Restriction map for
pF1KB8275
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 370 GTAGAC GT^[AC][GT]AC
AccII 153 CGCG CG^CG
AcyI 205 GACGCC G[GA]^CG[CT]C
AfaI 600 GTAC GT^AC
AvaII 137 GGACC G^G[AT]CC
BanI 163 GGCACC G^G[CT][GA]CC
BciVI 877 GTATCC GTATCC(6/5)
BfaI 812 CTAG C^TAG
BglI 85 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 333 AGATCT A^GATCT
BsaHI 205 GACGCC G[GA]^CG[CT]C
BsgI 375 CTGCAC GTGCAG(16/14)
BsrFI 537 GCCGGT [GA]^CCGG[CT]
BssSI 836 CTCGTG CACGAG(-5/-1)
BstEII 850 GGTGACC G^GT[ACGT]ACC
BstF5I 225 GGATG GGATG(2/0)
BstNI 881 CCAGG CC^[AT]GG
BstPI 850 GGTGACC G^GT[ACGT]ACC
BstUI 153 CGCG CG^CG
CfoI 179 GCGC GCG^C
Cfr10I 537 GCCGGT [GA]^CCGG[CT]
DraI 433 TTTAAA TTT^AAA
EcoNI 394 CCTATCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 850 GGTGACC G^GT[ACGT]ACC
EcoRII 881 CCAGG ^CC[AT]GG
FokI 225 GGATG GGATG(9/13)
HaeII 178 AGCGCC [GA]GCGC^[CT]
HhaI 179 GCGC GCG^C
Hin1I 205 GACGCC G[GA]^CG[CT]C
HinP1I 179 GCGC G^CGC
MaeI 812 CTAG C^TAG
MaeIII 851 GTGAC ^GT[ACGT]AC
MvaI 881 CCAGG CC^[AT]GG
MvnI 153 CGCG CG^CG
PleI 184 GAGTC GAGTC(4/5)
RsaI 600 GTAC GT^AC
SapI 830 GAAGAGC GCTCTTC(1/4)
ScaI 599 AGTACT AGT^ACT
StuI 306 AGGCCT AGG^CCT
TaqI 775 TCGA T^CGA
Tsp45I 851 GTGAC ^GT[GC]AC
TthHB8I 775 TCGA T^CGA
XbaI 811 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 355 AAATTT [GA]^AATT[CT]
548 AAATTT
ApoI 355 AAATTT [GA]^AATT[CT]
548 AAATTT
AspHI 634 GAGCTC G[AT]GC[AT]^C
833 GAGCTC
BanII 634 GAGCTC G[GA]GC[CT]^C
833 GAGCTC
BcnI 134 CCGGG CC^[GC]GG
140 CCGGG
BmyI 634 GAGCTC G[AGT]GC[ACT]^C
833 GAGCTC
BpmI 569 CTCCAG CTGGAG(16/14)
158 CTGGAG
BsiHKAI 634 GAGCTC G[AT]GC[AT]^C
833 GAGCTC
Bsp1286I 634 GAGCTC G[AGT]GC[ACT]^C
833 GAGCTC
BsrI 209 CCAGT ACTGG(1/-1)
703 CCAGT
BstXI 732 CCACAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCAGCAGAGTGG
Cac8I 67 GCGGGC GC[ACGT]^[ACGT]GC
71 GCGAGC
Cfr13I 137 GGACC G^G[ACGT]CC
512 GGGCC
DdeI 310 CTTAG C^T[ACGT]AG
745 CTTAG
DsaI 86 CCATGG C^C[GA][CT]GG
641 CCATGG
EcoT14I 86 CCATGG C^C[AT][AT]GG
641 CCATGG
HgaI 205 GACGC GACGC(5/10)
154 GCGTC
NciI 134 CCGGG CC^[GC]GG
140 CCGGG
NcoI 86 CCATGG C^CATGG
641 CCATGG
SacI 634 GAGCTC GAGCT^C
833 GAGCTC
Sau96I 137 GGACC G^G[ACGT]CC
512 GGGCC
StyI 86 CCATGG C^C[AT][AT]GG
641 CCATGG
TfiI 439 GAATC G^A[AT]TC
625 GAATC
TspRI 704 CAGTG CA[GC]TG[ACGT][ACGT]^
802 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmFI BssKI
BstYI DpnII EarI FauI Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MseI MspA1I MspI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
Tru9I TseI Tsp509I XhoII