Restriction map for
pF1KB8326
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 1042 GGCGTC G[GA]^CG[CT]C
AfaI 948 GTAC GT^AC
Alw44I 574 GTGCAC G^TGCAC
ApaI 665 GGGCCC GGGCC^C
ApaLI 574 GTGCAC G^TGCAC
AvaI 565 CCCGAG C^[CT]CG[GA]G
AviII 218 TGCGCA TGC^GCA
BbsI 624 GTCTTC GAAGAC(2/6)
BbvCI 465 CCTCAGC CCTCAGC(-5/-2)
BpuAI 624 GTCTTC GAAGAC(2/6)
BsaHI 1042 GGCGTC G[GA]^CG[CT]C
BsaWI 798 ACCGGA [AT]^CCGG[AT]
BsgI 112 GTGCAG GTGCAG(16/14)
BsiEI 638 CGGCCG CG[GA][CT]^CG
BsoBI 565 CCCGAG C^[CT]CG[GA]G
BstAPI 210 GCACACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 849 GGATG GGATG(2/0)
BstXI 713 CCAGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 32 AGATCC [GA]^GATC[CT]
EagI 638 CGGCCG C^GGCCG
EarI 805 GAAGAG CTCTTC(1/4)
EclXI 638 CGGCCG C^GGCCG
Eco52I 638 CGGCCG C^GGCCG
EcoT22I 295 ATGCAT ATGCA^T
FokI 849 GGATG GGATG(9/13)
FspI 218 TGCGCA TGC^GCA
Hin1I 1042 GGCGTC G[GA]^CG[CT]C
Ksp632I 805 GAAGAG CTCTTC(1/4)
MflI 32 AGATCC [GA]^GATC[CT]
MslI 294 CATGCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 640 GCCGGC GCC^GGC
NdeI 298 CATATG CA^TATG
NgoMIV 640 GCCGGC G^CCGGC
NsiI 295 ATGCAT ATGCA^T
PleI 602 GACTC GAGTC(4/5)
PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 948 GTAC GT^AC
SacI 976 GAGCTC GAGCT^C
SapI 805 GAAGAGC GCTCTTC(1/4)
SbfI 231 CCTGCAGG CCTGCA^GG
SexAI 188 ACCAGGT A^CC[AT]GGT
SfaNI 509 GCATC GCATC(5/9)
SfiI 174 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 231 CCTGCAGG CCTGCA^GG
Tsp509I 860 AATT ^AATT
XhoII 32 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 33 GATCC GGATC(4/5)
455 GGATC
AspHI 574 GTGCAC G[AT]GC[AT]^C
976 GAGCTC
BalI 173 TGGCCA TGG^CCA
447 TGGCCA
BglI 175 GCCAGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
675 GCCCAAGGGGC
BsiHKAI 574 GTGCAC G[AT]GC[AT]^C
976 GAGCTC
BsmFI 1057 GGGAC GGGAC(10/14)
786 GTCCC
BsrFI 582 GCCGGT [GA]^CCGG[CT]
640 GCCGGC
Cfr10I 582 GCCGGT [GA]^CCGG[CT]
640 GCCGGC
DsaI 149 CCATGG C^C[GA][CT]GG
450 CCATGG
EcoNI 246 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
404 CCTACTGCAGG
FauI 529 CCCGC CCCGC(4/6)
81 GCGGG
HaeII 135 GGCGCT [GA]GCGC^[CT]
646 AGCGCC
HgaI 622 GCGTC GACGC(5/10)
1043 GCGTC
HphI 288 GGTGA GGTGA(8/7)
939 GGTGA
MaeIII 940 GTGAC ^GT[ACGT]AC
1009 GTCAC
MluNI 173 TGGCCA TGG^CCA
447 TGGCCA
MscI 173 TGGCCA TGG^CCA
447 TGGCCA
NcoI 149 CCATGG C^CATGG
450 CCATGG
NspI 221 GCATGT [GA]CATG^[CT]
293 ACATGC
PflMI 165 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
883 CCAGGATTTGG
TaqI 501 TCGA T^CGA
749 TCGA
TfiI 561 GATTC G^A[AT]TC
1064 GATTC
Tsp45I 940 GTGAC ^GT[GC]AC
1009 GTCAC
TthHB8I 501 TCGA T^CGA
749 TCGA
Van91I 165 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
883 CCAGGATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfcI StyI TseI TspRI