Restriction map for
pF1KB8304
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseRI BsiEI BsmBI BsoBI
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 500 GGTACC G^GTACC
AccIII 638 TCCGGA T^CCGGA
AlwI 641 GGATC GGATC(4/5)
AlwNI 391 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 500 GGTACC G^GTACC
AspHI 506 GAGCTC G[AT]GC[AT]^C
BalI 679 TGGCCA TGG^CCA
BanI 500 GGTACC G^G[CT][GA]CC
BsaAI 39 CACGTA [CT]AC^GT[GA]
BsaI 377 GAGACC GGTCTC(1/5)
BsaWI 638 TCCGGA [AT]^CCGG[AT]
BseAI 638 TCCGGA T^CCGGA
BsiHKAI 506 GAGCTC G[AT]GC[AT]^C
BsiWI 41 CGTACG C^GTACG
BsmI 293 GCATTC GAATGC(1/-1)
Bsp1407I 53 TGTACA T^GTACA
BspEI 638 TCCGGA T^CCGGA
BsrGI 53 TGTACA T^GTACA
BstAPI 792 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 489 GGTGACC G^GT[ACGT]ACC
BstPI 489 GGTGACC G^GT[ACGT]ACC
BstXI 542 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 426 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 91 GCGC GCG^C
EcoO65I 489 GGTGACC G^GT[ACGT]ACC
EcoT14I 830 CCAAGG C^C[AT][AT]GG
HaeII 90 GGCGCT [GA]GCGC^[CT]
HhaI 91 GCGC GCG^C
HinP1I 91 GCGC G^CGC
HincII 72 GTTGAC GT[CT]^[GA]AC
HindII 72 GTTGAC GT[CT]^[GA]AC
KpnI 500 GGTACC GGTAC^C
MaeII 40 ACGT A^CGT
MluNI 679 TGGCCA TGG^CCA
MroI 638 TCCGGA T^CCGGA
MscI 679 TGGCCA TGG^CCA
MslI 543 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 426 GCTAGC G^CTAGC
SacI 506 GAGCTC GAGCT^C
SapI 633 GCTCTTC GCTCTTC(1/4)
ScaI 136 AGTACT AGT^ACT
SexAI 230 ACCTGGT A^CC[AT]GGT
SspBI 53 TGTACA T^GTACA
StyI 830 CCAAGG C^C[AT][AT]GG
TaqI 888 TCGA T^CGA
TthHB8I 888 TCGA T^CGA
XcmI 830 CCAAGGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 105 CCGC CCGC(-3/-1)
819 GCGG
AcsI 241 GAATTT [GA]^AATT[CT]
265 AAATTC
AflII 94 CTTAAG C^TTAAG
163 CTTAAG
ApaI 599 GGGCCC GGGCC^C
728 GGGCCC
ApoI 241 GAATTT [GA]^AATT[CT]
265 AAATTC
AvaII 366 GGTCC G^G[AT]CC
659 GGACC
BcnI 597 CCGGG CC^[GC]GG
731 CCCGG
BfrI 94 CTTAAG C^TTAAG
163 CTTAAG
BglII 152 AGATCT A^GATCT
255 AGATCT
BsgI 247 CTGCAC GTGCAG(16/14)
799 CTGCAC
BsmAI 118 GAGAC GTCTC(1/5)
377 GAGAC
BsmFI 658 GGGAC GGGAC(10/14)
460 GTCCC
EaeI 679 TGGCCA [CT]^GGCC[GA]
827 CGGCCA
EarI 299 CTCTTC CTCTTC(1/4)
634 CTCTTC
Ksp632I 299 CTCTTC CTCTTC(1/4)
634 CTCTTC
MspA1I 328 CAGCTG C[AC]G^C[GT]G
545 CAGCTG
NciI 597 CCGGG CC^[GC]GG
731 CCCGG
NlaIII 270 CATG CATG^
481 CATG
PflMI 12 CCAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
480 CCATGAGGTGG
PpuMI 365 GGGTCCT [GA]G^G[AT]CC[CT]
658 GGGACCC
PvuII 328 CAGCTG CAG^CTG
545 CAGCTG
SfaNI 274 GATGC GCATC(5/9)
572 GCATC
SspI 184 AATATT AAT^ATT
533 AATATT
TfiI 393 GAATC G^A[AT]TC
616 GATTC
Tsp509I 242 AATT ^AATT
266 AATT
TspRI 114 CACTG CA[GC]TG[ACGT][ACGT]^
288 CACTG
Van91I 12 CCAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
480 CCATGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI BanII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SmlI Tru9I TseI
Tsp45I XhoII