Restriction map for
pF1KB8166
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BclI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KspI MaeII MamI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 590 GGTACC G^GTACC
AciI 843 GCGG CCGC(-3/-1)
AflII 1198 CTTAAG C^TTAAG
Asp718I 590 GGTACC G^GTACC
AspHI 623 GAGCAC G[AT]GC[AT]^C
AvaI 1418 CTCGAG C^[CT]CG[GA]G
BalI 1117 TGGCCA TGG^CCA
BamHI 387 GGATCC G^GATCC
BcgI 622 CGAGCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1068 CCGGG CC^[GC]GG
BfrI 1198 CTTAAG C^TTAAG
BglI 834 GCCAGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 461 CTGGAG CTGGAG(16/14)
BsaI 713 GAGACC GGTCTC(1/5)
BsiHKAI 623 GAGCAC G[AT]GC[AT]^C
BsoBI 1418 CTCGAG C^[CT]CG[GA]G
BsrFI 839 ACCGGC [GA]^CCGG[CT]
BstAPI 1843 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 476 GGTTACC G^GT[ACGT]ACC
BstPI 476 GGTTACC G^GT[ACGT]ACC
BstXI 674 CCAGATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 839 ACCGGC [GA]^CCGG[CT]
DraII 1845 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 423 CCATGG C^C[GA][CT]GG
EaeI 1117 TGGCCA [CT]^GGCC[GA]
EarI 1591 GAAGAG CTCTTC(1/4)
EcoNI 196 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1845 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 476 GGTTACC G^GT[ACGT]ACC
EcoRI 343 GAATTC G^AATTC
HaeII 668 AGCGCC [GA]GCGC^[CT]
KpnI 590 GGTACC GGTAC^C
Ksp632I 1591 GAAGAG CTCTTC(1/4)
MfeI 811 CAATTG C^AATTG
MluNI 1117 TGGCCA TGG^CCA
MscI 1117 TGGCCA TGG^CCA
MslI 1872 CATGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 811 CAATTG C^AATTG
NciI 1068 CCGGG CC^[GC]GG
NcoI 423 CCATGG C^CATGG
NdeI 220 CATATG CA^TATG
PaeR7I 1418 CTCGAG C^TCGAG
PflMI 891 CCATGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1845 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 777 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 44 AGTACT AGT^ACT
SexAI 1796 ACCTGGT A^CC[AT]GGT
Van91I 891 CCATGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1418 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1111 GTAGAC GT^[AC][GT]AC
1786 GTAGAC
BanI 590 GGTACC G^G[CT][GA]CC
1773 GGCACC
BanII 887 GAGCCC G[GA]GC[CT]^C
1140 GAGCCC
BciVI 850 GGATAC GTATCC(6/5)
1711 GTATCC
BglII 926 AGATCT A^GATCT
1248 AGATCT
BseRI 863 CTCCTC GAGGAG(10/8)
1324 GAGGAG
BsgI 446 CTGCAC GTGCAG(16/14)
1879 GTGCAG
CfoI 579 GCGC GCG^C
669 GCGC
HapII 840 CCGG C^CGG
1068 CCGG
HhaI 579 GCGC GCG^C
669 GCGC
HinP1I 579 GCGC G^CGC
669 GCGC
HindIII 16 AAGCTT A^AGCTT
1373 AAGCTT
HpaII 840 CCGG C^CGG
1068 CCGG
MspI 840 CCGG C^CGG
1068 CCGG
PstI 686 CTGCAG CTGCA^G
1279 CTGCAG
StuI 608 AGGCCT AGG^CCT
1495 AGGCCT
TaqI 621 TCGA T^CGA
1419 TCGA
TthHB8I 621 TCGA T^CGA
1419 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI