Restriction map for
pF1KB8293
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AfaI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 512 GTAGAC GT^[AC][GT]AC
AclI 671 AACGTT AA^CGTT
AflII 709 CTTAAG C^TTAAG
AlwNI 749 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 115 GAGCAC G[AT]GC[AT]^C
AvaII 445 GGACC G^G[AT]CC
BamHI 82 GGATCC G^GATCC
BanII 165 GAGCCC G[GA]GC[CT]^C
BfrI 709 CTTAAG C^TTAAG
BpmI 772 CTGGAG CTGGAG(16/14)
BsiHKAI 115 GAGCAC G[AT]GC[AT]^C
BsrDI 237 CATTGC GCAATG(2/0)
BsrI 730 ACTGG ACTGG(1/-1)
BstXI 764 CCAATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 445 GGACC G^G[ACGT]CC
DraIII 335 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 295 GAAGAG CTCTTC(1/4)
FauI 49 GCGGG CCCGC(4/6)
HaeIII 185 GGCC GG^CC
HgaI 18 GACGC GACGC(5/10)
HincII 208 GTTGAC GT[CT]^[GA]AC
HindII 208 GTTGAC GT[CT]^[GA]AC
HindIII 320 AAGCTT A^AGCTT
HphI 112 GGTGA GGTGA(8/7)
Ksp632I 295 GAAGAG CTCTTC(1/4)
MaeII 672 ACGT A^CGT
MaeIII 486 GTCAC ^GT[ACGT]AC
NlaIII 101 CATG CATG^
NlaIV 82 GGATCC GG[ACGT]^[ACGT]CC
NspI 100 ACATGC [GA]CATG^[CT]
Psp1406I 671 AACGTT AA^CGTT
PstI 58 CTGCAG CTGCA^G
Sau96I 445 GGACC G^G[ACGT]CC
SfaNI 526 GATGC GCATC(5/9)
SpeI 589 ACTAGT A^CTAGT
StuI 184 AGGCCT AGG^CCT
Tsp45I 486 GTCAC ^GT[GC]AC
TspRI 22 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 621 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 269 AAATTC [GA]^AATT[CT]
819 AAATTC
AluI 321 AGCT AG^CT
371 AGCT
AlwI 83 GATCC GGATC(4/5)
82 GGATC
ApoI 269 AAATTC [GA]^AATT[CT]
819 AAATTC
Asp700I 496 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
688 GAAGACATTC
BbsI 688 GAAGAC GAAGAC(2/6)
726 GAAGAC
BglII 255 AGATCT A^GATCT
618 AGATCT
BmyI 115 GAGCAC G[AGT]GC[ACT]^C
165 GAGCCC
BpuAI 688 GAAGAC GAAGAC(2/6)
726 GAAGAC
BsaJI 168 CCCAGG C^C[ACGT][ACGT]GG
350 CCAGGG
BsgI 332 CTGCAC GTGCAG(16/14)
400 GTGCAG
BsmI 653 GAATGC GAATGC(1/-1)
879 GCATTC
Bsp1286I 115 GAGCAC G[AGT]GC[ACT]^C
165 GAGCCC
BssKI 169 CCAGG ^CC[ACGT]GG
350 CCAGG
BstBI 460 TTCGAA TT^CGAA
503 TTCGAA
BstNI 169 CCAGG CC^[AT]GG
350 CCAGG
EcoRII 169 CCAGG ^CC[AT]GG
350 CCAGG
MvaI 169 CCAGG CC^[AT]GG
350 CCAGG
NspV 460 TTCGAA TT^CGAA
503 TTCGAA
PleI 515 GACTC GAGTC(4/5)
900 GACTC
ScrFI 169 CCAGG CC^[ACGT]GG
350 CCAGG
SfcI 58 CTGCAG C^T[GA][CT]AG
530 CTACAG
SfuI 460 TTCGAA TT^CGAA
503 TTCGAA
SmlI 105 CTCAAG C^T[CT][GA]AG
709 CTTAAG
XmnI 496 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
688 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BbvI BfaI BstF5I BstYI
DdeI DpnII Fnu4HI FokI HinfI
ItaI MaeI MboI MboII MflI
MnlI MseI MwoI NdeII Sau3AI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XhoII