Restriction map for
pF1KB8214
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CpoI DraI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MamI
MfeI MluI MroI MseI MslI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AfaI 639 GTAC GT^AC
AgeI 710 ACCGGT A^CCGGT
ApaI 241 GGGCCC GGGCC^C
Asp700I 393 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 247 GAGCTC G[AT]GC[AT]^C
BalI 47 TGGCCA TGG^CCA
BamHI 482 GGATCC G^GATCC
BcnI 357 CCGGG CC^[GC]GG
BglI 172 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 547 TACGTG [CT]AC^GT[GA]
BseRI 658 GAGGAG GAGGAG(10/8)
BsgI 451 CTGCAC GTGCAG(16/14)
BsiHKAI 247 GAGCTC G[AT]GC[AT]^C
BsmBI 598 GAGACG CGTCTC(1/5)
BsmFI 359 GGGAC GGGAC(10/14)
BspDI 751 ATCGAT AT^CGAT
BspMI 603 GCAGGT ACCTGC(4/8)
BsrDI 720 CATTGC GCAATG(2/0)
BsrI 76 ACTGG ACTGG(1/-1)
BssHII 525 GCGCGC G^CGCGC
BssSI 250 CTCGTG CACGAG(-5/-1)
BstAPI 507 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 292 CATCC GGATG(2/0)
BstYI 482 GGATCC [GA]^GATC[CT]
ClaI 751 ATCGAT AT^CGAT
DpnII 483 GATC ^GATC
DraIII 544 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 748 GACATCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 292 CATCC GGATG(9/13)
HgaI 600 GACGC GACGC(5/10)
MaeIII 757 GTCAC ^GT[ACGT]AC
MboI 483 GATC ^GATC
MflI 482 GGATCC [GA]^GATC[CT]
MluNI 47 TGGCCA TGG^CCA
MscI 47 TGGCCA TGG^CCA
NaeI 577 GCCGGC GCC^GGC
NciI 357 CCGGG CC^[GC]GG
NdeII 483 GATC ^GATC
NgoMIV 577 GCCGGC G^CCGGC
NlaIII 267 CATG CATG^
PinAI 710 ACCGGT A^CCGGT
PstI 322 CTGCAG CTGCA^G
RsaI 639 GTAC GT^AC
SacI 247 GAGCTC GAGCT^C
Sau3AI 483 GATC ^GATC
SfaNI 291 GCATC GCATC(5/9)
SfcI 322 CTGCAG C^T[GA][CT]AG
SfiI 171 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 63 CACCGGCG C[GA]^CCGG[CT]G
Tsp45I 757 GTCAC ^GT[GC]AC
TspRI 75 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 32 CCATAGAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 482 GGATCC [GA]^GATC[CT]
XmnI 393 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 483 GATCC GGATC(4/5)
482 GGATC
AlwNI 508 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
649 CAGAAGCTG
AvaI 244 CCCGAG C^[CT]CG[GA]G
259 CCCGAG
AvaII 360 GGACC G^G[AT]CC
384 GGTCC
BpmI 520 CTGGAG CTGGAG(16/14)
655 CTGGAG
BsaWI 626 ACCGGA [AT]^CCGG[AT]
710 ACCGGT
BsmAI 598 GAGAC GTCTC(1/5)
584 GTCTC
BsoBI 244 CCCGAG C^[CT]CG[GA]G
259 CCCGAG
DdeI 586 CTCAG C^T[ACGT]AG
671 CTGAG
EcoT14I 41 CCTTGG C^C[AT][AT]GG
50 CCAAGG
HinfI 37 GAGTC G^A[ACGT]TC
673 GAGTC
MaeII 428 ACGT A^CGT
548 ACGT
NotI 112 GCGGCCGC GC^GGCCGC
205 GCGGCCGC
PflMI 99 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCACTACGTGG
PleI 37 GAGTC GAGTC(4/5)
673 GAGTC
PpuMI 359 GGGACCC [GA]G^G[AT]CC[CT]
383 GGGTCCT
StyI 41 CCTTGG C^C[AT][AT]GG
50 CCAAGG
TaqI 533 TCGA T^CGA
752 TCGA
TthHB8I 533 TCGA T^CGA
752 TCGA
Van91I 99 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
543 CCACTACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BanII BbeI BbvI BmyI BsaHI
BsaJI BsiEI BsiYI BslI Bsp1286I
BsrBI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI KasI KspI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NlaIV PvuII
SacII Sau96I ScrFI SfoI TseI