Restriction map for
pF1KB8160
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BsrBI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tru9I Tsp45I Tth111I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 750 GTAC GT^AC
AflIII 305 ACATGT A^C[GA][CT]GT
BbsI 301 GAAGAC GAAGAC(2/6)
BbvCI 708 CCTCAGC CCTCAGC(-5/-2)
BcnI 450 CCGGG CC^[GC]GG
BfaI 158 CTAG C^TAG
BglI 244 GCCAAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 141 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC
BpuAI 301 GAAGAC GAAGAC(2/6)
BseRI 341 CTCCTC GAGGAG(10/8)
BsmAI 696 GAGAC GTCTC(1/5)
Bsp1407I 749 TGTACA T^GTACA
BspHI 297 TCATGA T^CATGA
BspLU11I 305 ACATGT A^CATGT
BspMI 618 GCAGGT ACCTGC(4/8)
BsrFI 651 GCCGGT [GA]^CCGG[CT]
BsrGI 749 TGTACA T^GTACA
BssSI 664 CACGAG CACGAG(-5/-1)
BstAPI 561 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 527 TTCGAA TT^CGAA
BstF5I 521 GGATG GGATG(2/0)
BstYI 722 AGATCC [GA]^GATC[CT]
CelII 141 GCTCAGC GC^T[ACGT]AGC
Cfr10I 651 GCCGGT [GA]^CCGG[CT]
EaeI 56 TGGCCG [CT]^GGCC[GA]
EcoRI 772 GAATTC G^AATTC
EcoT22I 642 ATGCAT ATGCA^T
FokI 521 GGATG GGATG(9/13)
HaeII 145 AGCGCC [GA]GCGC^[CT]
HinfI 211 GACTC G^A[ACGT]TC
MaeI 158 CTAG C^TAG
MflI 722 AGATCC [GA]^GATC[CT]
NciI 450 CCGGG CC^[GC]GG
NsiI 642 ATGCAT ATGCA^T
NspV 527 TTCGAA TT^CGAA
PleI 211 GACTC GAGTC(4/5)
PvuII 37 CAGCTG CAG^CTG
RcaI 297 TCATGA T^CATGA
RsaI 750 GTAC GT^AC
SfuI 527 TTCGAA TT^CGAA
SspBI 749 TGTACA T^GTACA
StuI 356 AGGCCT AGG^CCT
XbaI 157 TCTAGA T^CTAGA
XcmI 275 CCATACCCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 722 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 457 GCGG CCGC(-3/-1)
558 GCGG
AlwNI 144 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
418 CAGGAGCTG
AocI 359 CCTCAGG CC^T[ACGT]AGG
516 CCTGAGG
AspHI 43 GAGCTC G[AT]GC[AT]^C
139 GAGCTC
BanI 268 GGCACC G^G[CT][GA]CC
648 GGTGCC
BanII 43 GAGCTC G[GA]GC[CT]^C
139 GAGCTC
BsgI 325 CTGCAC GTGCAG(16/14)
415 GTGCAG
BsiHKAI 43 GAGCTC G[AT]GC[AT]^C
139 GAGCTC
BsmI 289 GAATGC GAATGC(1/-1)
640 GAATGC
BsrDI 462 CATTGC GCAATG(2/0)
474 CATTGC
BsrI 118 ACTGG ACTGG(1/-1)
740 ACTGG
BstNI 207 CCTGG CC^[AT]GG
347 CCAGG
Bsu36I 359 CCTCAGG CC^T[ACGT]AGG
516 CCTGAGG
Cac8I 148 GCCTGC GC[ACGT]^[ACGT]GC
220 GCAAGC
CfoI 50 GCGC GCG^C
146 GCGC
Cfr13I 252 GGCCC G^G[ACGT]CC
513 GGCCC
DsaI 53 CCATGG C^C[GA][CT]GG
198 CCATGG
Eco81I 359 CCTCAGG CC^T[ACGT]AGG
516 CCTGAGG
EcoRII 207 CCTGG ^CC[AT]GG
347 CCAGG
HapII 450 CCGG C^CGG
652 CCGG
HhaI 50 GCGC GCG^C
146 GCGC
HinP1I 50 GCGC G^CGC
146 GCGC
HpaII 450 CCGG C^CGG
652 CCGG
MspI 450 CCGG C^CGG
652 CCGG
MvaI 207 CCTGG CC^[AT]GG
347 CCAGG
NcoI 53 CCATGG C^CATGG
198 CCATGG
NspI 305 ACATGT [GA]CATG^[CT]
746 GCATGT
PstI 34 CTGCAG CTGCA^G
547 CTGCAG
SacI 43 GAGCTC GAGCT^C
139 GAGCTC
Sau96I 252 GGCCC G^G[ACGT]CC
513 GGCCC
TaqI 84 TCGA T^CGA
528 TCGA
TthHB8I 84 TCGA T^CGA
528 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BssKI DdeI DpnII EcoT14I
Fnu4HI HaeIII HphI ItaI MboI
MboII MnlI MslI MspA1I MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI SfcI StyI TseI Tsp509I
TspRI