##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AcsI          332  GAATTC               [GA]^AATT[CT]
ApoI          332  GAATTC               [GA]^AATT[CT]
Asp700I       328  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         291  GGACC                G^G[AT]CC
BamHI         354  GGATCC               G^GATCC
BanII         317  GAGCCC               G[GA]GC[CT]^C
BmyI          317  GAGCCC               G[AGT]GC[ACT]^C
BseRI         200  GAGGAG               GAGGAG(10/8)
BsiYI         322  CCACAGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          322  CCACAGGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      317  GAGCCC               G[AGT]GC[ACT]^C
BspHI          11  TCATGA               T^CATGA
BsrDI         261  CATTGC               GCAATG(2/0)
BstF5I          3  GGATG                GGATG(2/0)
BstYI         354  GGATCC               [GA]^GATC[CT]
Cfr13I        291  GGACC                G^G[ACGT]CC
DpnII         355  GATC                 ^GATC
EcoRI         332  GAATTC               G^AATTC
FokI            3  GGATG                GGATG(9/13)
HaeIII         30  GGCC                 GG^CC
HphI          210  GGTGA                GGTGA(8/7)
MboI          355  GATC                 ^GATC
MflI          354  GGATCC               [GA]^GATC[CT]
NdeII         355  GATC                 ^GATC
RcaI           11  TCATGA               T^CATGA
Sau3AI        355  GATC                 ^GATC
Sau96I        291  GGACC                G^G[ACGT]CC
Tsp509I       333  AATT                 ^AATT
XhoII         354  GGATCC               [GA]^GATC[CT]
XmnI          328  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AlwI          355  GATCC                GGATC(4/5)
              354  GGATC               
AlwNI         161  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              269  CAGCCACTG           
BbsI          171  GAAGAC               GAAGAC(2/6)
              237  GAAGAC              
BpuAI         171  GAAGAC               GAAGAC(2/6)
              237  GAAGAC              
BsaJI          32  CCAAGG               C^C[ACGT][ACGT]GG
              131  CCAAGG              
BsrI          274  ACTGG                ACTGG(1/-1)
              294  CCAGT               
EcoT14I        32  CCAAGG               C^C[AT][AT]GG
              131  CCAAGG              
MwoI           79  GCAGAAGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              157  GCAACAGTGGC         
NlaIII         12  CATG                 CATG^
              148  CATG                
StyI           32  CCAAGG               C^C[AT][AT]GG
              131  CCAAGG              
XcmI           65  CCAAACAGGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              131  CCAAGGAGGGAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

BbvI        DdeI        Fnu4HI      ItaI        MaeIII      
MboII       MnlI        NlaIV       TseI        Tsp45I      
TspRI