##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TseI        Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AciI          345  CCGC                 CCGC(-3/-1)
AlwI          452  GGATC                GGATC(4/5)
BsaI          326  GAGACC               GGTCTC(1/5)
BspHI         144  TCATGA               T^CATGA
BspMI         181  GCAGGT               ACCTGC(4/8)
BstF5I        228  GGATG                GGATG(2/0)
DdeI          321  CTCAG                C^T[ACGT]AG
DraII         183  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          232  GAAGAG               CTCTTC(1/4)
EcoO109I      183  AGGTCCT              [GA]G^G[ACGT]CC[CT]
Fnu4HI        344  GCCGC                GC^[ACGT]GC
FokI          228  GGATG                GGATG(9/13)
ItaI          344  GCCGC                GC^[ACGT]GC
Ksp632I       232  GAAGAG               CTCTTC(1/4)
MaeIII        262  GTGAC                ^GT[ACGT]AC
NspI          240  GCATGT               [GA]CATG^[CT]
PpuMI         183  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          144  TCATGA               T^CATGA
StuI          123  AGGCCT               AGG^CCT
TaqI           62  TCGA                 T^CGA
Tsp45I        262  GTGAC                ^GT[GC]AC
TthHB8I        62  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          173  GTAC                 GT^AC
              404  GTAC                
BsgI           43  GTGCAG               GTGCAG(16/14)
              179  GTGCAG              
BsmAI         248  GAGAC                GTCTC(1/5)
              326  GAGAC               
BsrDI         331  CATTGC               GCAATG(2/0)
              257  GCAATG              
DpnII         312  GATC                 ^GATC
              453  GATC                
EaeI          284  TGGCCG               [CT]^GGCC[GA]
              342  TGGCCG              
HinfI         134  GATTC                G^A[ACGT]TC
              304  GATTC               
MboI          312  GATC                 ^GATC
              453  GATC                
MseI          107  TTAA                 T^TAA
              194  TTAA                
MslI          139  CATGTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              348  CACACTGGTG          
MwoI            4  GCGACGAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              335  GCATCATTGGC         
NdeII         312  GATC                 ^GATC
              453  GATC                
RsaI          173  GTAC                 GT^AC
              404  GTAC                
Sau3AI        312  GATC                 ^GATC
              453  GATC                
SfaNI          50  GCATC                GCATC(5/9)
              335  GCATC               
TfiI          134  GATTC                G^A[AT]TC
              304  GATTC               
Tru9I         107  TTAA                 T^TAA
              194  TTAA                
Tsp509I        58  AATT                 ^AATT
              447  AATT                
XcmI           85  CCAGTGAAGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              330  CCATTGCATCATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AvaII       BsiYI       BslI        BsrI        Cac8I       
Cfr13I      HaeIII      MboII       MnlI        NlaIII      
Sau96I      TspRI