Restriction map for pF1KB8183
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstNI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRII      EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MunI        MvaI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tsp509I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AfaI          252  GTAC                 GT^AC
AlwNI         262  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           45  GGGCCC               GGGCC^C
AspHI         243  GAGCAC               G[AT]GC[AT]^C
AspI          550  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          394  GAAGAC               GAAGAC(2/6)
BpuAI         394  GAAGAC               GAAGAC(2/6)
BsiHKAI       243  GAGCAC               G[AT]GC[AT]^C
BsmAI          79  GAGAC                GTCTC(1/5)
BspMI         210  GCAGGT               ACCTGC(4/8)
BstAPI        261  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        543  GGTCACC              G^GT[ACGT]ACC
BstF5I        218  CATCC                GGATG(2/0)
BstPI         543  GGTCACC              G^GT[ACGT]ACC
DpnII         330  GATC                 ^GATC
EaeI           59  CGGCCA               [CT]^GGCC[GA]
EcoO65I       543  GGTCACC              G^GT[ACGT]ACC
EcoT14I       221  CCAAGG               C^C[AT][AT]GG
FauI           23  GCGGG                CCCGC(4/6)
FokI          218  CATCC                GGATG(9/13)
HphI          545  TCACC                GGTGA(8/7)
MaeII         398  ACGT                 A^CGT
MaeIII        544  GTCAC                ^GT[ACGT]AC
MboI          330  GATC                 ^GATC
NdeII         330  GATC                 ^GATC
NspI          257  ACATGC               [GA]CATG^[CT]
PflFI         550  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          274  GAGTC                GAGTC(4/5)
PpuMI         212  AGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          252  GTAC                 GT^AC
Sau3AI        330  GATC                 ^GATC
SfaNI         457  GCATC                GCATC(5/9)
SmaI           48  CCCGGG               CCC^GGG
StyI          221  CCAAGG               C^C[AT][AT]GG
Tsp45I        544  GTCAC                ^GT[GC]AC
Tth111I       550  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          48  CCCGGG               C^CCGGG
XmaI           48  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI           28  GGCGCC               G[GA]^CG[CT]C
              602  GGCGCC              
AvaI           48  CCCGGG               C^[CT]CG[GA]G
               97  CCCGAG              
BanI           28  GGCGCC               G^G[CT][GA]CC
              602  GGCGCC              
BanII          45  GGGCCC               G[GA]GC[CT]^C
               93  GAGCCC              
BbeI           28  GGCGCC               GGCGC^C
              602  GGCGCC              
BsaHI          28  GGCGCC               G[GA]^CG[CT]C
              602  GGCGCC              
BsoBI          48  CCCGGG               C^[CT]CG[GA]G
               97  CCCGAG              
BsrI          581  CCAGT                ACTGG(1/-1)
              587  CCAGT               
Cac8I          34  GCAGGC               GC[ACGT]^[ACGT]GC
              261  GCAGGC              
DraII          44  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              212  AGGTCCC             
EcoO109I       44  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              212  AGGTCCC             
Hin1I          28  GGCGCC               G[GA]^CG[CT]C
              602  GGCGCC              
KasI           28  GGCGCC               G^GCGCC
              602  GGCGCC              
NarI           28  GGCGCC               GG^CGCC
              602  GGCGCC              
SfoI           28  GGCGCC               GGC^GCC
              602  GGCGCC              
TfiI          282  GATTC                G^A[AT]TC
              297  GAATC               
TspRI          83  CAGTG                CA[GC]TG[ACGT][ACGT]^
              588  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AvaII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       CfoI        Cfr13I      DdeI        
EarI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
Ksp632I     MboII       MnlI        MspI        MwoI        
NciI        NlaIII      NlaIV       Sau96I      ScrFI       
TseI        
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