Restriction map for
pF1KB8318
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AciI 12 GCGG CCGC(-3/-1)
BbrPI 681 CACGTG CAC^GTG
BbvCI 487 GCTGAGG CCTCAGC(-5/-2)
BbvI 586 GCTGC GCAGC(8/12)
BcnI 16 CCCGG CC^[GC]GG
BsaAI 681 CACGTG [CT]AC^GT[GA]
BsiYI 456 CCCACTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 456 CCCACTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 110 TCATGA T^CATGA
Cac8I 8 GCCTGC GC[ACGT]^[ACGT]GC
EarI 735 GAAGAG CTCTTC(1/4)
EcoT14I 289 CCAAGG C^C[AT][AT]GG
HapII 17 CCGG C^CGG
HgaI 432 GCGTC GACGC(5/10)
HincII 154 GTTGAC GT[CT]^[GA]AC
HindII 154 GTTGAC GT[CT]^[GA]AC
HpaII 17 CCGG C^CGG
HphI 300 TCACC GGTGA(8/7)
Ksp632I 735 GAAGAG CTCTTC(1/4)
MaeII 682 ACGT A^CGT
MslI 21 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 17 CCGG C^CGG
MwoI 718 GCAAATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 16 CCCGG CC^[GC]GG
PmaCI 681 CACGTG CAC^GTG
PmlI 681 CACGTG CAC^GTG
RcaI 110 TCATGA T^CATGA
SmlI 23 CTCAAG C^T[CT][GA]AG
StuI 6 AGGCCT AGG^CCT
StyI 289 CCAAGG C^C[AT][AT]GG
TseI 586 GCTGC G^C[AT]GC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 199 GGTACC G^GTACC
293 GGTACC
AflIII 680 ACACGT A^C[GA][CT]GT
682 ACGTGT
AlwI 114 GATCC GGATC(4/5)
475 GATCC
Asp718I 199 GGTACC G^GTACC
293 GGTACC
AvaII 30 GGACC G^G[AT]CC
528 GGACC
BanI 199 GGTACC G^G[CT][GA]CC
293 GGTACC
BbsI 509 GAAGAC GAAGAC(2/6)
691 GAAGAC
BpuAI 509 GAAGAC GAAGAC(2/6)
691 GAAGAC
BseRI 284 CTCCTC GAGGAG(10/8)
259 GAGGAG
BsrI 540 ACTGG ACTGG(1/-1)
499 CCAGT
DpnII 114 GATC ^GATC
475 GATC
EcoRI 424 GAATTC G^AATTC
622 GAATTC
Fnu4HI 12 GCGGC GC^[ACGT]GC
586 GCTGC
HaeIII 7 GGCC GG^CC
14 GGCC
ItaI 12 GCGGC GC^[ACGT]GC
586 GCTGC
KpnI 199 GGTACC GGTAC^C
293 GGTACC
MboI 114 GATC ^GATC
475 GATC
NdeII 114 GATC ^GATC
475 GATC
NlaIII 111 CATG CATG^
159 CATG
Sau3AI 114 GATC ^GATC
475 GATC
Tsp45I 145 GTGAC ^GT[GC]AC
602 GTGAC
TspRI 483 CAGTG CA[GC]TG[ACGT][ACGT]^
539 CACTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaJI
BsmFI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII FokI HinfI MaeIII
MboII MnlI MseI MvaI NlaIV
PleI RsaI Sau96I ScrFI Tru9I
Tsp509I