Restriction map for
pF1KB8331
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvrII
BalI BbrPI BbsI BbvCI BciVI
BclI BfrI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I MaeII MaeIII
MamI MboII MfeI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SfcI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 182 CGCG CG^CG
AcyI 175 GGCGCC G[GA]^CG[CT]C
AfaI 542 GTAC GT^AC
Asp700I 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 210 TGCGCA TGC^GCA
BanII 569 GAGCCC G[GA]GC[CT]^C
BbeI 175 GGCGCC GGCGC^C
BbvI 148 GCAGC GCAGC(8/12)
BfaI 494 CTAG C^TAG
BglII 498 AGATCT A^GATCT
BsaHI 175 GGCGCC G[GA]^CG[CT]C
BsaI 534 GAGACC GGTCTC(1/5)
BseRI 170 GAGGAG GAGGAG(10/8)
BsgI 585 GTGCAG GTGCAG(16/14)
BsmAI 534 GAGAC GTCTC(1/5)
BsmI 65 GCATTC GAATGC(1/-1)
BsrBI 343 CCGCTC CCGCTC(-3/-3)
BsrDI 86 GCAATG GCAATG(2/0)
BstUI 182 CGCG CG^CG
BstXI 573 CCAGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT14I 296 CCATGG C^C[AT][AT]GG
FspI 210 TGCGCA TGC^GCA
Hin1I 175 GGCGCC G[GA]^CG[CT]C
HphI 377 TCACC GGTGA(8/7)
KasI 175 GGCGCC G^GCGCC
KspI 181 CCGCGG CCGC^GG
MaeI 494 CTAG C^TAG
MseI 602 TTAA T^TAA
MspA1I 181 CCGCGG C[AC]G^C[GT]G
MvnI 182 CGCG CG^CG
NarI 175 GGCGCC GG^CGCC
NcoI 296 CCATGG C^CATGG
PpuMI 129 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 542 GTAC GT^AC
SacII 181 CCGCGG CCGC^GG
SexAI 537 ACCTGGT A^CC[AT]GGT
SfiI 49 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 175 GGCGCC GGC^GCC
SmlI 262 CTTGAG C^T[CT][GA]AG
StuI 521 AGGCCT AGG^CCT
StyI 296 CCATGG C^C[AT][AT]GG
TaqI 31 TCGA T^CGA
Tru9I 602 TTAA T^TAA
TseI 148 GCAGC G^C[AT]GC
TthHB8I 31 TCGA T^CGA
XmnI 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AluI 308 AGCT AG^CT
492 AGCT
BamHI 320 GGATCC G^GATCC
466 GGATCC
BcgI 151 GCAAGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
587 GCAGAGGTGTCG
BglI 50 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
77 GCCCAGGGGGC
DdeI 502 CTCAG C^T[ACGT]AG
525 CTCAG
HaeII 175 GGCGCC [GA]GCGC^[CT]
430 GGCGCT
MslI 193 CATGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
273 CATCACGATG
NlaIII 193 CATG CATG^
297 CATG
SmaI 134 CCCGGG CCC^GGG
329 CCCGGG
XmaCI 134 CCCGGG C^CCGGG
329 CCCGGG
XmaI 134 CCCGGG C^CCGGG
329 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AlwI AvaI AvaII BanI
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI XhoII