Restriction map for
pF1KB8129
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AviII BanI BbeI
BcgI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 767 GTCGAC GT^[AC][GT]AC
AcsI 541 GAATTT [GA]^AATT[CT]
AflIII 760 ACACGT A^C[GA][CT]GT
AhdI 759 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 315 GTGCAC G^TGCAC
ApaLI 315 GTGCAC G^TGCAC
ApoI 541 GAATTT [GA]^AATT[CT]
AspEI 759 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 315 GTGCAC G[AT]GC[AT]^C
AspI 280 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 15 CCTAGG C^CTAGG
BalI 836 TGGCCA TGG^CCA
BamHI 11 GGATCC G^GATCC
BanII 168 GGGCTC G[GA]GC[CT]^C
BbrPI 761 CACGTG CAC^GTG
BbsI 655 GAAGAC GAAGAC(2/6)
BbvCI 510 CCTCAGC CCTCAGC(-5/-2)
BlnI 15 CCTAGG C^CTAGG
BpuAI 655 GAAGAC GAAGAC(2/6)
BsaAI 761 CACGTG [CT]AC^GT[GA]
BsaBI 39 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 315 GTGCAC G[AT]GC[AT]^C
BsmBI 403 GAGACG CGTCTC(1/5)
BspHI 665 TCATGA T^CATGA
BspMI 108 GCAGGT ACCTGC(4/8)
BsrDI 261 CATTGC GCAATG(2/0)
BsrI 489 ACTGG ACTGG(1/-1)
BstEII 639 GGTCACC G^GT[ACGT]ACC
BstPI 639 GGTCACC G^GT[ACGT]ACC
BstXI 644 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 411 GCGC GCG^C
DraIII 488 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 759 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 898 CTCTTC CTCTTC(1/4)
EcoO65I 639 GGTCACC G^GT[ACGT]ACC
FauI 825 GCGGG CCCGC(4/6)
HhaI 411 GCGC GCG^C
HinP1I 411 GCGC G^CGC
HincII 767 GTCGAC GT[CT]^[GA]AC
HindII 767 GTCGAC GT[CT]^[GA]AC
Ksp632I 898 CTCTTC CTCTTC(1/4)
MaeII 762 ACGT A^CGT
MaeIII 640 GTCAC ^GT[ACGT]AC
MamI 39 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 836 TGGCCA TGG^CCA
MscI 836 TGGCCA TGG^CCA
NcoI 650 CCATGG C^CATGG
NspI 483 GCATGC [GA]CATG^[CT]
PflFI 280 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 761 CACGTG CAC^GTG
PmlI 761 CACGTG CAC^GTG
RcaI 665 TCATGA T^CATGA
SalI 767 GTCGAC G^TCGAC
SapI 897 GCTCTTC GCTCTTC(1/4)
SmaI 746 CCCGGG CCC^GGG
SphI 483 GCATGC GCATG^C
Tsp45I 640 GTCAC ^GT[GC]AC
Tsp509I 542 AATT ^AATT
Tth111I 280 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 746 CCCGGG C^CCGGG
XmaI 746 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 254 CTCGGG C^[CT]CG[GA]G
746 CCCGGG
BseRI 727 GAGGAG GAGGAG(10/8)
1015 GAGGAG
BsoBI 254 CTCGGG C^[CT]CG[GA]G
746 CCCGGG
BstAPI 888 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
951 GCAGACCCTGC
BstYI 11 GGATCC [GA]^GATC[CT]
38 AGATCC
Cac8I 467 GCCTGC GC[ACGT]^[ACGT]GC
483 GCATGC
DsaI 48 CCACGG C^C[GA][CT]GG
650 CCATGG
EaeI 201 TGGCCG [CT]^GGCC[GA]
836 TGGCCA
EcoNI 15 CCTAGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
102 CCTCCAGCAGG
EcoT14I 15 CCTAGG C^C[AT][AT]GG
650 CCATGG
HapII 441 CCGG C^CGG
747 CCGG
HinfI 182 GACTC G^A[ACGT]TC
735 GAGTC
HpaII 441 CCGG C^CGG
747 CCGG
MflI 11 GGATCC [GA]^GATC[CT]
38 AGATCC
MslI 660 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
666 CATGACTGTG
MspI 441 CCGG C^CGG
747 CCGG
PleI 182 GACTC GAGTC(4/5)
735 GAGTC
PpuMI 98 AGGACCT [GA]G^G[AT]CC[CT]
123 AGGACCC
SmlI 772 CTTGAG C^T[CT][GA]AG
847 CTCAAG
StyI 15 CCTAGG C^C[AT][AT]GG
650 CCATGG
TaqI 348 TCGA T^CGA
768 TCGA
TspRI 446 CACTG CA[GC]TG[ACGT][ACGT]^
488 CACTG
TthHB8I 348 TCGA T^CGA
768 TCGA
XhoII 11 GGATCC [GA]^GATC[CT]
38 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HphI ItaI
MaeI MboI MboII MnlI MspA1I
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TseI