##################################
# NO CUT ENZYMES ( 196 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BmyI BpmI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssKI Bst1107I BstAPI
BstBI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CfoI Cfr10I Cfr13I CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT22I FauI FbaI FokI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaII HphI KasI KpnI KspI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 105 GTAC GT^AC
BbsI 541 GAAGAC GAAGAC(2/6)
BfaI 169 CTAG C^TAG
BlpI 436 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 436 GCTGAGC GC^T[ACGT]AGC
BpuAI 541 GAAGAC GAAGAC(2/6)
BsaAI 457 TACGTG [CT]AC^GT[GA]
BsaBI 189 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 249 CCGAAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 249 CCGAAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 236 GAGAC GTCTC(1/5)
BspDI 190 ATCGAT AT^CGAT
BsrI 369 ACTGG ACTGG(1/-1)
BssSI 479 CACGAG CACGAG(-5/-1)
CelII 436 GCTGAGC GC^T[ACGT]AGC
ClaI 190 ATCGAT AT^CGAT
DpnII 189 GATC ^GATC
EcoRI 27 GAATTC G^AATTC
EcoRV 193 GATATC GAT^ATC
EcoT14I 17 CCAAGG C^C[AT][AT]GG
HpaI 451 GTTAAC GTT^AAC
MaeI 169 CTAG C^TAG
MaeII 458 ACGT A^CGT
MaeIII 377 GTAAC ^GT[ACGT]AC
MamI 189 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 189 GATC ^GATC
MslI 51 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 189 GATC ^GATC
NlaIV 263 GGTTCC GG[ACGT]^[ACGT]CC
PleI 90 GACTC GAGTC(4/5)
RsaI 105 GTAC GT^AC
Sau3AI 189 GATC ^GATC
SfaNI 605 GATGC GCATC(5/9)
StyI 17 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 27 GAATTC [GA]^AATT[CT]
521 AAATTC
ApoI 27 GAATTC [GA]^AATT[CT]
521 AAATTC
DdeI 198 CTTAG C^T[ACGT]AG
437 CTGAG
EarI 418 GAAGAG CTCTTC(1/4)
570 GAAGAG
HincII 451 GTTAAC GT[CT]^[GA]AC
490 GTTGAC
HindII 451 GTTAAC GT[CT]^[GA]AC
490 GTTGAC
Ksp632I 418 GAAGAG CTCTTC(1/4)
570 GAAGAG
MspA1I 206 CAGCTG C[AC]G^C[GT]G
311 CAGCTG
MwoI 62 GCAGTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
304 GCAGCTACAGC
NlaIII 54 CATG CATG^
102 CATG
PvuII 206 CAGCTG CAG^CTG
311 CAGCTG
TaqI 191 TCGA T^CGA
525 TCGA
TfiI 295 GAATC G^A[AT]TC
593 GAATC
TspRI 63 CAGTG CA[GC]TG[ACGT][ACGT]^
442 CAGTG
TthHB8I 191 TCGA T^CGA
525 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BbvI Fnu4HI HinfI ItaI
MboII MnlI MseI SfcI Tru9I
TseI Tsp509I