Restriction map for
pF1KB8288
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AviII AvrII BbeI BbrPI BbvCI
BciVI BfaI BfrI BlnI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmI BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 345 TCCGGA T^CCGGA
AcsI 94 GAATTC [GA]^AATT[CT]
AhdI 1023 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 94 GAATTC [GA]^AATT[CT]
AseI 442 ATTAAT AT^TAAT
AsnI 442 ATTAAT AT^TAAT
AspEI 1023 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 913 GTGCTC G[AT]GC[AT]^C
AspI 843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 966 CTCGGG C^[CT]CG[GA]G
BanI 922 GGCACC G^G[CT][GA]CC
BbsI 511 GAAGAC GAAGAC(2/6)
BclI 545 TGATCA T^GATCA
BglII 662 AGATCT A^GATCT
BlpI 174 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC
BpuAI 511 GAAGAC GAAGAC(2/6)
BsaWI 345 TCCGGA [AT]^CCGG[AT]
BseAI 345 TCCGGA T^CCGGA
BsiHKAI 913 GTGCTC G[AT]GC[AT]^C
BsoBI 966 CTCGGG C^[CT]CG[GA]G
Bsp1407I 242 TGTACA T^GTACA
BspEI 345 TCCGGA T^CCGGA
BsrFI 588 GCCGGT [GA]^CCGG[CT]
BsrGI 242 TGTACA T^GTACA
BssSI 393 CACGAG CACGAG(-5/-1)
BstAPI 36 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 174 GCTGAGC GC^T[ACGT]AGC
CfoI 901 GCGC GCG^C
Cfr10I 588 GCCGGT [GA]^CCGG[CT]
DrdI 571 GACAAGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 524 CCGTGG C^C[GA][CT]GG
Eam1105I 1023 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 94 GAATTC G^AATTC
FauI 906 CCCGC CCCGC(4/6)
FbaI 545 TGATCA T^GATCA
HaeII 900 AGCGCC [GA]GCGC^[CT]
HhaI 901 GCGC GCG^C
HinP1I 901 GCGC G^CGC
MroI 345 TCCGGA T^CCGGA
MslI 1149 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1148 ACATGC [GA]CATG^[CT]
PflFI 843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 125 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 442 ATTAAT AT^TAAT
ScaI 1191 AGTACT AGT^ACT
SspBI 242 TGTACA T^GTACA
StuI 420 AGGCCT AGG^CCT
TaqI 82 TCGA T^CGA
TfiI 805 GATTC G^A[AT]TC
Tth111I 843 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 82 TCGA T^CGA
Van91I 125 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 288 CGCG CG^CG
355 CGCG
AvaII 77 GGACC G^G[AT]CC
725 GGTCC
BalI 122 TGGCCA TGG^CCA
1143 TGGCCA
BamHI 279 GGATCC G^GATCC
970 GGATCC
BcgI 1028 CGAGTCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
923 GCACCGACATCG
BcnI 261 CCCGG CC^[GC]GG
303 CCGGG
BglI 469 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
634 GCCTTCCTGGC
BsmBI 341 CGTCTC CGTCTC(1/5)
651 CGTCTC
BstF5I 411 CATCC GGATG(2/0)
701 CATCC
BstUI 288 CGCG CG^CG
355 CGCG
BstXI 584 CCAGGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
881 CCAAAGGCTTGG
Cac8I 790 GCCTGC GC[ACGT]^[ACGT]GC
1112 GCACGC
DraIII 15 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
286 CACGCGGTG
EarI 775 CTCTTC CTCTTC(1/4)
857 CTCTTC
FokI 411 CATCC GGATG(9/13)
701 CATCC
Ksp632I 775 CTCTTC CTCTTC(1/4)
857 CTCTTC
MaeII 578 ACGT A^CGT
610 ACGT
MaeIII 556 GTGAC ^GT[ACGT]AC
580 GTCAC
MluNI 122 TGGCCA TGG^CCA
1143 TGGCCA
MscI 122 TGGCCA TGG^CCA
1143 TGGCCA
MspA1I 907 CCGCTG C[AC]G^C[GT]G
1081 CCGCTG
MvnI 288 CGCG CG^CG
355 CGCG
NciI 261 CCCGG CC^[GC]GG
303 CCGGG
PstI 319 CTGCAG CTGCA^G
451 CTGCAG
SfcI 319 CTGCAG C^T[GA][CT]AG
451 CTGCAG
Tsp45I 556 GTGAC ^GT[GC]AC
580 GTCAC
Tsp509I 95 AATT ^AATT
445 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI BanII BbvI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI Tru9I TseI TspRI
XhoII