Restriction map for
pF1KB8219
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfaI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 142 CGCG CG^CG
AflII 43 CTTAAG C^TTAAG
AflIII 299 ACATGT A^C[GA][CT]GT
ApaI 391 GGGCCC GGGCC^C
Asp700I 75 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 279 GGACC G^G[AT]CC
AviII 182 TGCGCA TGC^GCA
BalI 308 TGGCCA TGG^CCA
BclI 479 TGATCA T^GATCA
BcnI 246 CCGGG CC^[GC]GG
BfrI 43 CTTAAG C^TTAAG
BsaBI 64 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 913 GAGGAG GAGGAG(10/8)
BsgI 472 GTGCAG GTGCAG(16/14)
BsmBI 760 GAGACG CGTCTC(1/5)
BsmI 633 GCATTC GAATGC(1/-1)
BspLU11I 299 ACATGT A^CATGT
BspMI 570 GCAGGT ACCTGC(4/8)
BstUI 142 CGCG CG^CG
BstXI 375 CCAGAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 125 AGATCC [GA]^GATC[CT]
CfoI 183 GCGC GCG^C
DraII 278 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 21 GACCTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 308 TGGCCA [CT]^GGCC[GA]
EarI 904 GAAGAG CTCTTC(1/4)
EcoO109I 278 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 613 GATATC GAT^ATC
FauI 143 GCGGG CCCGC(4/6)
FbaI 479 TGATCA T^GATCA
FspI 182 TGCGCA TGC^GCA
HapII 246 CCGG C^CGG
HhaI 183 GCGC GCG^C
HinP1I 183 GCGC G^CGC
HpaII 246 CCGG C^CGG
Ksp632I 904 GAAGAG CTCTTC(1/4)
MaeIII 361 GTCAC ^GT[ACGT]AC
MamI 64 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 125 AGATCC [GA]^GATC[CT]
MluNI 308 TGGCCA TGG^CCA
MscI 308 TGGCCA TGG^CCA
MseI 44 TTAA T^TAA
MslI 174 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 246 CCGG C^CGG
MvnI 142 CGCG CG^CG
NciI 246 CCGGG CC^[GC]GG
PflMI 54 CCAACTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 278 AGGACCT [GA]G^G[AT]CC[CT]
SmlI 43 CTTAAG C^T[CT][GA]AG
StuI 797 AGGCCT AGG^CCT
TaqI 578 TCGA T^CGA
Tru9I 44 TTAA T^TAA
Tsp45I 361 GTCAC ^GT[GC]AC
TthHB8I 578 TCGA T^CGA
Van91I 54 CCAACTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 125 AGATCC [GA]^GATC[CT]
XmnI 75 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 709 GTAC GT^AC
732 GTAC
AlwI 126 GATCC GGATC(4/5)
811 GATCC
AlwNI 323 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
669 CAGTGTCTG
AvaI 388 CTCGGG C^[CT]CG[GA]G
394 CCCGAG
BanII 105 GGGCTC G[GA]GC[CT]^C
391 GGGCCC
BbsI 10 GAAGAC GAAGAC(2/6)
399 GAAGAC
BpuAI 10 GAAGAC GAAGAC(2/6)
399 GAAGAC
BsaI 385 GGTCTC GGTCTC(1/5)
940 GGTCTC
BsmFI 296 GGGAC GGGAC(10/14)
135 GTCCC
BsoBI 388 CTCGGG C^[CT]CG[GA]G
394 CCCGAG
BsrDI 345 CATTGC GCAATG(2/0)
659 GCAATG
EcoT14I 272 CCAAGG C^C[AT][AT]GG
899 CCAAGG
HphI 269 TCACC GGTGA(8/7)
428 TCACC
PstI 679 CTGCAG CTGCA^G
875 CTGCAG
RsaI 709 GTAC GT^AC
732 GTAC
SfcI 679 CTGCAG C^T[GA][CT]AG
875 CTGCAG
StyI 272 CCAAGG C^C[AT][AT]GG
899 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI BanI BbvI BglI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MnlI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII Sau3AI Sau96I
ScrFI SfaNI TseI TspRI