Restriction map for
pF1KB8280
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# NO CUT ENZYMES ( 165 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Cac8I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HindIII HpaI HpaII KasI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 437 GACGTC GACGT^C
Acc65I 551 GGTACC G^GTACC
AciI 461 GCGG CCGC(-3/-1)
AcsI 33 AAATTC [GA]^AATT[CT]
AcyI 437 GACGTC G[GA]^CG[CT]C
AlwNI 380 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 33 AAATTC [GA]^AATT[CT]
Asp718I 551 GGTACC G^GTACC
BanI 551 GGTACC G^G[CT][GA]CC
BbvI 246 GCTGC GCAGC(8/12)
BciVI 412 GGATAC GTATCC(6/5)
BglII 83 AGATCT A^GATCT
BpmI 125 CTGGAG CTGGAG(16/14)
BsaHI 437 GACGTC G[GA]^CG[CT]C
BseRI 292 GAGGAG GAGGAG(10/8)
BsgI 427 GTGCAG GTGCAG(16/14)
BsrI 480 CCAGT ACTGG(1/-1)
BssSI 112 CTCGTG CACGAG(-5/-1)
BstF5I 351 GGATG GGATG(2/0)
BstXI 398 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 83 AGATCT [GA]^GATC[CT]
CfoI 359 GCGC GCG^C
DraII 373 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 462 CGGCCA [CT]^GGCC[GA]
EcoO109I 373 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 351 GGATG GGATG(9/13)
HaeII 358 GGCGCT [GA]GCGC^[CT]
HgaI 15 GCGTC GACGC(5/10)
HhaI 359 GCGC GCG^C
Hin1I 437 GACGTC G[GA]^CG[CT]C
HinP1I 359 GCGC G^CGC
HincII 208 GTTGAC GT[CT]^[GA]AC
HindII 208 GTTGAC GT[CT]^[GA]AC
HphI 276 GGTGA GGTGA(8/7)
KpnI 551 GGTACC GGTAC^C
MflI 83 AGATCT [GA]^GATC[CT]
MslI 399 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 267 CAGCTG C[AC]G^C[GT]G
MwoI 359 GCGCTACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 551 GGTACC GG[ACGT]^[ACGT]CC
PvuII 267 CAGCTG CAG^CTG
StuI 366 AGGCCT AGG^CCT
TseI 246 GCTGC G^C[AT]GC
XbaI 37 TCTAGA T^CTAGA
XcmI 104 CCATAGAACTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 83 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AluI 45 AGCT AG^CT
268 AGCT
AocI 369 CCTCAGG CC^T[ACGT]AGG
377 CCTCAGG
AvaI 59 CTCGGG C^[CT]CG[GA]G
79 CCCGAG
AvaII 141 GGACC G^G[AT]CC
255 GGACC
BbsI 221 GAAGAC GAAGAC(2/6)
469 GTCTTC
BfaI 38 CTAG C^TAG
239 CTAG
BpuAI 221 GAAGAC GAAGAC(2/6)
469 GTCTTC
BsmAI 311 GAGAC GTCTC(1/5)
440 GTCTC
BsmBI 439 CGTCTC CGTCTC(1/5)
311 GAGACG
BsmFI 305 GGGAC GGGAC(10/14)
395 GTCCC
BsoBI 59 CTCGGG C^[CT]CG[GA]G
79 CCCGAG
Bsu36I 369 CCTCAGG CC^T[ACGT]AGG
377 CCTCAGG
Eco81I 369 CCTCAGG CC^T[ACGT]AGG
377 CCTCAGG
EcoT14I 89 CCTTGG C^C[AT][AT]GG
226 CCAAGG
Fnu4HI 246 GCTGC GC^[ACGT]GC
461 GCGGC
ItaI 246 GCTGC GC^[ACGT]GC
461 GCGGC
MaeI 38 CTAG C^TAG
239 CTAG
MaeII 314 ACGT A^CGT
438 ACGT
MaeIII 284 GTGAC ^GT[ACGT]AC
322 GTGAC
PleI 183 GACTC GAGTC(4/5)
193 GACTC
SfcI 362 CTACAG C^T[GA][CT]AG
474 CTGTAG
StyI 89 CCTTGG C^C[AT][AT]GG
226 CCAAGG
TfiI 154 GAATC G^A[AT]TC
493 GATTC
Tsp45I 284 GTGAC ^GT[GC]AC
322 GTGAC
Tsp509I 34 AATT ^AATT
135 AATT
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
481 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI BsaJI BsmI BssKI BstNI
Cfr13I DdeI DpnII EcoRII HaeIII
HinfI MboI MboII MnlI MvaI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI