Restriction map for
pF1KB8321
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BcnI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MaeII MluI
MluNI MroI MscI MspI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflII 613 CTTAAG C^TTAAG
AflIII 317 ACATGT A^C[GA][CT]GT
AlwI 78 GATCC GGATC(4/5)
AlwNI 187 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 91 GTGCTC G[AT]GC[AT]^C
AspI 445 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 623 GTCTTC GAAGAC(2/6)
BciVI 261 GTATCC GTATCC(6/5)
BclI 639 TGATCA T^GATCA
BfaI 58 CTAG C^TAG
BfrI 613 CTTAAG C^TTAAG
BglII 339 AGATCT A^GATCT
BmyI 91 GTGCTC G[AGT]GC[ACT]^C
BpuAI 623 GTCTTC GAAGAC(2/6)
BsaBI 840 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 400 CTGCAC GTGCAG(16/14)
BsiHKAI 91 GTGCTC G[AT]GC[AT]^C
BsiYI 306 CCTTACAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 306 CCTTACAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 91 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 317 ACATGT A^CATGT
BsrDI 200 GCAATG GCAATG(2/0)
BsrI 50 CCAGT ACTGG(1/-1)
DraI 213 TTTAAA TTT^AAA
DsaI 113 CCATGG C^C[GA][CT]GG
EcoNI 306 CCTTACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 538 GAATTC G^AATTC
EcoT14I 113 CCATGG C^C[AT][AT]GG
FbaI 639 TGATCA T^GATCA
HincII 205 GTTGAC GT[CT]^[GA]AC
HindII 205 GTTGAC GT[CT]^[GA]AC
HphI 154 GGTGA GGTGA(8/7)
MaeI 58 CTAG C^TAG
MamI 840 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 149 CAATTG C^AATTG
MslI 789 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 226 CAGCTG C[AC]G^C[GT]G
MunI 149 CAATTG C^AATTG
NcoI 113 CCATGG C^CATGG
PflFI 445 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 5 CTGCAG CTGCA^G
PvuII 226 CAGCTG CAG^CTG
SapI 597 GAAGAGC GCTCTTC(1/4)
ScaI 761 AGTACT AGT^ACT
SmlI 613 CTTAAG C^T[CT][GA]AG
StyI 113 CCATGG C^C[AT][AT]GG
Tsp45I 443 GTGAC ^GT[GC]AC
Tth111I 445 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 57 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 195 GCGG CCGC(-3/-1)
771 GCGG
AfaI 321 GTAC GT^AC
762 GTAC
Asp700I 538 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
555 GAACCTTTTC
BstYI 77 AGATCC [GA]^GATC[CT]
339 AGATCT
Cac8I 3 GCCTGC GC[ACGT]^[ACGT]GC
679 GCAGGC
MaeIII 98 GTTAC ^GT[ACGT]AC
443 GTGAC
MflI 77 AGATCC [GA]^GATC[CT]
339 AGATCT
NspI 317 ACATGT [GA]CATG^[CT]
866 ACATGC
PleI 688 GACTC GAGTC(4/5)
823 GACTC
RsaI 321 GTAC GT^AC
762 GTAC
SfaNI 754 GATGC GCATC(5/9)
845 GCATC
SfcI 5 CTGCAG C^T[GA][CT]AG
361 CTACAG
TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^
692 CACTG
XhoII 77 AGATCC [GA]^GATC[CT]
339 AGATCT
XmnI 538 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
555 GAACCTTTTC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
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AcsI AluI ApoI BbvI BsaJI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MboI
MboII MnlI MseI MvaI NdeII
NlaIII Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp509I