Restriction map for
pF1KB8180
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1006 GTCTAC GT^[AC][GT]AC
AcsI 1090 AAATTC [GA]^AATT[CT]
AflII 958 CTTAAG C^TTAAG
Alw44I 872 GTGCAC G^TGCAC
AlwI 738 GATCC GGATC(4/5)
AlwNI 808 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1051 AGCGCT AGC^GCT
ApaLI 872 GTGCAC G^TGCAC
ApoI 1090 AAATTC [GA]^AATT[CT]
AvaI 833 CCCGGG C^[CT]CG[GA]G
BfrI 958 CTTAAG C^TTAAG
BglII 638 AGATCT A^GATCT
BseRI 1176 CTCCTC GAGGAG(10/8)
BsmI 550 GAATGC GAATGC(1/-1)
BsoBI 833 CCCGGG C^[CT]CG[GA]G
BspMI 932 ACCTGC ACCTGC(4/8)
BsrBI 1173 CCGCTC CCGCTC(-3/-3)
BsrFI 300 GCCGGC [GA]^CCGG[CT]
BstAPI 1239 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 305 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 300 GCCGGC [GA]^CCGG[CT]
EarI 592 CTCTTC CTCTTC(1/4)
Eco47III 1051 AGCGCT AGC^GCT
EcoT14I 695 CCATGG C^C[AT][AT]GG
HincII 256 GTCAAC GT[CT]^[GA]AC
HindII 256 GTCAAC GT[CT]^[GA]AC
HinfI 328 GAGTC G^A[ACGT]TC
Ksp632I 592 CTCTTC CTCTTC(1/4)
MaeII 275 ACGT A^CGT
NaeI 300 GCCGGC GCC^GGC
NcoI 695 CCATGG C^CATGG
NgoMIV 300 GCCGGC G^CCGGC
NheI 573 GCTAGC G^CTAGC
NspI 1239 GCATGT [GA]CATG^[CT]
PflMI 1029 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 328 GAGTC GAGTC(4/5)
PstI 539 CTGCAG CTGCA^G
PvuII 790 CAGCTG CAG^CTG
SapI 591 GCTCTTC GCTCTTC(1/4)
SmaI 833 CCCGGG CCC^GGG
SmlI 958 CTTAAG C^T[CT][GA]AG
StyI 695 CCATGG C^C[AT][AT]GG
Tsp509I 1091 AATT ^AATT
Van91I 1029 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 833 CCCGGG C^CCGGG
XmaI 833 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 120 GTAC GT^AC
1041 GTAC
ApaI 479 GGGCCC GGGCC^C
836 GGGCCC
BalI 518 TGGCCA TGG^CCA
692 TGGCCA
BanI 528 GGTGCC G^G[CT][GA]CC
745 GGCACC
BbsI 882 GAAGAC GAAGAC(2/6)
916 GTCTTC
BbvCI 8 CCTCAGC CCTCAGC(-5/-2)
752 CCTCAGC
BfaI 574 CTAG C^TAG
1166 CTAG
BpmI 71 CTCCAG CTGGAG(16/14)
802 CTGGAG
BpuAI 882 GAAGAC GAAGAC(2/6)
916 GTCTTC
BstYI 638 AGATCT [GA]^GATC[CT]
737 AGATCC
DpnII 639 GATC ^GATC
738 GATC
DsaI 483 CCGTGG C^C[GA][CT]GG
695 CCATGG
EcoNI 351 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
801 CCTGGAGCAGG
HaeII 776 AGCGCC [GA]GCGC^[CT]
1051 AGCGCT
HgaI 944 GCGTC GACGC(5/10)
1256 GCGTC
MaeI 574 CTAG C^TAG
1166 CTAG
MboI 639 GATC ^GATC
738 GATC
MflI 638 AGATCT [GA]^GATC[CT]
737 AGATCC
MluNI 518 TGGCCA TGG^CCA
692 TGGCCA
MscI 518 TGGCCA TGG^CCA
692 TGGCCA
MseI 959 TTAA T^TAA
1106 TTAA
MspA1I 429 CCGCTG C[AC]G^C[GT]G
790 CAGCTG
NdeII 639 GATC ^GATC
738 GATC
RsaI 120 GTAC GT^AC
1041 GTAC
Sau3AI 639 GATC ^GATC
738 GATC
SfcI 373 CTACAG C^T[GA][CT]AG
539 CTGCAG
Tru9I 959 TTAA T^TAA
1106 TTAA
XhoII 638 AGATCT [GA]^GATC[CT]
737 AGATCC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanII BbvI BcnI BglI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI XcmI