Restriction map for
pF1KB8142
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspHI AspI AvaI AvrII
BamHI BbeI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HindIII HpaI KasI KspI MamI
MfeI MluI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 94 GTCTAC GT^[AC][GT]AC
AccIII 353 TCCGGA T^CCGGA
AflIII 947 ACGTGT A^C[GA][CT]GT
Asp700I 255 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 149 TGGCCA TGG^CCA
BfaI 73 CTAG C^TAG
BsaWI 353 TCCGGA [AT]^CCGG[AT]
BseAI 353 TCCGGA T^CCGGA
BseRI 1051 GAGGAG GAGGAG(10/8)
BsgI 943 CTGCAC GTGCAG(16/14)
BsmBI 357 GAGACG CGTCTC(1/5)
BsmFI 500 GGGAC GGGAC(10/14)
BspEI 353 TCCGGA T^CCGGA
BspHI 989 TCATGA T^CATGA
BsrFI 194 GCCGGT [GA]^CCGG[CT]
BssSI 466 CACGAG CACGAG(-5/-1)
BstAPI 936 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 44 GGTTACC G^GT[ACGT]ACC
BstPI 44 GGTTACC G^GT[ACGT]ACC
BstYI 848 AGATCC [GA]^GATC[CT]
Cfr10I 194 GCCGGT [GA]^CCGG[CT]
DraIII 637 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 732 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 44 GGTTACC G^GT[ACGT]ACC
EcoT14I 284 CCAAGG C^C[AT][AT]GG
HaeII 135 GGCGCT [GA]GCGC^[CT]
HincII 754 GTCAAC GT[CT]^[GA]AC
HindII 754 GTCAAC GT[CT]^[GA]AC
HphI 627 GGTGA GGTGA(8/7)
MaeI 73 CTAG C^TAG
MflI 848 AGATCC [GA]^GATC[CT]
MluNI 149 TGGCCA TGG^CCA
MroI 353 TCCGGA T^CCGGA
MscI 149 TGGCCA TGG^CCA
PflMI 636 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 989 TCATGA T^CATGA
SphI 974 GCATGC GCATG^C
StuI 740 AGGCCT AGG^CCT
StyI 284 CCAAGG C^C[AT][AT]GG
TaqI 375 TCGA T^CGA
Tsp45I 58 GTCAC ^GT[GC]AC
TthHB8I 375 TCGA T^CGA
Van91I 636 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 848 AGATCC [GA]^GATC[CT]
XmnI 255 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 506 GGTACC G^GTACC
545 GGTACC
AcyI 359 GACGCC G[GA]^CG[CT]C
1151 GGCGTC
ApaI 888 GGGCCC GGGCC^C
1105 GGGCCC
Asp718I 506 GGTACC G^GTACC
545 GGTACC
AviII 686 TGCGCA TGC^GCA
971 TGCGCA
BbrPI 60 CACGTG CAC^GTG
946 CACGTG
BbsI 76 GAAGAC GAAGAC(2/6)
811 GTCTTC
BpuAI 76 GAAGAC GAAGAC(2/6)
811 GTCTTC
BsaAI 60 CACGTG [CT]AC^GT[GA]
946 CACGTG
BsaHI 359 GACGCC G[GA]^CG[CT]C
1151 GGCGTC
BsmAI 357 GAGAC GTCTC(1/5)
300 GTCTC
DdeI 190 CTGAG C^T[ACGT]AG
1133 CTCAG
EarI 421 CTCTTC CTCTTC(1/4)
790 CTCTTC
FspI 686 TGCGCA TGC^GCA
971 TGCGCA
Hin1I 359 GACGCC G[GA]^CG[CT]C
1151 GGCGTC
KpnI 506 GGTACC GGTAC^C
545 GGTACC
Ksp632I 421 CTCTTC CTCTTC(1/4)
790 CTCTTC
MaeII 61 ACGT A^CGT
947 ACGT
MslI 473 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
651 CATCCTGATG
NspI 761 ACATGC [GA]CATG^[CT]
974 GCATGC
PmaCI 60 CACGTG CAC^GTG
946 CACGTG
PmlI 60 CACGTG CAC^GTG
946 CACGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
TseI TspRI XcmI