##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaJI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvaI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       TseI        Tsp45I      Tsp509I     Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII          54  CGCG                 CG^CG
AfaI          144  GTAC                 GT^AC
AvaII          21  GGACC                G^G[AT]CC
BamHI         200  GGATCC               G^GATCC
BsaI          105  GGTCTC               GGTCTC(1/5)
BsaWI          87  TCCGGT               [AT]^CCGG[AT]
BsmAI         106  GTCTC                GTCTC(1/5)
BstF5I          8  GGATG                GGATG(2/0)
BstUI          54  CGCG                 CG^CG
BstYI         200  GGATCC               [GA]^GATC[CT]
Cfr13I         21  GGACC                G^G[ACGT]CC
EcoRV         160  GATATC               GAT^ATC
FokI            8  GGATG                GGATG(9/13)
HaeIII        321  GGCC                 GG^CC
HapII          88  CCGG                 C^CGG
HincII        124  GTTGAC               GT[CT]^[GA]AC
HindII        124  GTTGAC               GT[CT]^[GA]AC
HpaII          88  CCGG                 C^CGG
HphI           90  GGTGA                GGTGA(8/7)
MflI          200  GGATCC               [GA]^GATC[CT]
MnlI           19  GAGG                 CCTC(7/6)
MspA1I        327  CAGCGG               C[AC]G^C[GT]G
MspI           88  CCGG                 C^CGG
MvnI           54  CGCG                 CG^CG
NlaIII        180  CATG                 CATG^
PflMI          24  CCACTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           49  GAGTC                GAGTC(4/5)
RsaI          144  GTAC                 GT^AC
Sau96I         21  GGACC                G^G[ACGT]CC
ScaI          143  AGTACT               AGT^ACT
SfaNI          41  GATGC                GCATC(5/9)
SfcI          324  CTACAG               C^T[GA][CT]AG
TaqI           63  TCGA                 T^CGA
TspRI         165  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        63  TCGA                 T^CGA
Van91I         24  CCACTCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          260  CCAGCCTAACTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         200  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AciI           53  CCGC                 CCGC(-3/-1)
              329  GCGG                
AluI          206  AGCT                 AG^CT
              278  AGCT                
BsmFI         273  GGGAC                GGGAC(10/14)
              250  GTCCC               
TfiI           60  GATTC                G^A[AT]TC
              308  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AlwI        BsiYI       BslI        DdeI        DpnII       
HinfI       MboI        MboII       NdeII       NlaIV       
Sau3AI