Restriction map for
pF1KB8145
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MseI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 206 GTAGAC GT^[AC][GT]AC
AcsI 472 GAATTT [GA]^AATT[CT]
AflIII 332 ACATGT A^C[GA][CT]GT
AlwNI 230 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 244 CCTGAGG CC^T[ACGT]AGG
ApoI 472 GAATTT [GA]^AATT[CT]
Asp700I 319 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 58 CCCGAG C^[CT]CG[GA]G
BanII 55 GAGCCC G[GA]GC[CT]^C
BbvCI 103 GCTGAGG CCTCAGC(-5/-2)
BbvI 95 GCAGC GCAGC(8/12)
BsaBI 119 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 287 GAGACC GGTCTC(1/5)
BseRI 106 GAGGAG GAGGAG(10/8)
BsiYI 563 CCCATGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 563 CCCATGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 26 CGTCTC CGTCTC(1/5)
BsoBI 58 CCCGAG C^[CT]CG[GA]G
BspLU11I 332 ACATGT A^CATGT
BssSI 301 CTCGTG CACGAG(-5/-1)
BstEII 849 GGTCACC G^GT[ACGT]ACC
BstPI 849 GGTCACC G^GT[ACGT]ACC
Bsu36I 244 CCTGAGG CC^T[ACGT]AGG
Cfr13I 606 GGCCC G^G[ACGT]CC
DsaI 564 CCATGG C^C[GA][CT]GG
EarI 753 GAAGAG CTCTTC(1/4)
Eco81I 244 CCTGAGG CC^T[ACGT]AGG
EcoO65I 849 GGTCACC G^GT[ACGT]ACC
HaeII 859 AGCGCC [GA]GCGC^[CT]
HindIII 409 AAGCTT A^AGCTT
Ksp632I 753 GAAGAG CTCTTC(1/4)
MaeII 706 ACGT A^CGT
MamI 119 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 900 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 564 CCATGG C^CATGG
NspI 332 ACATGT [GA]CATG^[CT]
PshAI 876 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 606 GGCCC G^G[ACGT]CC
TaqI 83 TCGA T^CGA
TseI 95 GCAGC G^C[AT]GC
TthHB8I 83 TCGA T^CGA
XmnI 319 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 42 CGCG CG^CG
99 CGCG
BfaI 160 CTAG C^TAG
485 CTAG
BsrI 507 CCAGT ACTGG(1/-1)
784 CCAGT
BssKI 294 CCAGG ^CC[ACGT]GG
370 CCTGG
BstF5I 810 CATCC GGATG(2/0)
144 GGATG
BstNI 294 CCAGG CC^[AT]GG
370 CCTGG
BstUI 42 CGCG CG^CG
99 CGCG
BstYI 353 AGATCC [GA]^GATC[CT]
401 AGATCC
CfoI 98 GCGC GCG^C
860 GCGC
EcoRII 294 CCAGG ^CC[AT]GG
370 CCTGG
EcoT14I 564 CCATGG C^C[AT][AT]GG
602 CCAAGG
FauI 357 CCCGC CCCGC(4/6)
4 GCGGG
FokI 810 CATCC GGATG(9/13)
144 GGATG
HhaI 98 GCGC GCG^C
860 GCGC
HinP1I 98 GCGC G^CGC
860 GCGC
MaeI 160 CTAG C^TAG
485 CTAG
MflI 353 AGATCC [GA]^GATC[CT]
401 AGATCC
MvaI 294 CCAGG CC^[AT]GG
370 CCTGG
MvnI 42 CGCG CG^CG
99 CGCG
NlaIII 333 CATG CATG^
565 CATG
PleI 580 GACTC GAGTC(4/5)
61 GAGTC
PvuII 734 CAGCTG CAG^CTG
836 CAGCTG
ScrFI 294 CCAGG CC^[ACGT]GG
370 CCTGG
StyI 564 CCATGG C^C[AT][AT]GG
602 CCAAGG
TspRI 723 CACTG CA[GC]TG[ACGT][ACGT]^
785 CAGTG
XhoII 353 AGATCC [GA]^GATC[CT]
401 AGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AlwI BanI BmyI
BsaJI BsmAI Bsp1286I Cac8I DdeI
DpnII Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MwoI NdeII NlaIV Sau3AI
SfaNI TfiI Tsp45I Tsp509I