Restriction map for
pF1KB8329
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeIII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 50 AAATTT [GA]^AATT[CT]
AlwI 597 GGATC GGATC(4/5)
AlwNI 559 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 50 AAATTT [GA]^AATT[CT]
AspHI 150 GAGCAC G[AT]GC[AT]^C
AvaI 326 CCCGAG C^[CT]CG[GA]G
AvaII 69 GGTCC G^G[AT]CC
BalI 410 TGGCCA TGG^CCA
BanI 274 GGTGCC G^G[CT][GA]CC
BbvI 283 GCTGC GCAGC(8/12)
BcnI 588 CCGGG CC^[GC]GG
BglI 24 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 215 AGATCT A^GATCT
BlpI 251 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 251 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 150 GAGCAC G[AT]GC[AT]^C
BsmAI 481 GAGAC GTCTC(1/5)
BsoBI 326 CCCGAG C^[CT]CG[GA]G
BsrBI 161 GAGCGG CCGCTC(-3/-3)
BstAPI 47 GCAAAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 251 GCTTAGC GC^T[ACGT]AGC
DrdI 359 GACAGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 410 TGGCCA [CT]^GGCC[GA]
EcoRV 242 GATATC GAT^ATC
HapII 588 CCGG C^CGG
HpaII 588 CCGG C^CGG
MluNI 410 TGGCCA TGG^CCA
MscI 410 TGGCCA TGG^CCA
MslI 198 CACAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 227 CAGCTG C[AC]G^C[GT]G
MspI 588 CCGG C^CGG
NciI 588 CCGGG CC^[GC]GG
NdeI 449 CATATG CA^TATG
NlaIV 274 GGTGCC GG[ACGT]^[ACGT]CC
PflMI 558 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 86 CTGCAG CTGCA^G
PvuII 227 CAGCTG CAG^CTG
SfaNI 374 GCATC GCATC(5/9)
TseI 283 GCTGC G^C[AT]GC
Van91I 558 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 189 TCTAGA T^CTAGA
XcmI 413 CCAACAAACGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 163 GCGG CCGC(-3/-1)
574 GCGG
AfaI 141 GTAC GT^AC
264 GTAC
BmyI 150 GAGCAC G[AGT]GC[ACT]^C
275 GTGCCC
BsmFI 221 GGGAC GGGAC(10/14)
612 GTCCC
Bsp1286I 150 GAGCAC G[AGT]GC[ACT]^C
275 GTGCCC
BstYI 215 AGATCT [GA]^GATC[CT]
597 GGATCT
Cac8I 43 GCCAGC GC[ACGT]^[ACGT]GC
577 GCAGGC
DdeI 252 CTTAG C^T[ACGT]AG
533 CTAAG
EarI 517 CTCTTC CTCTTC(1/4)
436 GAAGAG
HinfI 82 GAATC G^A[ACGT]TC
561 GAATC
HphI 342 GGTGA GGTGA(8/7)
173 TCACC
Ksp632I 517 CTCTTC CTCTTC(1/4)
436 GAAGAG
MboII 436 GAAGA GAAGA(8/7)
518 TCTTC
MflI 215 AGATCT [GA]^GATC[CT]
597 GGATCT
RsaI 141 GTAC GT^AC
264 GTAC
TfiI 82 GAATC G^A[AT]TC
561 GAATC
Tsp509I 51 AATT ^AATT
65 AATT
XhoII 215 AGATCT [GA]^GATC[CT]
597 GGATCT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BfaI BsaJI BsiYI BslI
BsrI BssKI BstNI Cfr13I DpnII
EcoRII Fnu4HI HaeIII ItaI MaeI
MaeII MboI MnlI MvaI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
SfcI TspRI