Restriction map for
pF1KB8140
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# NO CUT ENZYMES ( 151 enzymes) #
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Acc65I AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstYI Bsu36I Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HindIII KasI KpnI
MamI MboI MfeI MflI MluI
MluNI MscI MslI MunI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
PvuI RcaI RsaI RsrII SacI
SalI SapI Sau3AI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 279 GACGTC GACGT^C
AccI 304 GTATAC GT^[AC][GT]AC
AccIII 170 TCCGGA T^CCGGA
AclI 231 AACGTT AA^CGTT
AcsI 697 GAATTC [GA]^AATT[CT]
AcyI 279 GACGTC G[GA]^CG[CT]C
ApoI 697 GAATTC [GA]^AATT[CT]
AvaII 99 GGACC G^G[AT]CC
BanII 765 GGGCTC G[GA]GC[CT]^C
BlpI 667 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 667 GCTCAGC GC^T[ACGT]AGC
BsaHI 279 GACGTC G[GA]^CG[CT]C
BsaWI 170 TCCGGA [AT]^CCGG[AT]
BseAI 170 TCCGGA T^CCGGA
BsmAI 269 GAGAC GTCTC(1/5)
BsmFI 758 GGGAC GGGAC(10/14)
BsmI 439 GCATTC GAATGC(1/-1)
BspEI 170 TCCGGA T^CCGGA
BsrBI 388 GAGCGG CCGCTC(-3/-3)
BsrDI 553 GCAATG GCAATG(2/0)
BsrI 642 ACTGG ACTGG(1/-1)
Bst1107I 304 GTATAC GTA^TAC
BstAPI 81 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 777 CATCC GGATG(2/0)
BstXI 635 CCAGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 304 GTATAC GTA^TAC
CelII 667 GCTCAGC GC^T[ACGT]AGC
CfoI 18 GCGC GCG^C
Cfr13I 99 GGACC G^G[ACGT]CC
DsaI 8 CCGCGG C^C[GA][CT]GG
EaeI 357 CGGCCA [CT]^GGCC[GA]
EarI 752 GAAGAG CTCTTC(1/4)
EcoRI 697 GAATTC G^AATTC
EcoT14I 743 CCAAGG C^C[AT][AT]GG
FokI 777 CATCC GGATG(9/13)
HgaI 147 GACGC GACGC(5/10)
HhaI 18 GCGC GCG^C
Hin1I 279 GACGTC G[GA]^CG[CT]C
HinP1I 18 GCGC G^CGC
HpaI 252 GTTAAC GTT^AAC
HphI 453 GGTGA GGTGA(8/7)
Ksp632I 752 GAAGAG CTCTTC(1/4)
KspI 8 CCGCGG CCGC^GG
MaeIII 616 GTTAC ^GT[ACGT]AC
MroI 170 TCCGGA T^CCGGA
NlaIII 361 CATG CATG^
PflMI 780 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 147 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 231 AACGTT AA^CGTT
PstI 88 CTGCAG CTGCA^G
SacII 8 CCGCGG CCGC^GG
Sau96I 99 GGACC G^G[ACGT]CC
SfcI 88 CTGCAG C^T[GA][CT]AG
StuI 258 AGGCCT AGG^CCT
StyI 743 CCAAGG C^C[AT][AT]GG
TfiI 220 GATTC G^A[AT]TC
Van91I 780 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 9 CGCG CG^CG
17 CGCG
AlwNI 82 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
583 CAGCAGCTG
BanI 40 GGTGCC G^G[CT][GA]CC
49 GGCACC
BbsI 154 GTCTTC GAAGAC(2/6)
399 GTCTTC
BfaI 67 CTAG C^TAG
151 CTAG
BmyI 48 GGGCAC G[AGT]GC[ACT]^C
765 GGGCTC
BpmI 589 CTGGAG CTGGAG(16/14)
730 CTGGAG
BpuAI 154 GTCTTC GAAGAC(2/6)
399 GTCTTC
BsgI 434 GTGCAG GTGCAG(16/14)
735 GTGCAG
Bsp1286I 48 GGGCAC G[AGT]GC[ACT]^C
765 GGGCTC
BstUI 9 CGCG CG^CG
17 CGCG
Cac8I 532 GCCTGC GC[ACGT]^[ACGT]GC
798 GCTGGC
HincII 252 GTTAAC GT[CT]^[GA]AC
313 GTCAAC
HindII 252 GTTAAC GT[CT]^[GA]AC
313 GTCAAC
MaeI 67 CTAG C^TAG
151 CTAG
MvnI 9 CGCG CG^CG
17 CGCG
PleI 13 GACTC GAGTC(4/5)
311 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstNI DdeI
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MaeII MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NlaIV PvuII ScrFI Tru9I
TseI Tsp509I TspRI