Restriction map for
pF1KB8333
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspI AviII AvrII
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HindIII HpaI Ksp632I
KspI MamI MboII MfeI MluI
MroI MseI MslI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 414 GGTACC G^GTACC
AccII 133 CGCG CG^CG
AfaI 415 GTAC GT^AC
AhdI 820 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 648 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 414 GGTACC G^GTACC
AspEI 820 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 834 GAGCTC G[AT]GC[AT]^C
AvaI 160 CTCGGG C^[CT]CG[GA]G
BamHI 636 GGATCC G^GATCC
BbrPI 850 CACGTG CAC^GTG
BcgI 228 GCAAGTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 577 CCGGG CC^[GC]GG
BpmI 45 CTCCAG CTGGAG(16/14)
BsaAI 850 CACGTG [CT]AC^GT[GA]
BsgI 586 GTGCAG GTGCAG(16/14)
BsiEI 804 CGGCCG CG[GA][CT]^CG
BsiHKAI 834 GAGCTC G[AT]GC[AT]^C
BsmAI 567 GTCTC GTCTC(1/5)
BsmFI 481 GTCCC GGGAC(10/14)
BsmI 319 GCATTC GAATGC(1/-1)
BsoBI 160 CTCGGG C^[CT]CG[GA]G
BspMI 881 ACCTGC ACCTGC(4/8)
BsrDI 66 CATTGC GCAATG(2/0)
BssSI 535 CACGAG CACGAG(-5/-1)
BstUI 133 CGCG CG^CG
DraII 626 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 804 CGGCCG C^GGCCG
Eam1105I 820 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 804 CGGCCG C^GGCCG
Eco52I 804 CGGCCG C^GGCCG
EcoO109I 626 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 352 GATATC GAT^ATC
EcoT14I 549 CCATGG C^C[AT][AT]GG
HincII 268 GTCAAC GT[CT]^[GA]AC
HindII 268 GTCAAC GT[CT]^[GA]AC
HphI 33 GGTGA GGTGA(8/7)
KpnI 414 GGTACC GGTAC^C
MvnI 133 CGCG CG^CG
NciI 577 CCGGG CC^[GC]GG
NcoI 549 CCATGG C^CATGG
NdeI 610 CATATG CA^TATG
PleI 368 GACTC GAGTC(4/5)
PmaCI 850 CACGTG CAC^GTG
PmlI 850 CACGTG CAC^GTG
PpuMI 626 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 415 GTAC GT^AC
SacI 834 GAGCTC GAGCT^C
SbfI 630 CCTGCAGG CCTGCA^GG
Sse8387I 630 CCTGCAGG CCTGCA^GG
StyI 549 CCATGG C^C[AT][AT]GG
TaqI 777 TCGA T^CGA
TfiI 190 GATTC G^A[AT]TC
Tsp509I 689 AATT ^AATT
TthHB8I 777 TCGA T^CGA
XbaI 255 TCTAGA T^CTAGA
XmnI 648 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 849 ACACGT A^C[GA][CT]GT
851 ACGTGT
AvaII 288 GGTCC G^G[AT]CC
627 GGTCC
BalI 18 TGGCCA TGG^CCA
423 TGGCCA
BanII 430 GGGCTC G[GA]GC[CT]^C
834 GAGCTC
BbeI 91 GGCGCC GGCGC^C
545 GGCGCC
BfaI 256 CTAG C^TAG
929 CTAG
BseRI 447 CTCCTC GAGGAG(10/8)
622 GAGGAG
BsrI 37 ACTGG ACTGG(1/-1)
109 ACTGG
BstYI 636 GGATCC [GA]^GATC[CT]
723 AGATCC
CfoI 92 GCGC GCG^C
546 GCGC
DsaI 60 CCGTGG C^C[GA][CT]GG
549 CCATGG
HaeII 91 GGCGCC [GA]GCGC^[CT]
545 GGCGCC
HapII 577 CCGG C^CGG
857 CCGG
HgaI 779 GACGC GACGC(5/10)
252 GCGTC
HhaI 92 GCGC GCG^C
546 GCGC
HinP1I 92 GCGC G^CGC
546 GCGC
HinfI 190 GATTC G^A[ACGT]TC
368 GACTC
HpaII 577 CCGG C^CGG
857 CCGG
KasI 91 GGCGCC G^GCGCC
545 GGCGCC
MaeI 256 CTAG C^TAG
929 CTAG
MaeII 362 ACGT A^CGT
851 ACGT
MflI 636 GGATCC [GA]^GATC[CT]
723 AGATCC
MluNI 18 TGGCCA TGG^CCA
423 TGGCCA
MscI 18 TGGCCA TGG^CCA
423 TGGCCA
MspA1I 419 CAGCTG C[AC]G^C[GT]G
558 CAGCTG
MspI 577 CCGG C^CGG
857 CCGG
NarI 91 GGCGCC GG^CGCC
545 GGCGCC
PstI 463 CTGCAG CTGCA^G
631 CTGCAG
PvuII 419 CAGCTG CAG^CTG
558 CAGCTG
SfcI 463 CTGCAG C^T[GA][CT]AG
631 CTGCAG
SfoI 91 GGCGCC GGC^GCC
545 GGCGCC
TspRI 108 CACTG CA[GC]TG[ACGT][ACGT]^
392 CAGTG
XhoII 636 GGATCC [GA]^GATC[CT]
723 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcyI AluI AlwI BanI
BbvI BmyI BsaHI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII Hin1I
ItaI MaeIII MboI MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I