Restriction map for
pF1KB8221
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BpmI BsaAI
BsaBI BsaI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CpoI DraI DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MscI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 51 CGCG CG^CG
AccIII 213 TCCGGA T^CCGGA
AhdI 607 GACGAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 739 GTGCAC G^TGCAC
ApaI 181 GGGCCC GGGCC^C
ApaLI 739 GTGCAC G^TGCAC
AspEI 607 GACGAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 739 GTGCAC G[AT]GC[AT]^C
AvaI 178 CCCGGG C^[CT]CG[GA]G
BamHI 12 GGATCC G^GATCC
BanII 181 GGGCCC G[GA]GC[CT]^C
BbsI 85 GAAGAC GAAGAC(2/6)
BciVI 326 GTATCC GTATCC(6/5)
BglI 100 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BpuAI 85 GAAGAC GAAGAC(2/6)
BsaWI 213 TCCGGA [AT]^CCGG[AT]
BseAI 213 TCCGGA T^CCGGA
BsiHKAI 739 GTGCAC G[AT]GC[AT]^C
BsmAI 691 GTCTC GTCTC(1/5)
BsoBI 178 CCCGGG C^[CT]CG[GA]G
BspDI 302 ATCGAT AT^CGAT
BspEI 213 TCCGGA T^CCGGA
BstUI 51 CGCG CG^CG
BstYI 12 GGATCC [GA]^GATC[CT]
CelII 4 GCTGAGC GC^T[ACGT]AGC
ClaI 302 ATCGAT AT^CGAT
DraIII 624 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 68 TGGCCG [CT]^GGCC[GA]
Eam1105I 607 GACGAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 497 AAGCTT A^AGCTT
HinfI 610 GAGTC G^A[ACGT]TC
HphI 241 GGTGA GGTGA(8/7)
MaeII 524 ACGT A^CGT
MflI 12 GGATCC [GA]^GATC[CT]
MroI 213 TCCGGA T^CCGGA
MseI 659 TTAA T^TAA
MspA1I 733 CCGCTG C[AC]G^C[GT]G
MvnI 51 CGCG CG^CG
NlaIII 699 CATG CATG^
NspI 698 GCATGC [GA]CATG^[CT]
PleI 610 GAGTC GAGTC(4/5)
SgrAI 75 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 178 CCCGGG CCC^GGG
SmlI 721 CTCAAG C^T[CT][GA]AG
SphI 698 GCATGC GCATG^C
StuI 149 AGGCCT AGG^CCT
Tru9I 659 TTAA T^TAA
Tsp509I 379 AATT ^AATT
TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 12 GGATCC [GA]^GATC[CT]
XmaCI 178 CCCGGG C^CCGGG
XmaI 178 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 314 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
620 CAGGCACTG
BmyI 181 GGGCCC G[AGT]GC[ACT]^C
739 GTGCAC
BsmFI 47 GTCCC GGGAC(10/14)
615 GTCCC
Bsp1286I 181 GGGCCC G[AGT]GC[ACT]^C
739 GTGCAC
DpnII 13 GATC ^GATC
301 GATC
DraII 181 GGGCCCC [GA]G^G[ACGT]CC[CT]
728 AGGCCCC
EcoNI 567 CCTAGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
720 CCTCAAGCAGG
EcoO109I 181 GGGCCCC [GA]G^G[ACGT]CC[CT]
728 AGGCCCC
MaeIII 538 GTCAC ^GT[ACGT]AC
653 GTGAC
MboI 13 GATC ^GATC
301 GATC
NdeII 13 GATC ^GATC
301 GATC
Sau3AI 13 GATC ^GATC
301 GATC
SfcI 460 CTACAG C^T[GA][CT]AG
561 CTACAG
TaqI 137 TCGA T^CGA
303 TCGA
Tsp45I 538 GTCAC ^GT[GC]AC
653 GTGAC
TthHB8I 137 TCGA T^CGA
303 TCGA
XcmI 252 CCAGGAAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
619 CCAGGCACTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaII
BanI BbeI BbvI BcnI BfaI
BsaHI BsaJI BsiYI BslI BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI MaeI MboII MnlI MspI
MvaI MwoI NaeI NarI NciI
NgoMIV NlaIV Sau96I ScrFI SfaNI
SfoI TseI