Restriction map for
pF1KB8286
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT14I
EcoT22I FbaI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 482 AACGTT AA^CGTT
AflII 942 CTTAAG C^TTAAG
AflIII 397 ACACGT A^C[GA][CT]GT
AocI 14 CCTTAGG CC^T[ACGT]AGG
Asp700I 321 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 741 GAGCAC G[AT]GC[AT]^C
BanI 63 GGCACC G^G[CT][GA]CC
BbvI 658 GCAGC GCAGC(8/12)
BfrI 942 CTTAAG C^TTAAG
BmyI 741 GAGCAC G[AGT]GC[ACT]^C
BpmI 281 CTGGAG CTGGAG(16/14)
BsaAI 398 CACGTA [CT]AC^GT[GA]
BsaJI 1045 CCTCGG C^C[ACGT][ACGT]GG
BsiHKAI 741 GAGCAC G[AT]GC[AT]^C
BsiYI 497 CCTTTTTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 497 CCTTTTTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 741 GAGCAC G[AGT]GC[ACT]^C
BsrFI 661 GCCGGT [GA]^CCGG[CT]
BssSI 386 CACGAG CACGAG(-5/-1)
BstYI 429 GGATCT [GA]^GATC[CT]
Bsu36I 14 CCTTAGG CC^T[ACGT]AGG
Cfr10I 661 GCCGGT [GA]^CCGG[CT]
Eco81I 14 CCTTAGG CC^T[ACGT]AGG
EcoRI 769 GAATTC G^AATTC
EcoRV 380 GATATC GAT^ATC
Fnu4HI 658 GCAGC GC^[ACGT]GC
HapII 662 CCGG C^CGG
HpaII 662 CCGG C^CGG
HphI 753 GGTGA GGTGA(8/7)
ItaI 658 GCAGC GC^[ACGT]GC
MflI 429 GGATCT [GA]^GATC[CT]
MspI 662 CCGG C^CGG
NdeI 82 CATATG CA^TATG
NlaIV 63 GGCACC GG[ACGT]^[ACGT]CC
PleI 123 GACTC GAGTC(4/5)
Psp1406I 482 AACGTT AA^CGTT
SfaNI 368 GCATC GCATC(5/9)
SspI 472 AATATT AAT^ATT
TseI 658 GCAGC G^C[AT]GC
XcmI 67 CCAGGAAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 429 GGATCT [GA]^GATC[CT]
XmnI 321 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 689 CCGC CCGC(-3/-1)
750 GCGG
AcsI 646 GAATTT [GA]^AATT[CT]
769 GAATTC
AlwI 685 GATCC GGATC(4/5)
429 GGATC
AlwNI 313 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
830 CAGGAGCTG
ApoI 646 GAATTT [GA]^AATT[CT]
769 GAATTC
AvaI 406 CCCGAG C^[CT]CG[GA]G
1046 CTCGGG
BsoBI 406 CCCGAG C^[CT]CG[GA]G
1046 CTCGGG
DraII 218 AGGACCT [GA]G^G[ACGT]CC[CT]
314 AGGACCT
EarI 1136 CTCTTC CTCTTC(1/4)
922 GAAGAG
EcoO109I 218 AGGACCT [GA]G^G[ACGT]CC[CT]
314 AGGACCT
FauI 688 CCCGC CCCGC(4/6)
750 GCGGG
HincII 588 GTTGAC GT[CT]^[GA]AC
780 GTCAAC
HindII 588 GTTGAC GT[CT]^[GA]AC
780 GTCAAC
Ksp632I 1136 CTCTTC CTCTTC(1/4)
922 GAAGAG
MaeII 399 ACGT A^CGT
483 ACGT
NheI 346 GCTAGC G^CTAGC
364 GCTAGC
NlaIII 261 CATG CATG^
1005 CATG
PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT]
314 AGGACCT
ScaI 1069 AGTACT AGT^ACT
1120 AGTACT
SmlI 942 CTTAAG C^T[CT][GA]AG
1116 CTTGAG
Tsp45I 448 GTGAC ^GT[GC]AC
677 GTCAC
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
798 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AvaII BfaI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI EcoRII FokI HinfI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII RsaI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
Tsp509I TthHB8I