Restriction map for
pF1KB8325
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I CpoI DdeI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 412 AAATTT [GA]^AATT[CT]
AluI 226 AGCT AG^CT
Alw44I 239 GTGCAC G^TGCAC
ApaLI 239 GTGCAC G^TGCAC
ApoI 412 AAATTT [GA]^AATT[CT]
Asp700I 422 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 239 GTGCAC G[AT]GC[AT]^C
BbvI 112 GCAGC GCAGC(8/12)
BciVI 186 GGATAC GTATCC(6/5)
BfaI 355 CTAG C^TAG
BmyI 239 GTGCAC G[AGT]GC[ACT]^C
BsaBI 30 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 239 GTGCAC G[AT]GC[AT]^C
BsmI 426 GCATTC GAATGC(1/-1)
Bsp1286I 239 GTGCAC G[AGT]GC[ACT]^C
BspDI 370 ATCGAT AT^CGAT
BsrI 294 ACTGG ACTGG(1/-1)
BssKI 517 CCTGG ^CC[ACGT]GG
BstF5I 156 GGATG GGATG(2/0)
BstNI 517 CCTGG CC^[AT]GG
BstYI 29 AGATCC [GA]^GATC[CT]
Cac8I 68 GCCTGC GC[ACGT]^[ACGT]GC
ClaI 370 ATCGAT AT^CGAT
DraIII 242 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 275 GAAGAG CTCTTC(1/4)
EcoRII 517 CCTGG ^CC[AT]GG
EcoT14I 579 CCAAGG C^C[AT][AT]GG
Fnu4HI 112 GCAGC GC^[ACGT]GC
FokI 156 GGATG GGATG(9/13)
HincII 127 GTTGAC GT[CT]^[GA]AC
HindII 127 GTTGAC GT[CT]^[GA]AC
HinfI 551 GAGTC G^A[ACGT]TC
HphI 138 GGTGA GGTGA(8/7)
ItaI 112 GCAGC GC^[ACGT]GC
Ksp632I 275 GAAGAG CTCTTC(1/4)
MaeI 355 CTAG C^TAG
MamI 30 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 29 AGATCC [GA]^GATC[CT]
MvaI 517 CCTGG CC^[AT]GG
NlaIV 589 GGAGCC GG[ACGT]^[ACGT]CC
NspI 350 ACATGC [GA]CATG^[CT]
PleI 551 GAGTC GAGTC(4/5)
ScrFI 517 CCTGG CC^[ACGT]GG
SmlI 75 CTTGAG C^T[CT][GA]AG
StuI 405 AGGCCT AGG^CCT
StyI 579 CCAAGG C^C[AT][AT]GG
TseI 112 GCAGC G^C[AT]GC
TspRI 152 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 29 AGATCC [GA]^GATC[CT]
XmnI 422 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 329 GTAC GT^AC
472 GTAC
AlwI 30 GATCC GGATC(4/5)
100 GATCC
BsaJI 516 CCCTGG C^C[ACGT][ACGT]GG
579 CCAAGG
BseRI 584 GAGGAG GAGGAG(10/8)
587 GAGGAG
Bsp1407I 328 TGTACA T^GTACA
471 TGTACA
BsrGI 328 TGTACA T^GTACA
471 TGTACA
HaeIII 406 GGCC GG^CC
577 GGCC
MaeII 11 ACGT A^CGT
94 ACGT
MaeIII 268 GTCAC ^GT[ACGT]AC
561 GTCAC
MwoI 59 GCAAGTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
169 GCTAAACTTGC
NlaIII 351 CATG CATG^
432 CATG
RsaI 329 GTAC GT^AC
472 GTAC
SspBI 328 TGTACA T^GTACA
471 TGTACA
TaqI 371 TCGA T^CGA
392 TCGA
Tsp45I 268 GTCAC ^GT[GC]AC
561 GTCAC
TthHB8I 371 TCGA T^CGA
392 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
DpnII MboI MboII MnlI MseI
NdeII Sau3AI Tru9I Tsp509I