Restriction map for
pF1KB8243
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AspHI 592 GAGCAC G[AT]GC[AT]^C
AviII 6 TGCGCA TGC^GCA
BanII 16 GAGCCC G[GA]GC[CT]^C
BbsI 409 GTCTTC GAAGAC(2/6)
BcgI 441 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 627 CCCGG CC^[GC]GG
BglI 303 GCCTAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 318 AGATCT A^GATCT
BpuAI 409 GTCTTC GAAGAC(2/6)
BsaI 509 GAGACC GGTCTC(1/5)
BsiHKAI 592 GAGCAC G[AT]GC[AT]^C
BsmAI 509 GAGAC GTCTC(1/5)
BsmFI 191 GGGAC GGGAC(10/14)
BsrFI 199 GCCGGC [GA]^CCGG[CT]
BstYI 318 AGATCT [GA]^GATC[CT]
CfoI 7 GCGC GCG^C
Cfr10I 199 GCCGGC [GA]^CCGG[CT]
DpnII 319 GATC ^GATC
DraII 168 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 201 CGGCCA [CT]^GGCC[GA]
EcoO109I 168 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 125 CCTTGG C^C[AT][AT]GG
FspI 6 TGCGCA TGC^GCA
HaeIII 202 GGCC GG^CC
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HinfI 426 GAATC G^A[ACGT]TC
MaeII 84 ACGT A^CGT
MboI 319 GATC ^GATC
MflI 318 AGATCT [GA]^GATC[CT]
MslI 258 CATCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 199 GCCGGC GCC^GGC
NciI 627 CCCGG CC^[GC]GG
NdeII 319 GATC ^GATC
NgoMIV 199 GCCGGC G^CCGGC
NlaIV 567 GGACCC GG[ACGT]^[ACGT]CC
PpuMI 168 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 319 GATC ^GATC
ScaI 434 AGTACT AGT^ACT
StyI 125 CCTTGG C^C[AT][AT]GG
TaqI 280 TCGA T^CGA
TfiI 426 GAATC G^A[AT]TC
TspRI 250 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 280 TCGA T^CGA
XhoII 318 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 500 AGCT AG^CT
573 AGCT
BbvI 580 GCAGC GCAGC(8/12)
4 GCTGC
BfaI 148 CTAG C^TAG
494 CTAG
BmyI 16 GAGCCC G[AGT]GC[ACT]^C
592 GAGCAC
BsaJI 20 CCCAGG C^C[ACGT][ACGT]GG
125 CCTTGG
Bsp1286I 16 GAGCCC G[AGT]GC[ACT]^C
592 GAGCAC
BstNI 21 CCAGG CC^[AT]GG
204 CCAGG
EcoRII 21 CCAGG ^CC[AT]GG
204 CCAGG
Fnu4HI 4 GCTGC GC^[ACGT]GC
580 GCAGC
HapII 200 CCGG C^CGG
628 CCGG
HpaII 200 CCGG C^CGG
628 CCGG
HphI 49 GGTGA GGTGA(8/7)
97 GGTGA
ItaI 4 GCTGC GC^[ACGT]GC
580 GCAGC
MaeI 148 CTAG C^TAG
494 CTAG
MseI 176 TTAA T^TAA
377 TTAA
MspI 200 CCGG C^CGG
628 CCGG
MvaI 21 CCAGG CC^[AT]GG
204 CCAGG
NlaIII 534 CATG CATG^
564 CATG
Tru9I 176 TTAA T^TAA
377 TTAA
TseI 4 GCTGC G^C[AT]GC
580 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AfaI ApoI AvaII BsiYI
BslI BsrI BssKI BstF5I Cac8I
Cfr13I DdeI FokI MaeIII MboII
MnlI MwoI RsaI Sau96I ScrFI
Tsp509I