Restriction map for
pF1KB8310
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BmyI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI HphI KasI KpnI KspI
MamI MluI MroI MslI NaeI
NarI NciI NcoI NdeI NgoMIV
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 616 GTCTAC GT^[AC][GT]AC
AccII 166 CGCG CG^CG
AciI 135 GCGG CCGC(-3/-1)
AflIII 69 ACGTGT A^C[GA][CT]GT
AocI 328 CCTGAGG CC^T[ACGT]AGG
BalI 123 TGGCCA TGG^CCA
BbsI 391 GAAGAC GAAGAC(2/6)
BbvI 297 GCAGC GCAGC(8/12)
BfaI 1196 CTAG C^TAG
BpuAI 391 GAAGAC GAAGAC(2/6)
BsaI 1006 GAGACC GGTCTC(1/5)
BseRI 331 GAGGAG GAGGAG(10/8)
BspMI 884 ACCTGC ACCTGC(4/8)
BsrFI 207 GCCGGT [GA]^CCGG[CT]
BssSI 76 CTCGTG CACGAG(-5/-1)
BstAPI 297 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 744 TTCGAA TT^CGAA
BstUI 166 CGCG CG^CG
BstYI 930 GGATCT [GA]^GATC[CT]
Bsu36I 328 CCTGAGG CC^T[ACGT]AGG
CfoI 165 GCGC GCG^C
Cfr10I 207 GCCGGT [GA]^CCGG[CT]
DraI 493 TTTAAA TTT^AAA
EaeI 123 TGGCCA [CT]^GGCC[GA]
Eco81I 328 CCTGAGG CC^T[ACGT]AGG
EcoRI 1033 GAATTC G^AATTC
EcoT14I 942 CCTTGG C^C[AT][AT]GG
EcoT22I 913 ATGCAT ATGCA^T
FauI 135 GCGGG CCCGC(4/6)
Fnu4HI 297 GCAGC GC^[ACGT]GC
HhaI 165 GCGC GCG^C
HinP1I 165 GCGC G^CGC
HindIII 187 AAGCTT A^AGCTT
ItaI 297 GCAGC GC^[ACGT]GC
MaeI 1196 CTAG C^TAG
MaeII 69 ACGT A^CGT
MfeI 232 CAATTG C^AATTG
MflI 930 GGATCT [GA]^GATC[CT]
MluNI 123 TGGCCA TGG^CCA
MscI 123 TGGCCA TGG^CCA
MunI 232 CAATTG C^AATTG
MvnI 166 CGCG CG^CG
MwoI 297 GCAGCCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 1195 GCTAGC G^CTAGC
NsiI 913 ATGCAT ATGCA^T
NspI 837 ACATGC [GA]CATG^[CT]
NspV 744 TTCGAA TT^CGAA
ScaI 1128 AGTACT AGT^ACT
SfcI 44 CTACAG C^T[GA][CT]AG
SfuI 744 TTCGAA TT^CGAA
SspI 574 AATATT AAT^ATT
StyI 942 CCTTGG C^C[AT][AT]GG
TaqI 745 TCGA T^CGA
TseI 297 GCAGC G^C[AT]GC
TthHB8I 745 TCGA T^CGA
XhoII 930 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 29 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
298 CAGCCCCTG
BanI 312 GGCACC G^G[CT][GA]CC
871 GGCACC
BlpI 130 GCTCAGC GC^T[ACGT]AGC
499 GCTCAGC
Bpu1102I 130 GCTCAGC GC^T[ACGT]AGC
499 GCTCAGC
BsmFI 1070 GGGAC GGGAC(10/14)
555 GTCCC
CelII 130 GCTCAGC GC^T[ACGT]AGC
499 GCTCAGC
DraII 30 AGGACCT [GA]G^G[ACGT]CC[CT]
1070 GGGACCT
EarI 536 GAAGAG CTCTTC(1/4)
747 GAAGAG
EcoO109I 30 AGGACCT [GA]G^G[ACGT]CC[CT]
1070 GGGACCT
HapII 208 CCGG C^CGG
515 CCGG
HpaII 208 CCGG C^CGG
515 CCGG
Ksp632I 536 GAAGAG CTCTTC(1/4)
747 GAAGAG
MspI 208 CCGG C^CGG
515 CCGG
PleI 81 GACTC GAGTC(4/5)
614 GAGTC
PpuMI 30 AGGACCT [GA]G^G[AT]CC[CT]
1070 GGGACCT
PvuII 502 CAGCTG CAG^CTG
797 CAGCTG
Tsp45I 79 GTGAC ^GT[GC]AC
200 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FokI HaeIII HinfI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
Tsp509I TspRI