Restriction map for
pF1KB8301
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsiWI BsmI
Bsp1407I BspDI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI MaeII
MamI MluI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SapI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 1029 CGCG CG^CG
AflIII 548 ACATGT A^C[GA][CT]GT
AhdI 1355 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1355 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1078 CGATCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 124 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1014 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1014 GCTGAGC GC^T[ACGT]AGC
BsaI 1196 GGTCTC GGTCTC(1/5)
BsgI 898 GTGCAG GTGCAG(16/14)
BsmBI 1033 GAGACG CGTCTC(1/5)
BspHI 671 TCATGA T^CATGA
BspLU11I 548 ACATGT A^CATGT
BsrBI 104 GAGCGG CCGCTC(-3/-3)
BssSI 725 CTCGTG CACGAG(-5/-1)
BstUI 1029 CGCG CG^CG
BstYI 1094 AGATCC [GA]^GATC[CT]
CelII 1014 GCTGAGC GC^T[ACGT]AGC
DsaI 1028 CCGCGG C^C[GA][CT]GG
Eam1105I 1355 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 922 CTCTTC CTCTTC(1/4)
EcoRV 1322 GATATC GAT^ATC
EcoT14I 1289 CCAAGG C^C[AT][AT]GG
HaeII 7 GGCGCT [GA]GCGC^[CT]
HincII 1352 GTCGAC GT[CT]^[GA]AC
HindII 1352 GTCGAC GT[CT]^[GA]AC
Ksp632I 922 CTCTTC CTCTTC(1/4)
KspI 1028 CCGCGG CCGC^GG
MfeI 822 CAATTG C^AATTG
MflI 1094 AGATCC [GA]^GATC[CT]
MseI 137 TTAA T^TAA
MslI 666 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 822 CAATTG C^AATTG
MvnI 1029 CGCG CG^CG
NheI 987 GCTAGC G^CTAGC
PflFI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 818 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 838 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 150 CGATCG CGAT^CG
RcaI 671 TCATGA T^CATGA
SacI 499 GAGCTC GAGCT^C
SacII 1028 CCGCGG CCGC^GG
SalI 1352 GTCGAC G^TCGAC
ScaI 716 AGTACT AGT^ACT
SfaNI 119 GCATC GCATC(5/9)
SpeI 1383 ACTAGT A^CTAGT
StyI 1289 CCAAGG C^C[AT][AT]GG
TfiI 708 GAATC G^A[AT]TC
Tru9I 137 TTAA T^TAA
Tsp509I 823 AATT ^AATT
Tth111I 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 818 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1094 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 503 TCCGGA T^CCGGA
1200 TCCGGA
AlwI 858 GATCC GGATC(4/5)
1095 GATCC
AspHI 67 GTGCTC G[AT]GC[AT]^C
499 GAGCTC
AvaI 464 CTCGGG C^[CT]CG[GA]G
1068 CTCGGG
BbsI 261 GAAGAC GAAGAC(2/6)
661 GAAGAC
BfaI 988 CTAG C^TAG
1384 CTAG
BpuAI 261 GAAGAC GAAGAC(2/6)
661 GAAGAC
BseAI 503 TCCGGA T^CCGGA
1200 TCCGGA
BseRI 303 CTCCTC GAGGAG(10/8)
1183 GAGGAG
BsiEI 150 CGATCG CG[GA][CT]^CG
1215 CGACCG
BsiHKAI 67 GTGCTC G[AT]GC[AT]^C
499 GAGCTC
BsmAI 1033 GAGAC GTCTC(1/5)
1197 GTCTC
BsoBI 464 CTCGGG C^[CT]CG[GA]G
1068 CTCGGG
BspEI 503 TCCGGA T^CCGGA
1200 TCCGGA
BspMI 578 ACCTGC ACCTGC(4/8)
582 GCAGGT
BsrFI 275 GCCGGT [GA]^CCGG[CT]
983 ACCGGC
BstXI 221 CCAACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
344 CCAAGCCAGTGG
CfoI 8 GCGC GCG^C
18 GCGC
Cfr10I 275 GCCGGT [GA]^CCGG[CT]
983 ACCGGC
DraII 838 GGGTCCC [GA]G^G[ACGT]CC[CT]
1421 AGGCCCT
EcoO109I 838 GGGTCCC [GA]G^G[ACGT]CC[CT]
1421 AGGCCCT
HgaI 699 GCGTC GACGC(5/10)
1023 GCGTC
HhaI 8 GCGC GCG^C
18 GCGC
HinP1I 8 GCGC G^CGC
18 GCGC
MaeI 988 CTAG C^TAG
1384 CTAG
MroI 503 TCCGGA T^CCGGA
1200 TCCGGA
NdeI 489 CATATG CA^TATG
1107 CATATG
PvuII 593 CAGCTG CAG^CTG
1365 CAGCTG
StuI 1229 AGGCCT AGG^CCT
1298 AGGCCT
TaqI 314 TCGA T^CGA
1353 TCGA
TthHB8I 314 TCGA T^CGA
1353 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaJI BsaWI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I SbfI ScrFI SfcI
SmlI Sse8387I TseI Tsp45I TspRI
XcmI