Restriction map for
pF1KB8189
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MscI
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 182 TCCGGA T^CCGGA
AocI 34 CCTCAGG CC^T[ACGT]AGG
BamHI 827 GGATCC G^GATCC
BbvI 118 GCTGC GCAGC(8/12)
BclI 294 TGATCA T^GATCA
BcnI 1172 CCGGG CC^[GC]GG
BsaWI 182 TCCGGA [AT]^CCGG[AT]
BseAI 182 TCCGGA T^CCGGA
BseRI 481 GAGGAG GAGGAG(10/8)
BsiEI 1310 CGACCG CG[GA][CT]^CG
BspEI 182 TCCGGA T^CCGGA
BspMI 207 ACCTGC ACCTGC(4/8)
BsrDI 941 GCAATG GCAATG(2/0)
BstEII 150 GGTAACC G^GT[ACGT]ACC
BstPI 150 GGTAACC G^GT[ACGT]ACC
BstYI 827 GGATCC [GA]^GATC[CT]
Bsu36I 34 CCTCAGG CC^T[ACGT]AGG
Cac8I 937 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 121 GCGC GCG^C
DraI 376 TTTAAA TTT^AAA
DsaI 581 CCGTGG C^C[GA][CT]GG
EarI 56 CTCTTC CTCTTC(1/4)
Eco81I 34 CCTCAGG CC^T[ACGT]AGG
EcoNI 959 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 150 GGTAACC G^GT[ACGT]ACC
FbaI 294 TGATCA T^GATCA
HhaI 121 GCGC GCG^C
HinP1I 121 GCGC G^CGC
Ksp632I 56 CTCTTC CTCTTC(1/4)
MflI 827 GGATCC [GA]^GATC[CT]
MroI 182 TCCGGA T^CCGGA
NciI 1172 CCGGG CC^[GC]GG
SapI 55 GCTCTTC GCTCTTC(1/4)
SmlI 370 CTCAAG C^T[CT][GA]AG
TaqI 630 TCGA T^CGA
TseI 118 GCTGC G^C[AT]GC
Tsp45I 472 GTGAC ^GT[GC]AC
TthHB8I 630 TCGA T^CGA
XhoII 827 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 415 CCGC CCGC(-3/-1)
115 GCGG
AfaI 174 GTAC GT^AC
1218 GTAC
AlwNI 37 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
142 CAGGAGCTG
AvaII 219 GGACC G^G[AT]CC
330 GGTCC
BbsI 310 GAAGAC GAAGAC(2/6)
870 GAAGAC
BpuAI 310 GAAGAC GAAGAC(2/6)
870 GAAGAC
BsaI 277 GAGACC GGTCTC(1/5)
367 GGTCTC
BsmFI 218 GGGAC GGGAC(10/14)
138 GTCCC
EcoRI 549 GAATTC G^AATTC
1247 GAATTC
HapII 183 CCGG C^CGG
1172 CCGG
HpaII 183 CCGG C^CGG
1172 CCGG
MslI 598 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
916 CACACTGGTG
MspI 183 CCGG C^CGG
1172 CCGG
NlaIII 325 CATG CATG^
1284 CATG
PflMI 141 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
457 CCAGATTTTGG
PleI 575 GACTC GAGTC(4/5)
1330 GAGTC
PpuMI 218 GGGACCT [GA]G^G[AT]CC[CT]
329 GGGTCCT
PvuII 795 CAGCTG CAG^CTG
879 CAGCTG
RsaI 174 GTAC GT^AC
1218 GTAC
Van91I 141 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
457 CCAGATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AspHI BanII BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MvaI NdeII NlaIV
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TfiI Tru9I Tsp509I
TspRI XmnI