Restriction map for
pF1KB8206
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 54 GGCGCC G[GA]^CG[CT]C
AfaI 757 GTAC GT^AC
AgeI 328 ACCGGT A^CCGGT
Alw44I 737 GTGCAC G^TGCAC
AlwNI 749 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 737 GTGCAC G^TGCAC
AvaII 316 GGTCC G^G[AT]CC
BanI 54 GGCGCC G^G[CT][GA]CC
BbeI 54 GGCGCC GGCGC^C
BbsI 783 GTCTTC GAAGAC(2/6)
BbvCI 354 CCTCAGC CCTCAGC(-5/-2)
BcgI 73 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 37 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 718 CTGGAG CTGGAG(16/14)
BpuAI 783 GTCTTC GAAGAC(2/6)
BsaHI 54 GGCGCC G[GA]^CG[CT]C
BsgI 147 CTGCAC GTGCAG(16/14)
BsmAI 136 GAGAC GTCTC(1/5)
BsmFI 797 GGGAC GGGAC(10/14)
BspMI 466 ACCTGC ACCTGC(4/8)
BsrFI 328 ACCGGT [GA]^CCGG[CT]
BstEII 579 GGTGACC G^GT[ACGT]ACC
BstPI 579 GGTGACC G^GT[ACGT]ACC
BstXI 306 CCATGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 328 ACCGGT [GA]^CCGG[CT]
EcoO65I 579 GGTGACC G^GT[ACGT]ACC
HaeII 54 GGCGCC [GA]GCGC^[CT]
Hin1I 54 GGCGCC G[GA]^CG[CT]C
KasI 54 GGCGCC G^GCGCC
MboII 784 TCTTC GAAGA(8/7)
MseI 622 TTAA T^TAA
MslI 535 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 54 GGCGCC GG^CGCC
NspI 301 GCATGC [GA]CATG^[CT]
PinAI 328 ACCGGT A^CCGGT
PvuII 486 CAGCTG CAG^CTG
RsaI 757 GTAC GT^AC
SfoI 54 GGCGCC GGC^GCC
SmaI 568 CCCGGG CCC^GGG
SphI 301 GCATGC GCATG^C
StuI 35 AGGCCT AGG^CCT
TaqI 72 TCGA T^CGA
Tru9I 622 TTAA T^TAA
TthHB8I 72 TCGA T^CGA
XmaCI 568 CCCGGG C^CCGGG
XmaI 568 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaI 5 CTCGGG C^[CT]CG[GA]G
568 CCCGGG
BcnI 568 CCCGG CC^[GC]GG
569 CCGGG
BglII 714 AGATCT A^GATCT
764 AGATCT
BseRI 477 CTCCTC GAGGAG(10/8)
31 GAGGAG
BsoBI 5 CTCGGG C^[CT]CG[GA]G
568 CCCGGG
BstYI 714 AGATCT [GA]^GATC[CT]
764 AGATCT
Cac8I 10 GCCAGC GC[ACGT]^[ACGT]GC
301 GCATGC
CfoI 55 GCGC GCG^C
218 GCGC
DdeI 244 CTCAG C^T[ACGT]AG
355 CTCAG
DpnII 715 GATC ^GATC
765 GATC
DsaI 306 CCATGG C^C[GA][CT]GG
773 CCATGG
EcoT14I 306 CCATGG C^C[AT][AT]GG
773 CCATGG
FauI 17 GCGGG CCCGC(4/6)
131 GCGGG
HhaI 55 GCGC GCG^C
218 GCGC
HinP1I 55 GCGC G^CGC
218 GCGC
HinfI 631 GACTC G^A[ACGT]TC
751 GAGTC
MaeIII 122 GTCAC ^GT[ACGT]AC
580 GTGAC
MboI 715 GATC ^GATC
765 GATC
MflI 714 AGATCT [GA]^GATC[CT]
764 AGATCT
NciI 568 CCCGG CC^[GC]GG
569 CCGGG
NcoI 306 CCATGG C^CATGG
773 CCATGG
NdeII 715 GATC ^GATC
765 GATC
NlaIV 54 GGCGCC GG[ACGT]^[ACGT]CC
429 GGCCCC
PleI 631 GACTC GAGTC(4/5)
751 GAGTC
SacI 163 GAGCTC GAGCT^C
643 GAGCTC
Sau3AI 715 GATC ^GATC
765 GATC
StyI 306 CCATGG C^C[AT][AT]GG
773 CCATGG
Tsp45I 122 GTCAC ^GT[GC]AC
580 GTGAC
XhoII 714 AGATCT [GA]^GATC[CT]
764 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BmyI BsaJI BsaWI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MnlI MspA1I MspI MvaI MwoI
NlaIII Sau96I ScrFI TseI TspRI