################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AluI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstNI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvaI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 252 GTAC GT^AC AlwNI 262 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 45 GGGCCC GGGCC^C AspHI 243 GAGCAC G[AT]GC[AT]^C AspI 550 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 394 GAAGAC GAAGAC(2/6) BpuAI 394 GAAGAC GAAGAC(2/6) BsiHKAI 243 GAGCAC G[AT]GC[AT]^C BsmAI 79 GAGAC GTCTC(1/5) BspMI 210 GCAGGT ACCTGC(4/8) BstAPI 261 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 543 GGTCACC G^GT[ACGT]ACC BstF5I 218 CATCC GGATG(2/0) BstPI 543 GGTCACC G^GT[ACGT]ACC DpnII 330 GATC ^GATC EaeI 59 CGGCCA [CT]^GGCC[GA] EcoO65I 543 GGTCACC G^GT[ACGT]ACC EcoT14I 221 CCAAGG C^C[AT][AT]GG FauI 23 GCGGG CCCGC(4/6) FokI 218 CATCC GGATG(9/13) HphI 545 TCACC GGTGA(8/7) MaeII 398 ACGT A^CGT MaeIII 544 GTCAC ^GT[ACGT]AC MboI 330 GATC ^GATC NdeII 330 GATC ^GATC NspI 257 ACATGC [GA]CATG^[CT] PflFI 550 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 274 GAGTC GAGTC(4/5) PpuMI 212 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 252 GTAC GT^AC Sau3AI 330 GATC ^GATC SfaNI 457 GCATC GCATC(5/9) SmaI 48 CCCGGG CCC^GGG StyI 221 CCAAGG C^C[AT][AT]GG Tsp45I 544 GTCAC ^GT[GC]AC Tth111I 550 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 48 CCCGGG C^CCGGG XmaI 48 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 28 GGCGCC G[GA]^CG[CT]C 602 GGCGCC AvaI 48 CCCGGG C^[CT]CG[GA]G 97 CCCGAG BanI 28 GGCGCC G^G[CT][GA]CC 602 GGCGCC BanII 45 GGGCCC G[GA]GC[CT]^C 93 GAGCCC BbeI 28 GGCGCC GGCGC^C 602 GGCGCC BsaHI 28 GGCGCC G[GA]^CG[CT]C 602 GGCGCC BsoBI 48 CCCGGG C^[CT]CG[GA]G 97 CCCGAG BsrI 581 CCAGT ACTGG(1/-1) 587 CCAGT Cac8I 34 GCAGGC GC[ACGT]^[ACGT]GC 261 GCAGGC DraII 44 AGGGCCC [GA]G^G[ACGT]CC[CT] 212 AGGTCCC EcoO109I 44 AGGGCCC [GA]G^G[ACGT]CC[CT] 212 AGGTCCC Hin1I 28 GGCGCC G[GA]^CG[CT]C 602 GGCGCC KasI 28 GGCGCC G^GCGCC 602 GGCGCC NarI 28 GGCGCC GG^CGCC 602 GGCGCC SfoI 28 GGCGCC GGC^GCC 602 GGCGCC TfiI 282 GATTC G^A[AT]TC 297 GAATC TspRI 83 CAGTG CA[GC]TG[ACGT][ACGT]^ 588 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AvaII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI CfoI Cfr13I DdeI EarI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MboII MnlI MspI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI