Restriction map for
pF1KB8228
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmI BspDI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MscI MseI MslI
MunI MvnI NarI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tru9I Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 88 AAATTC [GA]^AATT[CT]
AcyI 783 GACGCC G[GA]^CG[CT]C
ApoI 88 AAATTC [GA]^AATT[CT]
AspHI 238 GAGCTC G[AT]GC[AT]^C
BamHI 920 GGATCC G^GATCC
BanII 238 GAGCTC G[GA]GC[CT]^C
BbsI 577 GAAGAC GAAGAC(2/6)
BbvCI 871 GCTGAGG CCTCAGC(-5/-2)
BglI 646 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 238 GAGCTC G[AGT]GC[ACT]^C
BpuAI 577 GAAGAC GAAGAC(2/6)
BsaHI 783 GACGCC G[GA]^CG[CT]C
BsgI 56 CTGCAC GTGCAG(16/14)
BsiHKAI 238 GAGCTC G[AT]GC[AT]^C
BsmBI 1060 GAGACG CGTCTC(1/5)
Bsp1286I 238 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 1145 TGTACA T^GTACA
BspMI 1160 ACCTGC ACCTGC(4/8)
BsrBI 671 GAGCGG CCGCTC(-3/-3)
BsrFI 662 GCCGGC [GA]^CCGG[CT]
BsrGI 1145 TGTACA T^GTACA
BstAPI 1047 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 423 GGTCACC G^GT[ACGT]ACC
BstPI 423 GGTCACC G^GT[ACGT]ACC
CfoI 806 GCGC GCG^C
Cfr10I 662 GCCGGC [GA]^CCGG[CT]
EcoO65I 423 GGTCACC G^GT[ACGT]ACC
HgaI 783 GACGC GACGC(5/10)
HhaI 806 GCGC GCG^C
Hin1I 783 GACGCC G[GA]^CG[CT]C
HinP1I 806 GCGC G^CGC
NaeI 662 GCCGGC GCC^GGC
NgoMIV 662 GCCGGC G^CCGGC
PaeR7I 461 CTCGAG C^TCGAG
PflMI 924 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 238 GAGCTC GAGCT^C
SexAI 929 ACCTGGT A^CC[AT]GGT
SmaI 903 CCCGGG CCC^GGG
SmlI 461 CTCGAG C^T[CT][GA]AG
SspBI 1145 TGTACA T^GTACA
SspI 193 AATATT AAT^ATT
TspRI 341 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 924 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 461 CTCGAG C^TCGAG
XmaCI 903 CCCGGG C^CCGGG
XmaI 903 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 83 TCCGGA T^CCGGA
242 TCCGGA
AfaI 1146 GTAC GT^AC
1209 GTAC
AocI 34 CCTCAGG CC^T[ACGT]AGG
589 CCTGAGG
BanI 318 GGCACC G^G[CT][GA]CC
768 GGCACC
BsaI 1139 GAGACC GGTCTC(1/5)
1022 GGTCTC
BseAI 83 TCCGGA T^CCGGA
242 TCCGGA
BspEI 83 TCCGGA T^CCGGA
242 TCCGGA
BsrI 945 ACTGG ACTGG(1/-1)
984 ACTGG
BstYI 821 AGATCC [GA]^GATC[CT]
920 GGATCC
Bsu36I 34 CCTCAGG CC^T[ACGT]AGG
589 CCTGAGG
Eco81I 34 CCTCAGG CC^T[ACGT]AGG
589 CCTGAGG
HincII 625 GTTGAC GT[CT]^[GA]AC
780 GTTGAC
HindII 625 GTTGAC GT[CT]^[GA]AC
780 GTTGAC
HphI 392 TCACC GGTGA(8/7)
425 TCACC
MflI 821 AGATCC [GA]^GATC[CT]
920 GGATCC
MroI 83 TCCGGA T^CCGGA
242 TCCGGA
PstI 1117 CTGCAG CTGCA^G
1162 CTGCAG
RsaI 1146 GTAC GT^AC
1209 GTAC
SapI 235 GAAGAGC GCTCTTC(1/4)
1215 GAAGAGC
SfcI 1117 CTGCAG C^T[GA][CT]AG
1162 CTGCAG
TaqI 462 TCGA T^CGA
1090 TCGA
TfiI 361 GAATC G^A[AT]TC
1033 GATTC
Tsp45I 218 GTGAC ^GT[GC]AC
424 GTCAC
TthHB8I 462 TCGA T^CGA
1090 TCGA
XhoII 821 AGATCC [GA]^GATC[CT]
920 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BbvI BcnI BpmI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsoBI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PleI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp509I