Restriction map for
pF1KB8270
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# NO CUT ENZYMES ( 155 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 709 GACGTC GACGT^C
AgeI 548 ACCGGT A^CCGGT
AviII 605 TGCGCA TGC^GCA
BbvCI 649 GCTGAGG CCTCAGC(-5/-2)
BcgI 467 GCAGGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 55 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 548 ACCGGT [AT]^CCGG[AT]
BseRI 112 CTCCTC GAGGAG(10/8)
BsgI 737 CTGCAC GTGCAG(16/14)
BsmAI 358 GAGAC GTCTC(1/5)
BsmBI 358 GAGACG CGTCTC(1/5)
BspHI 449 TCATGA T^CATGA
BstEII 666 GGTCACC G^GT[ACGT]ACC
BstPI 666 GGTCACC G^GT[ACGT]ACC
DpnII 423 GATC ^GATC
DraII 443 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 443 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 666 GGTCACC G^GT[ACGT]ACC
FauI 755 GCGGG CCCGC(4/6)
FspI 605 TGCGCA TGC^GCA
MboI 423 GATC ^GATC
MslI 763 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 278 CCATGG C^CATGG
NdeII 423 GATC ^GATC
PinAI 548 ACCGGT A^CCGGT
RcaI 449 TCATGA T^CATGA
SapI 747 GAAGAGC GCTCTTC(1/4)
Sau3AI 423 GATC ^GATC
ScaI 251 AGTACT AGT^ACT
TfiI 29 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 33 CGCG CG^CG
601 CGCG
AcyI 709 GACGTC G[GA]^CG[CT]C
727 GACGCC
AvaI 271 CCCGAG C^[CT]CG[GA]G
703 CCCGAG
BanII 274 GAGCCC G[GA]GC[CT]^C
573 GGGCTC
BsaHI 709 GACGTC G[GA]^CG[CT]C
727 GACGCC
BsoBI 271 CCCGAG C^[CT]CG[GA]G
703 CCCGAG
BspMI 118 ACCTGC ACCTGC(4/8)
467 GCAGGT
BstUI 33 CGCG CG^CG
601 CGCG
DdeI 434 CTGAG C^T[ACGT]AG
650 CTGAG
EarI 801 CTCTTC CTCTTC(1/4)
747 GAAGAG
HaeII 136 AGCGCC [GA]GCGC^[CT]
692 AGCGCC
HgaI 727 GACGC GACGC(5/10)
260 GCGTC
Hin1I 709 GACGTC G[GA]^CG[CT]C
727 GACGCC
HphI 116 TCACC GGTGA(8/7)
668 TCACC
Ksp632I 801 CTCTTC CTCTTC(1/4)
747 GAAGAG
MaeII 710 ACGT A^CGT
785 ACGT
MspA1I 159 CCGCTG C[AC]G^C[GT]G
753 CAGCGG
MvnI 33 CGCG CG^CG
601 CGCG
NaeI 67 GCCGGC GCC^GGC
730 GCCGGC
NgoMIV 67 GCCGGC G^CCGGC
730 GCCGGC
SfaNI 193 GATGC GCATC(5/9)
641 GCATC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI BanI BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI StyI TseI
Tsp45I TspRI