Restriction map for
pF1KB8294
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII KasI KspI MaeII MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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Acc65I 1912 GGTACC G^GTACC
AcsI 888 AAATTT [GA]^AATT[CT]
AlwI 1275 GATCC GGATC(4/5)
Aor51HI 1982 AGCGCT AGC^GCT
ApoI 888 AAATTT [GA]^AATT[CT]
Asp718I 1912 GGTACC G^GTACC
AvrII 981 CCTAGG C^CTAGG
BlnI 981 CCTAGG C^CTAGG
BsaBI 141 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 266 ACCTGC ACCTGC(4/8)
BsrBI 1231 GAGCGG CCGCTC(-3/-3)
BstEII 381 GGTGACC G^GT[ACGT]ACC
BstPI 381 GGTGACC G^GT[ACGT]ACC
BstYI 1274 AGATCC [GA]^GATC[CT]
Eco47III 1982 AGCGCT AGC^GCT
EcoO65I 381 GGTGACC G^GT[ACGT]ACC
HaeII 1982 AGCGCT [GA]GCGC^[CT]
HpaI 1168 GTTAAC GTT^AAC
KpnI 1912 GGTACC GGTAC^C
MamI 141 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1274 AGATCC [GA]^GATC[CT]
PstI 1246 CTGCAG CTGCA^G
SapI 909 GAAGAGC GCTCTTC(1/4)
SmaI 1364 CCCGGG CCC^GGG
XhoII 1274 AGATCC [GA]^GATC[CT]
XmaCI 1364 CCCGGG C^CCGGG
XmaI 1364 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 132 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
943 CAGCGACTG
ApaI 1040 GGGCCC GGGCC^C
1938 GGGCCC
BalI 1097 TGGCCA TGG^CCA
1892 TGGCCA
BbsI 1633 GAAGAC GAAGAC(2/6)
198 GTCTTC
BbvCI 528 GCTGAGG CCTCAGC(-5/-2)
1768 GCTGAGG
BpuAI 1633 GAAGAC GAAGAC(2/6)
198 GTCTTC
BsaI 671 GAGACC GGTCTC(1/5)
1673 GAGACC
BsrFI 659 GCCGGC [GA]^CCGG[CT]
1734 GCCGGC
BstXI 321 CCAGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2022 CCACATGGATGG
CfoI 1103 GCGC GCG^C
1983 GCGC
Cfr10I 659 GCCGGC [GA]^CCGG[CT]
1734 GCCGGC
DpnII 633 GATC ^GATC
1275 GATC
EarI 640 GAAGAG CTCTTC(1/4)
909 GAAGAG
HhaI 1103 GCGC GCG^C
1983 GCGC
HinP1I 1103 GCGC G^CGC
1983 GCGC
HincII 43 GTCAAC GT[CT]^[GA]AC
1168 GTTAAC
HindII 43 GTCAAC GT[CT]^[GA]AC
1168 GTTAAC
Ksp632I 640 GAAGAG CTCTTC(1/4)
909 GAAGAG
MboI 633 GATC ^GATC
1275 GATC
MluNI 1097 TGGCCA TGG^CCA
1892 TGGCCA
MscI 1097 TGGCCA TGG^CCA
1892 TGGCCA
MseI 886 TTAA T^TAA
1169 TTAA
NaeI 659 GCCGGC GCC^GGC
1734 GCCGGC
NcoI 368 CCATGG C^CATGG
624 CCATGG
NdeII 633 GATC ^GATC
1275 GATC
NgoMIV 659 GCCGGC G^CCGGC
1734 GCCGGC
PaeR7I 550 CTCGAG C^TCGAG
1700 CTCGAG
PpuMI 1330 AGGACCC [GA]G^G[AT]CC[CT]
2054 AGGACCC
Sau3AI 633 GATC ^GATC
1275 GATC
SfcI 502 CTACAG C^T[GA][CT]AG
1246 CTGCAG
TfiI 7 GAATC G^A[AT]TC
1375 GATTC
Tru9I 886 TTAA T^TAA
1169 TTAA
Tsp509I 889 AATT ^AATT
1484 AATT
XcmI 77 CCAACACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCATGGATGAACTGG
XhoI 550 CTCGAG C^TCGAG
1700 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CelII Cfr13I DdeI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PflMI PleI PvuII RsaI
SacI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I