Restriction map for
pF1KB8306
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 18 GGTACC G^GTACC
AciI 139 CCGC CCGC(-3/-1)
AcsI 316 GAATTC [GA]^AATT[CT]
AcyI 61 GACGCC G[GA]^CG[CT]C
AlwNI 130 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 316 GAATTC [GA]^AATT[CT]
Asp718I 18 GGTACC G^GTACC
AspHI 253 GAGCTC G[AT]GC[AT]^C
BanI 18 GGTACC G^G[CT][GA]CC
BbsI 85 GAAGAC GAAGAC(2/6)
BclI 2 TGATCA T^GATCA
BlpI 301 GCTGAGC GC^T[ACGT]AGC
BpmI 382 CTGGAG CTGGAG(16/14)
Bpu1102I 301 GCTGAGC GC^T[ACGT]AGC
BpuAI 85 GAAGAC GAAGAC(2/6)
BsaHI 61 GACGCC G[GA]^CG[CT]C
BsiHKAI 253 GAGCTC G[AT]GC[AT]^C
BsmAI 363 GTCTC GTCTC(1/5)
BspHI 147 TCATGA T^CATGA
BspMI 21 ACCTGC ACCTGC(4/8)
BsrDI 258 CATTGC GCAATG(2/0)
BssKI 14 CCAGG ^CC[ACGT]GG
BstBI 392 TTCGAA TT^CGAA
BstF5I 294 GGATG GGATG(2/0)
BstNI 14 CCAGG CC^[AT]GG
BstXI 92 CCATTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 301 GCTGAGC GC^T[ACGT]AGC
Cfr13I 357 GGCCC G^G[ACGT]CC
DsaI 164 CCATGG C^C[GA][CT]GG
EcoRI 316 GAATTC G^AATTC
EcoRII 14 CCAGG ^CC[AT]GG
EcoT14I 164 CCATGG C^C[AT][AT]GG
FbaI 2 TGATCA T^GATCA
FokI 294 GGATG GGATG(9/13)
HaeIII 357 GGCC GG^CC
HgaI 61 GACGC GACGC(5/10)
Hin1I 61 GACGCC G[GA]^CG[CT]C
HphI 227 TCACC GGTGA(8/7)
KpnI 18 GGTACC GGTAC^C
MaeII 346 ACGT A^CGT
MslI 165 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 14 CCAGG CC^[AT]GG
NcoI 164 CCATGG C^CATGG
NlaIV 18 GGTACC GG[ACGT]^[ACGT]CC
NspV 392 TTCGAA TT^CGAA
RcaI 147 TCATGA T^CATGA
SacI 253 GAGCTC GAGCT^C
Sau96I 357 GGCCC G^G[ACGT]CC
ScrFI 14 CCAGG CC^[ACGT]GG
SfcI 127 CTACAG C^T[GA][CT]AG
SfuI 392 TTCGAA TT^CGAA
StyI 164 CCATGG C^C[AT][AT]GG
TspRI 184 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 19 GTAC GT^AC
37 GTAC
BanII 253 GAGCTC G[GA]GC[CT]^C
417 GGGCTC
BbvI 123 GCAGC GCAGC(8/12)
298 GCTGC
BmyI 253 GAGCTC G[AGT]GC[ACT]^C
417 GGGCTC
BseRI 283 GAGGAG GAGGAG(10/8)
334 GAGGAG
Bsp1286I 253 GAGCTC G[AGT]GC[ACT]^C
417 GGGCTC
BsrI 381 ACTGG ACTGG(1/-1)
360 CCAGT
DdeI 66 CTCAG C^T[ACGT]AG
302 CTGAG
DpnII 3 GATC ^GATC
271 GATC
EarI 280 GAAGAG CTCTTC(1/4)
408 GAAGAG
Ksp632I 280 GAAGAG CTCTTC(1/4)
408 GAAGAG
MboI 3 GATC ^GATC
271 GATC
MwoI 123 GCAGCTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
132 GCCCCTGCCGC
NdeII 3 GATC ^GATC
271 GATC
NlaIII 148 CATG CATG^
165 CATG
PleI 88 GACTC GAGTC(4/5)
151 GACTC
RsaI 19 GTAC GT^AC
37 GTAC
Sau3AI 3 GATC ^GATC
271 GATC
SfaNI 295 GATGC GCATC(5/9)
41 GCATC
SmlI 208 CTTGAG C^T[CT][GA]AG
235 CTTGAG
TaqI 320 TCGA T^CGA
393 TCGA
TfiI 160 GAATC G^A[AT]TC
339 GAATC
TseI 123 GCAGC G^C[AT]GC
298 GCTGC
Tsp509I 113 AATT ^AATT
317 AATT
TthHB8I 320 TCGA T^CGA
393 TCGA
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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AluI BsaJI Fnu4HI HinfI ItaI
MboII MnlI