Restriction map for
pF1KB8247
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI HphI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 3 GTAC GT^AC
Aor51HI 215 AGCGCT AGC^GCT
AspHI 94 GAGCTC G[AT]GC[AT]^C
AvaI 268 CCCGAG C^[CT]CG[GA]G
BamHI 587 GGATCC G^GATCC
BbsI 697 GAAGAC GAAGAC(2/6)
BfaI 82 CTAG C^TAG
BpuAI 697 GAAGAC GAAGAC(2/6)
BseRI 307 CTCCTC GAGGAG(10/8)
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
BsoBI 268 CCCGAG C^[CT]CG[GA]G
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BstXI 716 CCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 587 GGATCC [GA]^GATC[CT]
CfoI 216 GCGC GCG^C
DraIII 403 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 215 AGCGCT AGC^GCT
HaeII 215 AGCGCT [GA]GCGC^[CT]
HhaI 216 GCGC GCG^C
HinP1I 216 GCGC G^CGC
HincII 71 GTCGAC GT[CT]^[GA]AC
HindII 71 GTCGAC GT[CT]^[GA]AC
MaeI 82 CTAG C^TAG
MflI 587 GGATCC [GA]^GATC[CT]
MspA1I 754 CAGCTG C[AC]G^C[GT]G
NlaIII 222 CATG CATG^
PflMI 288 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 644 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 384 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 754 CAGCTG CAG^CTG
RsaI 3 GTAC GT^AC
SacI 94 GAGCTC GAGCT^C
SalI 71 GTCGAC G^TCGAC
SapI 61 GAAGAGC GCTCTTC(1/4)
SfcI 666 CTACAG C^T[GA][CT]AG
StuI 551 AGGCCT AGG^CCT
Tsp45I 155 GTGAC ^GT[GC]AC
Tsp509I 144 AATT ^AATT
Van91I 288 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 587 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 71 GTCGAC GT^[AC][GT]AC
391 GTCTAC
AlwI 588 GATCC GGATC(4/5)
587 GGATC
ApaI 173 GGGCCC GGGCC^C
651 GGGCCC
AvaII 119 GGACC G^G[AT]CC
645 GGTCC
BalI 458 TGGCCA TGG^CCA
725 TGGCCA
EaeI 458 TGGCCA [CT]^GGCC[GA]
725 TGGCCA
EcoT14I 371 CCAAGG C^C[AT][AT]GG
461 CCAAGG
FauI 475 CCCGC CCCGC(4/6)
56 GCGGG
HinfI 74 GACTC G^A[ACGT]TC
271 GAGTC
MaeIII 155 GTGAC ^GT[ACGT]AC
467 GTAAC
MluNI 458 TGGCCA TGG^CCA
725 TGGCCA
MscI 458 TGGCCA TGG^CCA
725 TGGCCA
MwoI 185 GCATCCTACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
684 GCAGCAGAAGC
PleI 74 GACTC GAGTC(4/5)
271 GAGTC
SfaNI 185 GCATC GCATC(5/9)
542 GCATC
SmlI 13 CTCAAG C^T[CT][GA]AG
76 CTCAAG
StyI 371 CCAAGG C^C[AT][AT]GG
461 CCAAGG
TspRI 407 CAGTG CA[GC]TG[ACGT][ACGT]^
573 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI Ksp632I MboI MboII MnlI
MspI MvaI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
TthHB8I