##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII           7  CGCG                 CG^CG
AcyI          364  GACGCC               G[GA]^CG[CT]C
AfaI          539  GTAC                 GT^AC
AlwI          378  GATCC                GGATC(4/5)
AlwNI         272  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          382  CCCGGG               C^[CT]CG[GA]G
BglI            4  GCCCGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          416  CTGGAG               CTGGAG(16/14)
BsaHI         364  GACGCC               G[GA]^CG[CT]C
BsmI          510  GAATGC               GAATGC(1/-1)
BsoBI         382  CCCGGG               C^[CT]CG[GA]G
BsrDI         435  CATTGC               GCAATG(2/0)
BsrI          140  CCAGT                ACTGG(1/-1)
BstNI         370  CCAGG                CC^[AT]GG
BstUI           7  CGCG                 CG^CG
Cfr13I          3  GGCCC                G^G[ACGT]CC
DdeI          162  CTTAG                C^T[ACGT]AG
DpnII         378  GATC                 ^GATC
EcoRII        370  CCAGG                ^CC[AT]GG
HaeII          21  GGCGCT               [GA]GCGC^[CT]
HgaI          364  GACGC                GACGC(5/10)
Hin1I         364  GACGCC               G[GA]^CG[CT]C
KspI            6  CCGCGG               CCGC^GG
MboI          378  GATC                 ^GATC
MslI          505  CACCGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          370  CCAGG                CC^[AT]GG
MvnI            7  CGCG                 CG^CG
NdeII         378  GATC                 ^GATC
NlaIII        495  CATG                 CATG^
NlaIV         418  GGAGCC               GG[ACGT]^[ACGT]CC
PvuII         527  CAGCTG               CAG^CTG
RsaI          539  GTAC                 GT^AC
SacII           6  CCGCGG               CCGC^GG
SapI           30  GCTCTTC              GCTCTTC(1/4)
Sau3AI        378  GATC                 ^GATC
Sau96I          3  GGCCC                G^G[ACGT]CC
SmaI          382  CCCGGG               CCC^GGG
TaqI           77  TCGA                 T^CGA
TthHB8I        77  TCGA                 T^CGA
XmaCI         382  CCCGGG               C^CCGGG
XmaI          382  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BfaI          251  CTAG                 C^TAG
              428  CTAG                
BsaWI         259  TCCGGT               [AT]^CCGG[AT]
              506  ACCGGA              
CfoI           16  GCGC                 GCG^C
               22  GCGC                
DsaI            6  CCGCGG               C^C[GA][CT]GG
              410  CCGTGG              
EarI           31  CTCTTC               CTCTTC(1/4)
              355  GAAGAG              
FauI            5  CCCGC                CCCGC(4/6)
                8  GCGGG               
HhaI           16  GCGC                 GCG^C
               22  GCGC                
HinP1I         16  GCGC                 G^CGC
               22  GCGC                
Ksp632I        31  CTCTTC               CTCTTC(1/4)
              355  GAAGAG              
MaeI          251  CTAG                 C^TAG
              428  CTAG                
MboII         355  GAAGA                GAAGA(8/7)
               32  TCTTC               
MspA1I          6  CCGCGG               C[AC]G^C[GT]G
              527  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      Cac8I       
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MnlI        MspI        MwoI        NciI        
ScrFI       SfaNI       TseI        TspRI