Restriction map for
pF1KB8220
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# NO CUT ENZYMES ( 113 enzymes) #
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AccIII AclI AcsI AflII AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MaeIII MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 340 GACGTC GACGT^C
Acc65I 767 GGTACC G^GTACC
AccI 343 GTCTAC GT^[AC][GT]AC
Asp718I 767 GGTACC G^GTACC
BbeI 857 GGCGCC GGCGC^C
BbrPI 949 CACGTG CAC^GTG
BbsI 1230 GAAGAC GAAGAC(2/6)
BfaI 1108 CTAG C^TAG
BglI 535 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1230 GAAGAC GAAGAC(2/6)
BsaAI 949 CACGTG [CT]AC^GT[GA]
BsaI 190 GAGACC GGTCTC(1/5)
BsgI 1141 GTGCAG GTGCAG(16/14)
BsmI 122 GAATGC GAATGC(1/-1)
BsrDI 730 GCAATG GCAATG(2/0)
BsrFI 854 ACCGGC [GA]^CCGG[CT]
BssSI 610 CACGAG CACGAG(-5/-1)
BstAPI 169 GCACTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 326 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 854 ACCGGC [GA]^CCGG[CT]
DsaI 1235 CCGTGG C^C[GA][CT]GG
EcoT14I 317 CCAAGG C^C[AT][AT]GG
EcoT22I 748 ATGCAT ATGCA^T
KasI 857 GGCGCC G^GCGCC
KpnI 767 GGTACC GGTAC^C
MaeI 1108 CTAG C^TAG
MseI 1262 TTAA T^TAA
NarI 857 GGCGCC GG^CGCC
NsiI 748 ATGCAT ATGCA^T
NspI 746 ACATGC [GA]CATG^[CT]
PmaCI 949 CACGTG CAC^GTG
PmlI 949 CACGTG CAC^GTG
PstI 1159 CTGCAG CTGCA^G
SacI 223 GAGCTC GAGCT^C
SfoI 857 GGCGCC GGC^GCC
StyI 317 CCAAGG C^C[AT][AT]GG
TfiI 1164 GATTC G^A[AT]TC
Tru9I 1262 TTAA T^TAA
XcmI 476 CCAACCTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 768 GTAC GT^AC
1044 GTAC
AocI 47 CCTCAGG CC^T[ACGT]AGG
480 CCTGAGG
AspHI 223 GAGCTC G[AT]GC[AT]^C
1279 GAGCAC
AvaI 143 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BalI 443 TGGCCA TGG^CCA
677 TGGCCA
BanII 223 GAGCTC G[GA]GC[CT]^C
322 GAGCCC
BlpI 295 GCTGAGC GC^T[ACGT]AGC
352 GCTGAGC
Bpu1102I 295 GCTGAGC GC^T[ACGT]AGC
352 GCTGAGC
BsaWI 590 TCCGGT [AT]^CCGG[AT]
1124 ACCGGA
BsiHKAI 223 GAGCTC G[AT]GC[AT]^C
1279 GAGCAC
BsmBI 42 CGTCTC CGTCTC(1/5)
9 GAGACG
BsmFI 422 GGGAC GGGAC(10/14)
912 GTCCC
BsoBI 143 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
Bsu36I 47 CCTCAGG CC^T[ACGT]AGG
480 CCTGAGG
CelII 295 GCTGAGC GC^T[ACGT]AGC
352 GCTGAGC
EarI 760 GAAGAG CTCTTC(1/4)
997 GAAGAG
Eco81I 47 CCTCAGG CC^T[ACGT]AGG
480 CCTGAGG
Ksp632I 760 GAAGAG CTCTTC(1/4)
997 GAAGAG
MluNI 443 TGGCCA TGG^CCA
677 TGGCCA
MscI 443 TGGCCA TGG^CCA
677 TGGCCA
PvuII 307 CAGCTG CAG^CTG
640 CAGCTG
RsaI 768 GTAC GT^AC
1044 GTAC
SmaI 143 CCCGGG CCC^GGG
194 CCCGGG
TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^
447 CACTG
XmaCI 143 CCCGGG C^CCGGG
194 CCCGGG
XmaI 143 CCCGGG C^CCGGG
194 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AhdI AluI
AlwI AlwNI AspEI AvaII BanI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI Eam1105I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI TthHB8I XhoII