCCC G[GA]GC[CT]^C
337 GAGCCC
BsaAI 31 TACGTG [CT]AC^GT[GA]
271 TACGTG
BstF5I 66 GGATG GGATG(2/0)
773 GGATG
DdeI 73 CTGAG C^T[ACGT]AG
539 CTGAG
DpnII 574 GATC ^GATC
753 GATC
DsaI 62 CCGCGG C^C[GA][CT]GG
290 CCGTGG
FokI 66 GGATG GGATG(9/13)
773 GGATG
HgaI 58 GACGC GACGC(5/10)
129 GACGC
MaeII 32 ACGT A^CGT
272 ACGT
MboI 574 GATC ^GATC
753 GATC
NdeII 574 GATC ^GATC
753 GATC
NspI 320 ACATGC [GA]CATG^[CT]
683 ACATGT
Sau3AI 574 GATC ^GATC
753 GATC
TaqI 572 TCGA T^CGA
720 TCGA
TspRI 9 CACTG CA[GC]TG[ACGT][ACGT]^
206 CAGTG
TthHB8I 572 TCGA T^CGA
720 TCGA
XcmI 8 CCACTGGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
586 CCAGCAGCCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AfaI AspHI
BanI BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfoI TseI