Restriction map for
pF1KB8302
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstNI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRII EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI MvaI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TspRI TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwI 50 GATCC GGATC(4/5)
AlwNI 187 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 133 CCTGAGG CC^T[ACGT]AGG
BanI 20 GGTGCC G^G[CT][GA]CC
BanII 27 GAGCCC G[GA]GC[CT]^C
BbrPI 547 CACGTG CAC^GTG
BmyI 27 GAGCCC G[AGT]GC[ACT]^C
BsgI 518 CTGCAC GTGCAG(16/14)
BsmAI 281 GAGAC GTCTC(1/5)
Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C
BsrFI 128 ACCGGC [GA]^CCGG[CT]
BstEII 594 GGTGACC G^GT[ACGT]ACC
BstPI 594 GGTGACC G^GT[ACGT]ACC
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
Cfr10I 128 ACCGGC [GA]^CCGG[CT]
DdeI 134 CTGAG C^T[ACGT]AG
DpnII 50 GATC ^GATC
DraIII 117 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 307 CTCTTC CTCTTC(1/4)
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
EcoNI 432 CCTTAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 594 GGTGACC G^GT[ACGT]ACC
EcoRI 607 GAATTC G^AATTC
Ksp632I 307 CTCTTC CTCTTC(1/4)
MboI 50 GATC ^GATC
NdeII 50 GATC ^GATC
PflMI 564 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 547 CACGTG CAC^GTG
PmlI 547 CACGTG CAC^GTG
PpuMI 188 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 151 CTGCAG CTGCA^G
Sau3AI 50 GATC ^GATC
SmlI 275 CTCAAG C^T[CT][GA]AG
Van91I 564 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 60 CCAGCTGGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 47 CGCG CG^CG
173 CGCG
AflIII 509 ACACGT A^C[GA][CT]GT
546 ACACGT
AspI 69 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
664 GACAATGTC
AvaII 189 GGTCC G^G[AT]CC
647 GGTCC
BcnI 198 CCGGG CC^[GC]GG
525 CCGGG
BpmI 285 CTCCAG CTGGAG(16/14)
142 CTGGAG
BsaAI 510 CACGTA [CT]AC^GT[GA]
547 CACGTG
BseRI 238 GAGGAG GAGGAG(10/8)
529 GAGGAG
BsmFI 81 GTCCC GGGAC(10/14)
218 GTCCC
BspHI 476 TCATGA T^CATGA
641 TCATGA
BspMI 17 GCAGGT ACCTGC(4/8)
186 GCAGGT
BsrI 70 ACTGG ACTGG(1/-1)
264 CCAGT
BssKI 198 CCGGG ^CC[ACGT]GG
525 CCGGG
BstF5I 585 GGATG GGATG(2/0)
630 GGATG
BstUI 47 CGCG CG^CG
173 CGCG
Cac8I 29 GCCCGC GC[ACGT]^[ACGT]GC
39 GCAGGC
CfoI 46 GCGC GCG^C
174 GCGC
DraII 188 AGGTCCT [GA]G^G[ACGT]CC[CT]
533 AGGCCCC
EcoO109I 188 AGGTCCT [GA]G^G[ACGT]CC[CT]
533 AGGCCCC
EcoT14I 455 CCAAGG C^C[AT][AT]GG
538 CCAAGG
FauI 14 CCCGC CCCGC(4/6)
30 CCCGC
FokI 585 GGATG GGATG(9/13)
630 GGATG
HhaI 46 GCGC GCG^C
174 GCGC
HinP1I 46 GCGC G^CGC
174 GCGC
MaeII 511 ACGT A^CGT
548 ACGT
MseI 407 TTAA T^TAA
434 TTAA
MvnI 47 CGCG CG^CG
173 CGCG
MwoI 39 GCAGGCAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
150 GCTGCAGGAGC
NciI 198 CCGGG CC^[GC]GG
525 CCGGG
PflFI 69 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
664 GACAATGTC
PleI 283 GACTC GAGTC(4/5)
616 GAGTC
PvuII 61 CAGCTG CAG^CTG
148 CAGCTG
RcaI 476 TCATGA T^CATGA
641 TCATGA
ScrFI 198 CCGGG CC^[ACGT]GG
525 CCGGG
SfaNI 8 GATGC GCATC(5/9)
449 GCATC
SfcI 151 CTGCAG C^T[GA][CT]AG
392 CTGTAG
StyI 455 CCAAGG C^C[AT][AT]GG
538 CCAAGG
TfiI 109 GAATC G^A[AT]TC
378 GATTC
Tru9I 407 TTAA T^TAA
434 TTAA
Tth111I 69 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
664 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsaJI BsiYI BslI Cfr13I
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI NlaIII NlaIV RsaI
Sau96I TseI Tsp45I Tsp509I